Three-Dimensional Imaging of the Intracellular Fate of Plasmid DNA and Transgene Expression: ZsGreen1 and Tissue Clearing Method CUBIC Are an Optimal Combination for Multicolor Deep Imaging in Murine Tissues

Shintaro Fumoto; Koyo Nishimura; Koyo Nishida; Shigeru Kawakami

doi:10.1371/journal.pone.0148233

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Open Access

Peer-reviewed

Research Article

Three-Dimensional Imaging of the Intracellular Fate of Plasmid DNA and Transgene Expression: ZsGreen1 and Tissue Clearing Method CUBIC Are an Optimal Combination for Multicolor Deep Imaging in Murine Tissues

Shintaro Fumoto ,

Contributed equally to this work with: Shintaro Fumoto, Koyo Nishimura

* E-mail: sfumoto@nagasaki-u.ac.jp (SF); skawakam@nagasaki-u.ac.jp (SK)

Affiliation Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan
⨯
Koyo Nishimura ,

Contributed equally to this work with: Shintaro Fumoto, Koyo Nishimura

Affiliation Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan
⨯
Koyo Nishida,

Affiliation Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan
⨯
Shigeru Kawakami

* E-mail: sfumoto@nagasaki-u.ac.jp (SF); skawakam@nagasaki-u.ac.jp (SK)

Affiliation Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan
⨯

Three-Dimensional Imaging of the Intracellular Fate of Plasmid DNA and Transgene Expression: ZsGreen1 and Tissue Clearing Method CUBIC Are an Optimal Combination for Multicolor Deep Imaging in Murine Tissues

Shintaro Fumoto,
Koyo Nishimura,
Koyo Nishida,
Shigeru Kawakami

Published: January 29, 2016
https://doi.org/10.1371/journal.pone.0148233

About the Authors

Shintaro Fumoto

Contributed equally to this work with: Shintaro Fumoto, Koyo Nishimura

* E-mail: sfumoto@nagasaki-u.ac.jp (SF); skawakam@nagasaki-u.ac.jp (SK)

Affiliation Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan

Koyo Nishimura

Contributed equally to this work with: Shintaro Fumoto, Koyo Nishimura

Affiliation Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan

Koyo Nishida

Affiliation Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan

Shigeru Kawakami

* E-mail: sfumoto@nagasaki-u.ac.jp (SF); skawakam@nagasaki-u.ac.jp (SK)

Affiliation Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: SF SK. Performed the experiments: SF K. Nishimura. Analyzed the data: SF K. Nishimura K. Nishida. Contributed reagents/materials/analysis tools: SF K. Nishimura. Wrote the paper: SF K. Nishida SK.

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Tissue clearing
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Lasers
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Confocal laser microscopy
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Green fluorescent protein
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Plasmid construction
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Optical lenses
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Hydrodynamics
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Tissue distribution
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