About the Authors

Cathal J. McElgunn

c.mcelgunn@lumora.co.uk

Affiliation Lumora Ltd, Ely, Cambridgeshire, United Kingdom

Clint R. Pereira

Affiliation Lumora Ltd, Ely, Cambridgeshire, United Kingdom

Nicholas J. Parham

Affiliation Public Health England, Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Cambridge, Cambridgeshire, United Kingdom

James E. Smythe

Affiliation Lumora Ltd, Ely, Cambridgeshire, United Kingdom

Michael J. Wigglesworth

Affiliation Lumora Ltd, Ely, Cambridgeshire, United Kingdom

Anna Smielewska

Affiliation Public Health England, Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Cambridge, Cambridgeshire, United Kingdom

Surendra A. Parmar

Affiliation Public Health England, Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Cambridge, Cambridgeshire, United Kingdom

Olga A. Gandelman

Affiliation Lumora Ltd, Ely, Cambridgeshire, United Kingdom

Nicholas M. Brown

Affiliation Public Health England, Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Cambridge, Cambridgeshire, United Kingdom

Laurence C. Tisi

Affiliation Lumora Ltd, Ely, Cambridgeshire, United Kingdom

Martin D. Curran

Affiliation Public Health England, Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Cambridge, Cambridgeshire, United Kingdom

Competing Interests

LCT and OAG are inventors of Bioluminescent Assay in Real-Time (BART). LCT co-founded Lumora Ltd, a company that has full rights for the use of the BART patent. Patent name: Method for Determining the Amount of Template Nucleic Acid Present in a Sample and number WO2006010948. A further pending patent is ongoing. CJM, CRP and LCT are the inventors of heat elution sample preparation technology. CJM, CRP, JES, MJW, OAG and LCT declare financial interests in Lumora Ltd. The following authors were all employees at Lumora Ltd: CJM, CRP, JES, MJW, OAG and LCT, whose company funded this study. There are no further patents, products in development or marketed products to declare. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: CJM CRP NJP MDC SAP OAG NMB LCT. Performed the experiments: CJM CRP NJP MDC LCT AS JES MJW. Analyzed the data: CJM CRP NJP MDC JES LCT. Contributed reagents/materials/analysis tools: CJM CRP JES MJW SAP OAG. Wrote the paper: CJM NJP MDC CRP LCT.