The Orthology Clause in the Next Generation Sequencing Era: Novel Reference Genes Identified by RNA-seq in Humans Improve Normalization of Neonatal Equine Ovary RT-qPCR Data

Dragos Scarlet; Reinhard Ertl; Christine Aurich; Ralf Steinborn

doi:10.1371/journal.pone.0142122

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Open Access

Peer-reviewed

Research Article

The Orthology Clause in the Next Generation Sequencing Era: Novel Reference Genes Identified by RNA-seq in Humans Improve Normalization of Neonatal Equine Ovary RT-qPCR Data

Dragos Scarlet ,

* E-mail: dragos.scarlet@vetmeduni.ac.at

Affiliation Centre for Artificial Insemination and Embryo Transfer, University of Veterinary Medicine, Vienna, Austria
⨯
Reinhard Ertl,

Affiliation Genomics Core Facility, VetCore, University of Veterinary Medicine, Vienna, Austria
⨯
Christine Aurich,

Affiliation Centre for Artificial Insemination and Embryo Transfer, University of Veterinary Medicine, Vienna, Austria
⨯
Ralf Steinborn

Affiliation Genomics Core Facility, VetCore, University of Veterinary Medicine, Vienna, Austria
⨯

The Orthology Clause in the Next Generation Sequencing Era: Novel Reference Genes Identified by RNA-seq in Humans Improve Normalization of Neonatal Equine Ovary RT-qPCR Data

Dragos Scarlet,
Reinhard Ertl,
Christine Aurich,
Ralf Steinborn

Published: November 4, 2015
https://doi.org/10.1371/journal.pone.0142122

About the Authors

Dragos Scarlet

* E-mail: dragos.scarlet@vetmeduni.ac.at

Affiliation Centre for Artificial Insemination and Embryo Transfer, University of Veterinary Medicine, Vienna, Austria

Reinhard Ertl

Affiliation Genomics Core Facility, VetCore, University of Veterinary Medicine, Vienna, Austria

Christine Aurich

Affiliation Centre for Artificial Insemination and Embryo Transfer, University of Veterinary Medicine, Vienna, Austria

Ralf Steinborn

Affiliation Genomics Core Facility, VetCore, University of Veterinary Medicine, Vienna, Austria

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: DS RS. Performed the experiments: RE. Analyzed the data: DS RE CA RS. Wrote the paper: DS RE CA RS. Study initiation: DS CA RS. Supervised the study: CA RS.

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Ovaries
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Equines
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Gene expression
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Computer software
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Horses
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Introns
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Exon mapping
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Deoxyribonucleases
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