High Concentrations of L-Ascorbic Acid Specifically Inhibit the Growth of Human Leukemic Cells via Downregulation of HIF-1α Transcription

Hiroshi Kawada; Mitsuyo Kaneko; Masakazu Sawanobori; Tomoko Uno; Hideyuki Matsuzawa; Yoshihiko Nakamura; Hiromichi Matsushita; Kiyoshi Ando

doi:10.1371/journal.pone.0062717

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Peer-reviewed

Research Article

High Concentrations of L-Ascorbic Acid Specifically Inhibit the Growth of Human Leukemic Cells via Downregulation of HIF-1α Transcription

Hiroshi Kawada ,

Contributed equally to this work with: Hiroshi Kawada, Mitsuyo Kaneko

* E-mail: hkawada@is.icc.u-tokai.ac.jp

Affiliations Division of Hematology/Oncology, Department of Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan, Research Center for Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan
⨯
Mitsuyo Kaneko ,

Contributed equally to this work with: Hiroshi Kawada, Mitsuyo Kaneko

Affiliations Division of Hematology/Oncology, Department of Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan, Research Center for Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan
⨯
Masakazu Sawanobori,

Affiliation Division of Hematology/Oncology, Department of Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan
⨯
Tomoko Uno,

Affiliation Research Center for Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan
⨯
Hideyuki Matsuzawa,

Affiliation Research Center for Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan
⨯
Yoshihiko Nakamura,

Affiliation Research Center for Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan
⨯
Hiromichi Matsushita,

Affiliations Research Center for Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan, Department of Laboratory Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan
⨯
Kiyoshi Ando

Affiliations Division of Hematology/Oncology, Department of Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan, Research Center for Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan
⨯

High Concentrations of L-Ascorbic Acid Specifically Inhibit the Growth of Human Leukemic Cells via Downregulation of HIF-1α Transcription

Hiroshi Kawada,
Mitsuyo Kaneko,
Masakazu Sawanobori,
Tomoko Uno,
Hideyuki Matsuzawa,
Yoshihiko Nakamura,
Hiromichi Matsushita,
Kiyoshi Ando

Published: April 23, 2013
https://doi.org/10.1371/journal.pone.0062717

About the Authors

Hiroshi Kawada

Contributed equally to this work with: Hiroshi Kawada, Mitsuyo Kaneko

* E-mail: hkawada@is.icc.u-tokai.ac.jp

Affiliations Division of Hematology/Oncology, Department of Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan, Research Center for Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan

Mitsuyo Kaneko

Contributed equally to this work with: Hiroshi Kawada, Mitsuyo Kaneko

Masakazu Sawanobori

Affiliation Division of Hematology/Oncology, Department of Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan

Tomoko Uno

Affiliation Research Center for Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan

Hideyuki Matsuzawa

Affiliation Research Center for Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan

Yoshihiko Nakamura

Affiliation Research Center for Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan

Hiromichi Matsushita

Affiliations Research Center for Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan, Department of Laboratory Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan

Kiyoshi Ando

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: HK MK. Performed the experiments: MK TU H. Matsuzawa YN. Analyzed the data: HK MK KA. Contributed reagents/materials/analysis tools: MS H. Matsushita KA. Wrote the paper: HK.

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Leukemia
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Cancers and neoplasms
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Cancer treatment
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HL60 cells
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Transcription factors
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Apoptosis
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Catalases
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Malignant tumors
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