About the Authors

Nalan Liv

Affiliation Department of Imaging Science and Technology, Faculty of Applied Sciences, Delft University of Technology, Delft, The Netherlands

A. Christiaan Zonnevylle

Affiliation Department of Imaging Science and Technology, Faculty of Applied Sciences, Delft University of Technology, Delft, The Netherlands

Angela C. Narvaez

Affiliation Department of Imaging Science and Technology, Faculty of Applied Sciences, Delft University of Technology, Delft, The Netherlands

Andries P. J. Effting

Affiliation DELMIC BV, Delft, The Netherlands

Philip W. Voorneveld

Affiliation Department of Gastroenterology and Hepatology, Leiden University Medical Center, Leiden, The Netherlands

Miriam S. Lucas

Affiliation Electron Microscopy ETH Zurich - EMEZ, ETH Zurich, Zurich, Switzerland

James C. Hardwick

Affiliation Department of Gastroenterology and Hepatology, Leiden University Medical Center, Leiden, The Netherlands

Roger A. Wepf

Affiliation Electron Microscopy ETH Zurich - EMEZ, ETH Zurich, Zurich, Switzerland

Pieter Kruit

Affiliation Department of Imaging Science and Technology, Faculty of Applied Sciences, Delft University of Technology, Delft, The Netherlands

Jacob P. Hoogenboom

j.p.hoogenboom@tudelft.nl

Affiliation Department of Imaging Science and Technology, Faculty of Applied Sciences, Delft University of Technology, Delft, The Netherlands

Competing Interests

DELMIC BV may profit from publication of the results of this study. A.P.J.E. is shareholder and employee of DELMIC BV. A.C.Z. is shareholder in DELMIC. P.K. and J.P.H. are shareholders and members of the advisory board to DELMIC. Vacuum-compatible immersion oil was supplied by DELMIC BV for this study. DELMIC BV is developing a commercial product based on the prototype microscope used in this study. This study was partly supported by NanoNextNL. There are no further patents, products in development or marketed products to declare. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials, as detailed online in the guide for authors.

Author Contributions

Designed and constructed the integrated microscope: ACZ JPH. Constructed the optical microscope: NL ACZ ACN APJE JPH. Conceived and designed the experiments: NL ACZ PK JPH. Performed the experiments: NL ACZ ACN. Analyzed the data: NL PWV JPH. Contributed reagents/materials/analysis tools: PWV MSL JCH RAW. Wrote the paper: NL JPH.