Identification of Subunit-Subunit Interaction Sites in αA-WT Crystallin and Mutant αA-G98R Crystallin Using Isotope-Labeled Cross-Linker and Mass Spectrometry

Rama Kannan; Puttur Santhoshkumar; Brian P. Mooney; K. Krishna Sharma

doi:10.1371/journal.pone.0065610

Loading metrics

Open Access

Peer-reviewed

Research Article

Identification of Subunit-Subunit Interaction Sites in αA-WT Crystallin and Mutant αA-G98R Crystallin Using Isotope-Labeled Cross-Linker and Mass Spectrometry

Rama Kannan,

Affiliation Department of Biochemistry, University of Missouri, Columbia, Missouri, United States of America
⨯
Puttur Santhoshkumar,

Affiliation Department of Ophthalmology, University of Missouri, Columbia, Missouri, United States of America
⨯
Brian P. Mooney,

Affiliations Department of Biochemistry, University of Missouri, Columbia, Missouri, United States of America, University of Missouri, Charles W. Gehrke Proteomics Center, Columbia, Missouri, United States of America
⨯
K. Krishna Sharma

* E-mail: Sharmak@health.missouri.edu

Affiliations Department of Biochemistry, University of Missouri, Columbia, Missouri, United States of America, Department of Ophthalmology, University of Missouri, Columbia, Missouri, United States of America
⨯

Identification of Subunit-Subunit Interaction Sites in αA-WT Crystallin and Mutant αA-G98R Crystallin Using Isotope-Labeled Cross-Linker and Mass Spectrometry

Rama Kannan,
Puttur Santhoshkumar,
Brian P. Mooney,
K. Krishna Sharma

Published: June 5, 2013
https://doi.org/10.1371/journal.pone.0065610

About the Authors

Rama Kannan

Affiliation Department of Biochemistry, University of Missouri, Columbia, Missouri, United States of America

Puttur Santhoshkumar

Affiliation Department of Ophthalmology, University of Missouri, Columbia, Missouri, United States of America

Brian P. Mooney

Affiliations Department of Biochemistry, University of Missouri, Columbia, Missouri, United States of America, University of Missouri, Charles W. Gehrke Proteomics Center, Columbia, Missouri, United States of America

K. Krishna Sharma

* E-mail: Sharmak@health.missouri.edu

Affiliations Department of Biochemistry, University of Missouri, Columbia, Missouri, United States of America, Department of Ophthalmology, University of Missouri, Columbia, Missouri, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: KKS RK PS BM. Performed the experiments: RK BM. Analyzed the data: RK BM PS KKS. Contributed reagents/materials/analysis tools: KKS. Wrote the paper: RK PS BM KKS.

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Protein interactions
Is the Subject Area "Protein interactions" applicable to this article?

Thanks for your feedback.
Cross-linking
Is the Subject Area "Cross-linking" applicable to this article?

Thanks for your feedback.
Cataracts
Is the Subject Area "Cataracts" applicable to this article?

Thanks for your feedback.
Lysine
Is the Subject Area "Lysine" applicable to this article?

Thanks for your feedback.
Serine proteases
Is the Subject Area "Serine proteases" applicable to this article?

Thanks for your feedback.
Light
Is the Subject Area "Light" applicable to this article?

Thanks for your feedback.
Liquid chromatography-mass spectrometry
Is the Subject Area "Liquid chromatography-mass spectrometry" applicable to this article?

Thanks for your feedback.
Computer software
Is the Subject Area "Computer software" applicable to this article?

Thanks for your feedback.