Highly Efficient Production of Soluble Proteins from Insoluble Inclusion Bodies by a Two-Step-Denaturing and Refolding Method

• Zhong Yang ,

  Contributed equally to this work with: Zhong Yang, Linlin Zhang

  Affiliations State Key Laboratory of Genetic Engineering, Department of Microbiology, School of Life Sciences, Fudan University, Shanghai, China, State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai, China

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• Linlin Zhang ,

  Contributed equally to this work with: Zhong Yang, Linlin Zhang

  Affiliations State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, CAS, Shanghai, China, State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai, China

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• Yan Zhang,

  Affiliation State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, CAS, Shanghai, China

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• Ting Zhang,

  Affiliation State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai, China

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• Yanye Feng,

  Affiliations State Key Laboratory of Genetic Engineering, Department of Microbiology, School of Life Sciences, Fudan University, Shanghai, China, State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai, China

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• Xiuxiu Lu,

  Affiliation State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, CAS, Shanghai, China

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• Wenxian Lan,

  Affiliation State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, CAS, Shanghai, China

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• Jufang Wang,

  Affiliation School of Bioscience and Bioengineering, South China University of Science and Technology, Guangzhou, China

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• Houming Wu,

  Affiliation State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, CAS, Shanghai, China

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• Chunyang Cao ,

  * E-mail: ccao@mail.sioc.ac.cn (CC); xnwang88@gmail.com (XW)

  Affiliation State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, CAS, Shanghai, China

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• Xiaoning Wang

  * E-mail: ccao@mail.sioc.ac.cn (CC); xnwang88@gmail.com (XW)

  Affiliations State Key Laboratory of Genetic Engineering, Department of Microbiology, School of Life Sciences, Fudan University, Shanghai, China, School of Bioscience and Bioengineering, South China University of Science and Technology, Guangzhou, China

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