About the Authors

Masaki Kinehara

Affiliation Laboratory of Stem Cell Cultures, Department of Disease Bioresources Research, National Institute of Biomedical Innovation, Ibaraki, Osaka, Japan

Suguru Kawamura

Affiliation Laboratory of Stem Cell Cultures, Department of Disease Bioresources Research, National Institute of Biomedical Innovation, Ibaraki, Osaka, Japan

Daiki Tateyama

Affiliation Laboratory of Stem Cell Cultures, Department of Disease Bioresources Research, National Institute of Biomedical Innovation, Ibaraki, Osaka, Japan

Mika Suga

Affiliation Laboratory of Stem Cell Cultures, Department of Disease Bioresources Research, National Institute of Biomedical Innovation, Ibaraki, Osaka, Japan

Hiroko Matsumura

Affiliation Laboratory of Stem Cell Cultures, Department of Disease Bioresources Research, National Institute of Biomedical Innovation, Ibaraki, Osaka, Japan

Sumiyo Mimura

Affiliation Laboratory of Stem Cell Cultures, Department of Disease Bioresources Research, National Institute of Biomedical Innovation, Ibaraki, Osaka, Japan

Noriko Hirayama

Affiliation Laboratory of Cell Cultures, Department of Disease Bioresources Research, National Institute of Biomedical Innovation, Ibaraki, Osaka, Japan

Mitsuhi Hirata

Affiliation Laboratory of Stem Cell Cultures, Department of Disease Bioresources Research, National Institute of Biomedical Innovation, Ibaraki, Osaka, Japan

Kozue Uchio-Yamada

Affiliation Laboratory of Animal Models for Human Diseases, Department of Disease Bioresources Research, National Institute of Biomedical Innovation, Ibaraki, Osaka, Japan

Arihiro Kohara

Affiliation Laboratory of Cell Cultures, Department of Disease Bioresources Research, National Institute of Biomedical Innovation, Ibaraki, Osaka, Japan

Kana Yanagihara

Affiliation Laboratory of Stem Cell Cultures, Department of Disease Bioresources Research, National Institute of Biomedical Innovation, Ibaraki, Osaka, Japan

Miho K. Furue

mkfurue@nibio.go.jp

Affiliation Laboratory of Stem Cell Cultures, Department of Disease Bioresources Research, National Institute of Biomedical Innovation, Ibaraki, Osaka, Japan

Competing Interests

The authors have read the journal's policy and have the following conflicts: One of the authors, (MKF) has declared a financial interest in a company, Cell Science & Technology Institute Corporation (Sendai, Japan) whose product, a basal medium ESF was used in this study. However, the licensing fee is less than $10,000 per year. This does not alter the authors adherence to all the PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: MK MKF. Performed the experiments: MK SK DT MS HM SM NH MH KUY AK KY. Analyzed the data: MK. Wrote the paper: MK MKF.