TIM, a targeted insertional mutagenesis method utilizing CRISPR/Cas9 in Chlamydomonas reinhardtii

• Tyler Picariello ,

  Contributed equally to this work with: Tyler Picariello, Yuqing Hou

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Validation, Writing – original draft, Writing – review & editing

  Current address: Sanofi-Genzyme, Framingham, Massachusetts, United States of America

  Affiliation Division of Cell Biology and Imaging, Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America

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• Yuqing Hou ,

  Contributed equally to this work with: Tyler Picariello, Yuqing Hou

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Validation, Writing – original draft, Writing – review & editing

  Affiliation Division of Cell Biology and Imaging, Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America

  http://orcid.org/0000-0002-7038-675X

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• Tomohiro Kubo,

  Roles Funding acquisition, Methodology, Validation, Writing – review & editing

  Affiliation Department of Anatomy and Structural Biology, Interdisciplinary Graduate School, University of Yamanashi, Chuo, Yamanashi, Japan

  ⨯
• Nathan A. McNeill,

  Roles Formal analysis, Validation, Writing – review & editing

  Affiliation Division of Cell Biology and Imaging, Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America

  http://orcid.org/0000-0001-9688-5728

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• Haru-aki Yanagisawa,

  Roles Methodology, Validation, Writing – review & editing

  Affiliation Graduate School of Medicine, The University of Tokyo, Tokyo, Japan

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• Toshiyuki Oda,

  Roles Formal analysis, Funding acquisition, Validation, Writing – review & editing

  Affiliation Department of Anatomy and Structural Biology, Interdisciplinary Graduate School, University of Yamanashi, Chuo, Yamanashi, Japan

  ⨯
• George B. Witman

  Roles Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Writing – original draft, Writing – review & editing

  * E-mail: george.witman@umassmed.edu

  Affiliation Division of Cell Biology and Imaging, Department of Radiology, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America

  http://orcid.org/0000-0002-9497-9218

  ⨯