PCR-free whole exome sequencing: Cost-effective and efficient in detecting rare mutations

Izumi Yamaguchi; Takashi Watanabe; Osamu Ohara; Yoshinori Hasegawa

doi:10.1371/journal.pone.0222562

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Open Access

Peer-reviewed

Research Article

PCR-free whole exome sequencing: Cost-effective and efficient in detecting rare mutations

Izumi Yamaguchi,

Roles Investigation, Methodology

Affiliation Laboratory of Clinical Omics Research, Department of Applied Genomics, Kazusa DNA Research Institute, Chiba, Japan
⨯
Takashi Watanabe,

Roles Investigation, Methodology

Affiliation Laboratory of Clinical Omics Research, Department of Applied Genomics, Kazusa DNA Research Institute, Chiba, Japan
⨯
Osamu Ohara,

Roles Conceptualization, Funding acquisition, Supervision

Affiliation Laboratory of Clinical Omics Research, Department of Applied Genomics, Kazusa DNA Research Institute, Chiba, Japan
⨯
Yoshinori Hasegawa

Roles Conceptualization, Data curation, Formal analysis, Methodology, Project administration, Resources, Software, Supervision, Validation, Writing – original draft, Writing – review & editing

* E-mail: yhasega@kazusa.or.jp

Affiliation Laboratory of Clinical Omics Research, Department of Applied Genomics, Kazusa DNA Research Institute, Chiba, Japan

http://orcid.org/0000-0003-2904-6611

⨯

PCR-free whole exome sequencing: Cost-effective and efficient in detecting rare mutations

Izumi Yamaguchi,
Takashi Watanabe,
Osamu Ohara,
Yoshinori Hasegawa

Published: September 13, 2019
https://doi.org/10.1371/journal.pone.0222562

About the Authors

Izumi Yamaguchi

Roles Investigation, Methodology

Affiliation Laboratory of Clinical Omics Research, Department of Applied Genomics, Kazusa DNA Research Institute, Chiba, Japan

Takashi Watanabe

Roles Investigation, Methodology

Affiliation Laboratory of Clinical Omics Research, Department of Applied Genomics, Kazusa DNA Research Institute, Chiba, Japan

Osamu Ohara

Roles Conceptualization, Funding acquisition, Supervision

Affiliation Laboratory of Clinical Omics Research, Department of Applied Genomics, Kazusa DNA Research Institute, Chiba, Japan

Yoshinori Hasegawa

Roles Conceptualization, Data curation, Formal analysis, Methodology, Project administration, Resources, Software, Supervision, Validation, Writing – original draft, Writing – review & editing

* E-mail: yhasega@kazusa.or.jp

Affiliation Laboratory of Clinical Omics Research, Department of Applied Genomics, Kazusa DNA Research Institute, Chiba, Japan

http://orcid.org/0000-0003-2904-6611

Competing Interests

The authors have declared that no competing interests exist.

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DNA libraries
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Polymerase chain reaction
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DNA fragment ligation
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Mutation detection
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Ultrasonication
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DNA hybridization
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DNA electrophoresis
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Next-generation sequencing
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