Genotyping of Genetically Monomorphic Bacteria: DNA Sequencing in Mycobacterium tuberculosis Highlights the Limitations of Current Methodologies

Iñaki Comas; Susanne Homolka; Stefan Niemann; Sebastien Gagneux

doi:10.1371/journal.pone.0007815

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Open Access

Peer-reviewed

Research Article

Genotyping of Genetically Monomorphic Bacteria: DNA Sequencing in Mycobacterium tuberculosis Highlights the Limitations of Current Methodologies

Iñaki Comas,

Affiliation Division of Mycobacterial Research, Medical Research Council, National Institute for Medical Research, London, United Kingdom
⨯
Susanne Homolka,

Affiliation Molecular Mycobacteriology, Research Center Borstel, Borstel, Germany
⨯
Stefan Niemann ,

Contributed equally to this work with: Stefan Niemann, Sebastien Gagneux

Affiliation Molecular Mycobacteriology, Research Center Borstel, Borstel, Germany
⨯
Sebastien Gagneux

Contributed equally to this work with: Stefan Niemann, Sebastien Gagneux

* E-mail: gagneux@nimr.mrc.ac.uk

Affiliation Division of Mycobacterial Research, Medical Research Council, National Institute for Medical Research, London, United Kingdom
⨯

Genotyping of Genetically Monomorphic Bacteria: DNA Sequencing in Mycobacterium tuberculosis Highlights the Limitations of Current Methodologies

Iñaki Comas,
Susanne Homolka,
Stefan Niemann,
Sebastien Gagneux

Published: November 12, 2009
https://doi.org/10.1371/journal.pone.0007815

About the Authors

Iñaki Comas

Affiliation Division of Mycobacterial Research, Medical Research Council, National Institute for Medical Research, London, United Kingdom

Susanne Homolka

Affiliation Molecular Mycobacteriology, Research Center Borstel, Borstel, Germany

Stefan Niemann

Contributed equally to this work with: Stefan Niemann, Sebastien Gagneux

Affiliation Molecular Mycobacteriology, Research Center Borstel, Borstel, Germany

Sebastien Gagneux

Contributed equally to this work with: Stefan Niemann, Sebastien Gagneux

* E-mail: gagneux@nimr.mrc.ac.uk

Affiliation Division of Mycobacterial Research, Medical Research Council, National Institute for Medical Research, London, United Kingdom

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: SN SG. Performed the experiments: SH SN SG. Analyzed the data: IC SN SG. Contributed reagents/materials/analysis tools: SN SG. Wrote the paper: IC SN SG.

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Phylogenetics
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Genotyping
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Genetic epidemiology
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Phylogenetic analysis
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Bacterial pathogens
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Microbial genetics
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Molecular epidemiology
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Single nucleotide polymorphisms
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