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closeImpact; previous imprinting assays in mouse brain
Posted by rschulz on 14 Dec 2008 at 20:49 GMT
The imprinting status of seven known imprinted genes have not been previously reported in neonatal brain, including the paternally expressed Peg13, Sgce and Nap1l5 (Table 1; Figures S1.6–S1.8) [26], [27] and the maternally expressed Gtl2 (Meg3), Impact, H19 and Cdkn1c (P57KIP2) (Table 1; Figures S1.9–S1.11) [28]–[31].
http://plosone.org/article/info:doi/10.1371/journal.pone.0003839#article1.body1.sec2.sec2.p3
Impact is a paternally expressed imprinted gene (http://www.ncbi.nlm.nih.gov/pubmed/9256468). H19 and Cdkn1c have had their imprinting status confirmed in mouse newborn brain (http://www.ncbi.nlm.nih.gov/pubmed/9732553 and http://www.ncbi.nlm.nih.gov/pubmed/16702402 , respectively). The imprinting status of the remaining five genes (Peg13, Sgce, Nap1l5, Gtl2, Impact) has previously been confirmed in mouse brain (http://www.ncbi.nlm.nih.gov/pubmed/17704508 , http://www.ncbi.nlm.nih.gov/pubmed/10757814 , http://www.ncbi.nlm.nih.gov/pubmed/12670997 , http://www.ncbi.nlm.nih.gov/pubmed/10759892 and http://www.ncbi.nlm.nih.gov/pubmed/9256468 , respectively).
RE: Impact; previous imprinting assays in mouse brain
andrewclark replied to rschulz on 15 Dec 2008 at 00:29 GMT
It is indeed true that Impact is a paternally expressed imprinted gene (http://www.ncbi.nlm.nih.g...), and we did cite this paper as Reference # 29. We also show this fact correctly in supporting Figure 1.9 and Table 1. Unfortunately there was a typographical error in the text, which we will correct in an addendum. Thanks for catching this.
Regarding H19, we already cited the commenter’s noted paper as Reference # 30. Please note that this paper reported the imprinting status of H19 in fetal brain, not neonatal brain.
For Cdkn1c, in this paper, we find that “Cdkn1c is faithfully imprinted in all mouse tissues in which it is expressed, from early embryonic development through adulthood.” But the paper is silent on specific experimental evidence regarding whether Cdkn1c is imprinted in neonatal brain. The title of this paper is “Elongation of the Kcnq1ot1 transcript is required for genomic imprinting of neighboring genes”.
We already mentioned that the last 5 genes mentioned in the comment are known imprinted genes in mouse. Since most imprinted genes show some degree of tissue- or developmental stage-specific expression, especially in placenta and different stages of brains (fetal brain, neonatal brain and adult brain). We are saying that the imprinting status in neonatal brain had not been unambiguously reported in the literature.
RE: RE: Impact; previous imprinting assays in mouse brain
rschulz replied to andrewclark on 16 Dec 2008 at 10:42 GMT
Regarding H19, Hemberger et al. (http://www.ncbi.nlm.nih.g...) state:
'To assess the imprinting status of H19 and Igf2 in the CNS, we dissected the region of the hindbrain containing the VMR [...] For H19, mouse fetuses were dissected on e14 (n=2), e17 (n=2) and e19 (n=4). In
addition, two MSM BC1 mice were dissected on day 2 after birth. At all stages examined, H19 was expressed from the maternal allele only in both VMR and PC.'
Regarding Cdkn1c, Mancini-Dinardo et al. (http://www.ncbi.nlm.nih.g...) state:
'We have examined the imprinting status of Cdkn1c in multiple tissues in newborn and adult mice bearing each of three mutations. We determined that when the DMR[Delta], Prom[Delta], and Term mutations were inherited paternally, every tissue analyzed (brain, heart, liver, lung, spleen, kidney) displayed biallelic expression of Cdkn1c (data not shown).'