An Enhanced Monomeric Blue Fluorescent Protein with the High Chemical Stability of the Chromophore

Oksana M. Subach; Paula J. Cranfill; Michael W. Davidson; Vladislav V. Verkhusha

doi:10.1371/journal.pone.0028674

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Peer-reviewed

Research Article

An Enhanced Monomeric Blue Fluorescent Protein with the High Chemical Stability of the Chromophore

Oksana M. Subach,

Affiliation Department of Anatomy and Structural Biology, and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, New York, New York, United States of America
⨯
Paula J. Cranfill,

Affiliation National High Magnetic Field Laboratory and Department of Biological Science, The Florida State University, Tallahassee, Florida, United States of America
⨯
Michael W. Davidson,

Affiliation National High Magnetic Field Laboratory and Department of Biological Science, The Florida State University, Tallahassee, Florida, United States of America
⨯
Vladislav V. Verkhusha

* E-mail: vladislav.verkhusha@einstein.yu.edu

Affiliation Department of Anatomy and Structural Biology, and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, New York, New York, United States of America
⨯

An Enhanced Monomeric Blue Fluorescent Protein with the High Chemical Stability of the Chromophore

Oksana M. Subach,
Paula J. Cranfill,
Michael W. Davidson,
Vladislav V. Verkhusha

Published: December 8, 2011
https://doi.org/10.1371/journal.pone.0028674

About the Authors

Oksana M. Subach

Affiliation Department of Anatomy and Structural Biology, and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, New York, New York, United States of America

Paula J. Cranfill

Affiliation National High Magnetic Field Laboratory and Department of Biological Science, The Florida State University, Tallahassee, Florida, United States of America

Michael W. Davidson

Affiliation National High Magnetic Field Laboratory and Department of Biological Science, The Florida State University, Tallahassee, Florida, United States of America

Vladislav V. Verkhusha

* E-mail: vladislav.verkhusha@einstein.yu.edu

Affiliation Department of Anatomy and Structural Biology, and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, New York, New York, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: VVV OMS. Wrote the paper: VVV OMS. Developed and characterized purified protein: OMS. Characterized mTagBFP2 in mammalian cells: OMS PJC MWD .

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Chromophores
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Fluorescence resonance energy transfer
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Fluorescence
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Green fluorescent protein
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Blue fluorescent protein
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Cell fusion
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HeLa cells
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Transfection
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