Genetic Transformation of a Clinical (Genital Tract), Plasmid-Free Isolate of Chlamydia trachomatis: Engineering the Plasmid as a Cloning Vector

Open Access

Peer-reviewed

Research Article

Yibing Wang,

Affiliation Molecular Microbiology Group, University of Southampton, Southampton, United Kingdom
⨯
Simona Kahane,

Affiliation Department of Virology, Ben Gurion University of the Negev, Beer Sheva, Israel
⨯
Lesley T. Cutcliffe,

Affiliation Molecular Microbiology Group, University of Southampton, Southampton, United Kingdom
⨯
Rachel J. Skilton,

Affiliation Molecular Microbiology Group, University of Southampton, Southampton, United Kingdom
⨯
Paul R. Lambden,

Affiliation Molecular Microbiology Group, University of Southampton, Southampton, United Kingdom
⨯
Kenneth Persson,

Affiliation Department of Laboratory Medicine, Malmo University Hospital, Malmo, Sweden
⨯
Carina Bjartling,

Affiliation Department of Obstetrics and Gynaecology, Malmo University Hospital, Malmo, Sweden
⨯
Ian N. Clarke

* E-mail: inc@soton.ac.uk

Affiliation Molecular Microbiology Group, University of Southampton, Southampton, United Kingdom
⨯

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Chlamydia trachomatis
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Plasmid construction
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Chlamydia infection
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Chlamydia
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Penicillin
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Centrifugation
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Glycogens
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Iodine
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