About the Authors

Alexander R. Mendenhall

rbrent@fhcrc.org (RB); amendenh@fhcrc.org (ARM)

Affiliation Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America

Patricia M. Tedesco

Affiliation Institute for Behavioral Genetics, University of Colorado, Boulder, Colorado, United States of America

Bryan Sands

Affiliation Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America

Thomas E. Johnson

Affiliations Institute for Behavioral Genetics, University of Colorado, Boulder, Colorado, United States of America, Department of Integrative Physiology, University of Colorado, Boulder, Colorado, United States of America, Biofrontiers Institute, University of Colorado, Boulder, Colorado, United States of America

Roger Brent

rbrent@fhcrc.org (RB); amendenh@fhcrc.org (ARM)

Affiliation Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: ARM TEJ RB. Performed the experiments: ARM BS PMT. Analyzed the data: ARM PMT BS RB. Contributed reagents/materials/analysis tools: ARM BS PMT. Wrote the paper: ARM RB. Provided lab for initial work: TEJ. Designed the microscopy experiments: ARM. Designed other initial experiments: ARM TEJ. Designed later experiments: ARM TEJ RB. Measured copy number in reporter strains, generated and outcrossed the new multicopy reporter strains, and sequenced the constructions and transgenes in the single copy strains: PMT. Generated the DNA encoding the daf-21 reporter genes: BS. Generated new single copy strains, performed crosses to generate RBW2 and the F1 heterozygotes, performed flow and microscopy experiments, and carried out statistical analyses: RB and ARM. Interpreted these results, wrote the paper, and guarantee the integrity of its results and conclusions: ARM.