Evaluation of Reference Genes for RT qPCR Analyses of Structure-Specific and Hormone Regulated Gene Expression in Physcomitrella patens Gametophytes

Aude Le Bail; Sebastian Scholz; Benedikt Kost

doi:10.1371/journal.pone.0070998

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Open Access

Peer-reviewed

Research Article

Evaluation of Reference Genes for RT qPCR Analyses of Structure-Specific and Hormone Regulated Gene Expression in Physcomitrella patens Gametophytes

Aude Le Bail,

Affiliation Cell Biology & Erlangen Center of Plant Science (ECROPS), Friedrich Alexander University, Erlangen, Germany
⨯
Sebastian Scholz,

Current address: Plant Developmental Biology, Technical University of Munich, Freising, Germany

Affiliation Cell Biology & Erlangen Center of Plant Science (ECROPS), Friedrich Alexander University, Erlangen, Germany
⨯
Benedikt Kost

* E-mail: benedikt.kost@fau.de

Affiliation Cell Biology & Erlangen Center of Plant Science (ECROPS), Friedrich Alexander University, Erlangen, Germany
⨯

Evaluation of Reference Genes for RT qPCR Analyses of Structure-Specific and Hormone Regulated Gene Expression in Physcomitrella patens Gametophytes

Aude Le Bail,
Sebastian Scholz,
Benedikt Kost

Published: August 9, 2013
https://doi.org/10.1371/journal.pone.0070998

About the Authors

Aude Le Bail

Affiliation Cell Biology & Erlangen Center of Plant Science (ECROPS), Friedrich Alexander University, Erlangen, Germany

Sebastian Scholz

Current address: Plant Developmental Biology, Technical University of Munich, Freising, Germany

Affiliation Cell Biology & Erlangen Center of Plant Science (ECROPS), Friedrich Alexander University, Erlangen, Germany

Benedikt Kost

* E-mail: benedikt.kost@fau.de

Affiliation Cell Biology & Erlangen Center of Plant Science (ECROPS), Friedrich Alexander University, Erlangen, Germany

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: ALB. Performed the experiments: SS. Analyzed the data: ALB SS BK. Contributed reagents/materials/analysis tools: ALB BK. Wrote the paper: ALB BK.

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Gene expression
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Hormone transport
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RNA structure
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Hormonal therapy
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Auxins
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Gene regulation
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RNA analysis
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Computer software
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