TFIIB Is Only ∼9 Å Away from the 5'-End of a Trimeric RNA Primer in a Functional RNA Polymerase II Preinitiation Complex

Matthew J. Bick; Sohail Malik; Arkady Mustaev; Seth A. Darst

doi:10.1371/journal.pone.0119007

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Peer-reviewed

Research Article

TFIIB Is Only ∼9 Å Away from the 5'-End of a Trimeric RNA Primer in a Functional RNA Polymerase II Preinitiation Complex

Matthew J. Bick,

Affiliation Laboratory of Molecular Biophysics, The Rockefeller University, New York, NY, United States of America
⨯
Sohail Malik,

Affiliation Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY, United States of America
⨯
Arkady Mustaev,

Affiliation Department of Chemistry and Environmental Sciences, New Jersey Institute of Technology, University Heights, Newark, NJ, United States of America
⨯
Seth A. Darst

* E-mail: darst@mail.rockefeller.edu

Affiliation Laboratory of Molecular Biophysics, The Rockefeller University, New York, NY, United States of America
⨯

TFIIB Is Only ∼9 Å Away from the 5'-End of a Trimeric RNA Primer in a Functional RNA Polymerase II Preinitiation Complex

Matthew J. Bick,
Sohail Malik,
Arkady Mustaev,
Seth A. Darst

Published: March 16, 2015
https://doi.org/10.1371/journal.pone.0119007

About the Authors

Matthew J. Bick

Affiliation Laboratory of Molecular Biophysics, The Rockefeller University, New York, NY, United States of America

Sohail Malik

Affiliation Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY, United States of America

Arkady Mustaev

Affiliation Department of Chemistry and Environmental Sciences, New Jersey Institute of Technology, University Heights, Newark, NJ, United States of America

Seth A. Darst

* E-mail: darst@mail.rockefeller.edu

Affiliation Laboratory of Molecular Biophysics, The Rockefeller University, New York, NY, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: MJB SM SAD. Performed the experiments: MJB SM. Analyzed the data: MJB SM SAD. Contributed reagents/materials/analysis tools: MJB SM AM SAD. Wrote the paper: MJB SM SAD.

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Cross-linking
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Nucleotides
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DNA transcription
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RNA structure
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Transcription factors
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Eukaryota
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RNA polymerase
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Radiolabeling
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