About the Authors

Benjamin M. Woolston

Affiliation Department of Chemical Engineering, Pennsylvania State University, University Park, Pennsylvania, United States of America

Carl Schlagnhaufer

Affiliations Department of Plant Pathology, Pennsylvania State University, University Park, United States of America, Agarigen, Inc., Durham, North Carolina, United States of America

Jack Wilkinson

Affiliation Agarigen, Inc., Durham, North Carolina, United States of America

Jeffrey Larsen

Affiliation Department of Chemical Engineering, Pennsylvania State University, University Park, Pennsylvania, United States of America

Zhixin Shi

Affiliation Agarigen, Inc., Durham, North Carolina, United States of America

Kimberly M. Mayer

Affiliation Agarigen, Inc., Durham, North Carolina, United States of America

Donald S. Walters

Affiliation Agarigen, Inc., Durham, North Carolina, United States of America

Wayne R. Curtis

Affiliation Department of Chemical Engineering, Pennsylvania State University, University Park, Pennsylvania, United States of America

C. Peter Romaine

cpr2@psu.edu

Affiliations Department of Plant Pathology, Pennsylvania State University, University Park, United States of America, Agarigen, Inc., Durham, North Carolina, United States of America

Competing Interests

The authors have read the journal's policy and have the following conflicts: Carl Schlagnhaufer, Jack Wilkinson, Zhixin Shi, Kimberly M. Mayer, Donald S. Walters and C. Peter Romaine are affiliated with Intrexon Corporation, which acquired Agarigen, Inc. and maintains an interest in Agaricus bisporus for recombinant expression. The protein translocation phenomenon described in this publication has been disclosed in a patent application (PCT/US2011/032087) filed by Penn State. This does not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: CPR BMW CS . Performed the experiments: BMW CPR CS ZS. Analyzed the data: BMW CS CPR ZS JW KMM DSW WRC. Contributed reagents/materials/analysis tools: JW ZS JL KMM DSW WRC. Wrote the paper: CPR BMW CS.