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Recommended concentration of Sortase

Posted by Cameron_Neylon on 06 Nov 2008 at 17:13 GMT

40 nM
http://plosone.org/article/info:doi/10.1371/journal.pone.0001164#article1.body1.sec2.p2

Others have reported using much higher Sortase concentrations to do ligation reactions. More recently we have seen significant variations in the concentration of Sortase required to get good ligation yields between different protein targets. It is not clear at the moment whether this is a variation in the activity of different Sortase preps or activity against different protein target constructs. Where there are problems we have always been able to get good yields by increasing the Sortase concentration to around 1 uM.