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Referee Comments: Referee 1 (Andrew Cal)

Posted by PLOS_ONE_Group on 21 Mar 2008 at 16:58 GMT

Referee 1's Review (Andrew Cal):

A great resource. The specificity of the spl11 knockdown was especially beautiful. Was there any correlation between copy number and efficacy of knock-down? Also, were all regenerates confirmed to have amiRNA sequence, or just the hpt gene? Partial insertion could skew your % of transgenic lines with efficient silencing downward.
Nice work.

N.B. These are the comments made by the referee when reviewing an earlier version of this paper. Prior to publication the manuscript has been revised in light of these comments and to address other editorial requirements