Phenotypes of Non-Attached Pseudomonas aeruginosa Aggregates Resemble Surface Attached Biofilm

Morten Alhede; Kasper Nørskov Kragh; Klaus Qvortrup; Marie Allesen-Holm; Maria van Gennip; Louise D. Christensen; Peter Østrup Jensen; Anne K. Nielsen; Matt Parsek; Dan Wozniak; Søren Molin; Tim Tolker-Nielsen; Niels Høiby; Michael Givskov; Thomas Bjarnsholt

doi:10.1371/journal.pone.0027943

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Peer-reviewed

Research Article

Phenotypes of Non-Attached Pseudomonas aeruginosa Aggregates Resemble Surface Attached Biofilm

Morten Alhede,

Affiliation Institute for International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark
⨯
Kasper Nørskov Kragh,

Affiliation Institute for International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark
⨯
Klaus Qvortrup,

Affiliation Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark
⨯
Marie Allesen-Holm,

Affiliation Institute for International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark
⨯
Maria van Gennip,

Affiliation Institute for International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark
⨯
Louise D. Christensen,

Affiliation Institute for International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark
⨯
Peter Østrup Jensen,

Affiliation Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark
⨯
Anne K. Nielsen,

Affiliation Institute for International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark
⨯
Matt Parsek,

Affiliation Department of Microbiology, University of Washington, Seattle, Washington, United States of America
⨯
Dan Wozniak,

Affiliation Division Infectious Disease, Center for Microbial Interface Biology, The Ohio State University, Columbus, Ohio, United States of America
⨯
Søren Molin,

Affiliation Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark
⨯
Tim Tolker-Nielsen,

Affiliation Institute for International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark
⨯
Niels Høiby,

Affiliation Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark
⨯
Michael Givskov,

Affiliation Department of Microbiology, University of Washington, Seattle, Washington, United States of America
⨯
Thomas Bjarnsholt

* E-mail: tbjarnsholt@sund.ku.dk

Affiliations Department of Microbiology, University of Washington, Seattle, Washington, United States of America, Division Infectious Disease, Center for Microbial Interface Biology, The Ohio State University, Columbus, Ohio, United States of America
⨯

Phenotypes of Non-Attached Pseudomonas aeruginosa Aggregates Resemble Surface Attached Biofilm

Morten Alhede,
Kasper Nørskov Kragh,
Klaus Qvortrup,
Marie Allesen-Holm,
Maria van Gennip,
Louise D. Christensen,
Peter Østrup Jensen,
Anne K. Nielsen,
Matt Parsek,
Dan Wozniak

Published: November 21, 2011
https://doi.org/10.1371/journal.pone.0027943

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Missing paragraph

Posted by tbjarnsholt on 27 Feb 2012 at 08:45 GMT

.
http://plosone.org/article/info:doi/10.1371/journal.pone.0027943#article1.body1.sec1.p4

Interestingly, a recent paper by Schleheck et al. showed that P. aeruginosa PAO1 preferentially grows as aggregates in liquid batch cultures. During the growth phase it was found that 90% of the total biomass consisted of particles ranging from 5 to 600•m embedded in a fine structured extracellular matrix consisting of DNA [Schleheck D, Barraud N, Klebensberger J, Webb JS, McDougald D, et al. (2009) Pseudomonas aeruginosa PAO1 preferentially grows as aggregates in liquid batch cultures and disperses upon starvation. PLoS One 4: e5513.]."

No competing interests declared.

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Phenotypes of Non-Attached Pseudomonas aeruginosa Aggregates Resemble Surface Attached Biofilm

Phenotypes of Non-Attached Pseudomonas aeruginosa Aggregates Resemble Surface Attached Biofilm

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Missing paragraph

Posted by tbjarnsholt on 27 Feb 2012 at 08:45 GMT