About the Authors

George W. Rogers

Affiliation Seahorse Bioscience, North Billerica, Massachusetts, United States of America

Martin D. Brand

Affiliation Buck Institute for Age Research, Novato, California, United States of America

Susanna Petrosyan

Affiliation Department of Pharmacology, University of California San Diego, La Jolla, California, United States of America

Deepthi Ashok

Affiliation Buck Institute for Age Research, Novato, California, United States of America

Alvaro A. Elorza

Current address: Department of Biology, Universidad Andrés Bello, Santiago, Chile

Affiliation Seahorse Bioscience, North Billerica, Massachusetts, United States of America

David A. Ferrick

Affiliation Seahorse Bioscience, North Billerica, Massachusetts, United States of America

Anne N. Murphy

anmurphy@ucsd.edu

Affiliation Department of Pharmacology, University of California San Diego, La Jolla, California, United States of America

Competing Interests

The authors have the following competing interest: George W. Rogers and David A. Ferrick are current employees of Seahorse Bioscience. Dr. Alvaro A. Elorza is a former employee of Seahorse Bioscience. There are no patents, products in development or marketed products to declare. However, Seahorse Bioscience produces a product, the XF24 analyzer, that is used for development of the approach described in this manuscript. This does not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials, as detailed online in the guide for authors.

Author Contributions

Conceived and designed the experiments: GWR MDB AAE DAF ANM. Performed the experiments: GWR MDB SP DA ANM. Analyzed the data: GWR MDB SP DA ANM. Wrote the paper: GWR MDB ANM.