A Novel Tandem Reporter Quantifies RNA Polymerase II Termination in Mammalian Cells

Ayan Banerjee; Mimi C. Sammarco; Scott Ditch; Jeffrey Wang; Ed Grabczyk

doi:10.1371/journal.pone.0006193

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Open Access

Peer-reviewed

Research Article

A Novel Tandem Reporter Quantifies RNA Polymerase II Termination in Mammalian Cells

Ayan Banerjee ,

Contributed equally to this work with: Ayan Banerjee, Mimi C. Sammarco

Affiliation The Department of Genetics, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America
⨯
Mimi C. Sammarco ,

Contributed equally to this work with: Ayan Banerjee, Mimi C. Sammarco

Affiliation The Department of Genetics, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America
⨯
Scott Ditch,

Affiliation The Department of Genetics, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America
⨯
Jeffrey Wang,

Affiliation The Department of Genetics, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America
⨯
Ed Grabczyk

* E-mail: egrabc@lsuhsc.edu

Affiliation The Department of Genetics, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America
⨯

A Novel Tandem Reporter Quantifies RNA Polymerase II Termination in Mammalian Cells

Ayan Banerjee,
Mimi C. Sammarco,
Scott Ditch,
Jeffrey Wang,
Ed Grabczyk

Published: July 9, 2009
https://doi.org/10.1371/journal.pone.0006193

About the Authors

Ayan Banerjee

Contributed equally to this work with: Ayan Banerjee, Mimi C. Sammarco

Affiliation The Department of Genetics, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America

Mimi C. Sammarco

Contributed equally to this work with: Ayan Banerjee, Mimi C. Sammarco

Affiliation The Department of Genetics, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America

Scott Ditch

Affiliation The Department of Genetics, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America

Jeffrey Wang

Affiliation The Department of Genetics, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America

Ed Grabczyk

* E-mail: egrabc@lsuhsc.edu

Affiliation The Department of Genetics, Louisiana State University Health Sciences Center, New Orleans, Louisiana, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: MCS EG. Performed the experiments: AB MCS SD JW. Analyzed the data: AB MCS EG. Contributed reagents/materials/analysis tools: AB MCS SD JW EG. Wrote the paper: AB MCS EG.

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Transcriptional termination
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Ribozymes
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DNA transcription
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Polymerase chain reaction
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Polyadenylation
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Luciferase
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Messenger RNA
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RNA polymerase
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