CRISPR/Cas9-Mediated Genomic Deletion of the Beta-1, 4 N-acetylgalactosaminyltransferase 1 Gene in Murine P19 Embryonal Carcinoma Cells Results in Low Sensitivity to Botulinum Neurotoxin Type C

Kentaro Tsukamoto; Chikako Ozeki; Tomoko Kohda; Takao Tsuji

doi:10.1371/journal.pone.0132363

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Peer-reviewed

Research Article

CRISPR/Cas9-Mediated Genomic Deletion of the Beta-1, 4 N-acetylgalactosaminyltransferase 1 Gene in Murine P19 Embryonal Carcinoma Cells Results in Low Sensitivity to Botulinum Neurotoxin Type C

Kentaro Tsukamoto ,

* E-mail: tsuka-k@fujita-hu.ac.jp

Affiliation Department of Microbiology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan
⨯
Chikako Ozeki,

Affiliation Department of Microbiology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan
⨯
Tomoko Kohda,

Affiliation Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan
⨯
Takao Tsuji

Affiliation Department of Microbiology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan
⨯

CRISPR/Cas9-Mediated Genomic Deletion of the Beta-1, 4 N-acetylgalactosaminyltransferase 1 Gene in Murine P19 Embryonal Carcinoma Cells Results in Low Sensitivity to Botulinum Neurotoxin Type C

Kentaro Tsukamoto,
Chikako Ozeki,
Tomoko Kohda,
Takao Tsuji

Published: July 15, 2015
https://doi.org/10.1371/journal.pone.0132363

About the Authors

Kentaro Tsukamoto

* E-mail: tsuka-k@fujita-hu.ac.jp

Affiliation Department of Microbiology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan

Chikako Ozeki

Affiliation Department of Microbiology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan

Tomoko Kohda

Affiliation Department of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan

Takao Tsuji

Affiliation Department of Microbiology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: KT TT. Performed the experiments: KT CO. Analyzed the data: KT CO. Contributed reagents/materials/analysis tools: KT CO TK. Wrote the paper: KT.

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Sphingolipids
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Neurons
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Small interfering RNA
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P19 cells
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Botulinum toxin
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Transfection
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Vector cloning
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CRISPR
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