About the Authors

Louise Hyslop

Affiliation Newcastle Fertility Centre, International Centre for Life, Newcastle upon Tyne, England, United Kingdom

Nilendran Prathalingam

Affiliation Institute for Ageing and Health, Newcastle University, Newcastle Fertility Centre, Centre for Life, Newcastle upon Tyne, England, United Kingdom

Lynne Nowak

Affiliation Newcastle Fertility Centre, International Centre for Life, Newcastle upon Tyne, England, United Kingdom

Jeanette Fenwick

Affiliation Newcastle Fertility Centre, International Centre for Life, Newcastle upon Tyne, England, United Kingdom

Steve Harbottle

Current address: Cambridge IVF, Kefford House, Maris Lane, Trumpington, Cambridge, England, United Kingdom

Affiliation Newcastle Fertility Centre, International Centre for Life, Newcastle upon Tyne, England, United Kingdom

Samantha Byerley

Affiliation Newcastle Fertility Centre, International Centre for Life, Newcastle upon Tyne, England, United Kingdom

John Rhodes

Affiliation Stockton Quality Control Laboratory, University Hospital of North Tees, Stockton-on-Tees, England, United Kingdom

Bruce Watson

Affiliation The Centre for Design Research, School of Design, Northumbria University, Newcastle upon Tyne, England, United Kingdom

Robin Henderson

Affiliation School of Mathematics and Statistics, Herschel Building, Newcastle University, Newcastle upon Tyne, England, United Kingdom

Alison Murdoch

Affiliation Newcastle Fertility Centre, International Centre for Life, Newcastle upon Tyne, England, United Kingdom

Mary Herbert

mary.herbert@ncl.ac.uk

Affiliations Newcastle Fertility Centre, International Centre for Life, Newcastle upon Tyne, England, United Kingdom, Institute for Ageing and Health, Newcastle University, Newcastle Fertility Centre, Centre for Life, Newcastle upon Tyne, England, United Kingdom

Competing Interests

MH, JF, SH are named inventors on the patents: Europe 1940548 A1 (published on 9/7/2008), UK 2433042 (Granted 4/1/2011), US 2010/0291664 (published on 18/11/2010), Canada 2628266(published on 3/5/2007) which is owned by the NHS. Newcastle Fertility Centre receive a percentage of the royalties. There are no products in development to declare. The product is currently marketed under Vitrosafe. This does not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: JF SH BW AM MH. Performed the experiments: LH NP LN JF SH SB JR AM MH. Analyzed the data: LH NP LN RH AM MH. Contributed reagents/materials/analysis tools: JR BW RH. Wrote the paper: LH NP RH AM MH. Revision and final approval of manuscript: LH NP LN JF SH SB JR BW RH AM MH.