A Filtering Method to Generate High Quality Short Reads Using Illumina Paired-End Technology

A. Murat Eren; Joseph H. Vineis; Hilary G. Morrison; Mitchell L. Sogin

doi:10.1371/journal.pone.0066643

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Open Access

Peer-reviewed

Research Article

A Filtering Method to Generate High Quality Short Reads Using Illumina Paired-End Technology

A. Murat Eren ,

Contributed equally to this work with: A. Murat Eren, Joseph H. Vineis

Affiliation Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts, United States of America
⨯
Joseph H. Vineis ,

Contributed equally to this work with: A. Murat Eren, Joseph H. Vineis

Affiliation Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts, United States of America
⨯
Hilary G. Morrison,

Affiliation Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts, United States of America
⨯
Mitchell L. Sogin

* E-mail: sogin@mbl.edu.

Affiliation Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts, United States of America
⨯

A Filtering Method to Generate High Quality Short Reads Using Illumina Paired-End Technology

A. Murat Eren,
Joseph H. Vineis,
Hilary G. Morrison,
Mitchell L. Sogin

Published: June 17, 2013
https://doi.org/10.1371/journal.pone.0066643

About the Authors

A. Murat Eren

Contributed equally to this work with: A. Murat Eren, Joseph H. Vineis

Affiliation Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts, United States of America

Joseph H. Vineis

Contributed equally to this work with: A. Murat Eren, Joseph H. Vineis

Affiliation Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts, United States of America

Hilary G. Morrison

Affiliation Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts, United States of America

Mitchell L. Sogin

* E-mail: sogin@mbl.edu.

Affiliation Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, Massachusetts, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: AME JHV HGM MLS. Analyzed the data: AME JHV. Wrote the paper: AME JHV. Implemented the bioinformatics tools: AME. Designed the primers: HGM. Performed the library preperation and sequencing: JHV. Edited the manuscript: HGM MLS.

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Polymerase chain reaction
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Nucleotide sequencing
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Ribosomal RNA
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DNA isolation
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Marker genes
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Metagenomics
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Shotgun sequencing
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Gene sequencing
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