Tat-SF1 Is Not Required for Tat Transactivation but Does Regulate the Relative Levels of Unspliced and Spliced HIV-1 RNAs

Heather B. Miller; Kevin O. Saunders; Georgia D. Tomaras; Mariano A. Garcia-Blanco

doi:10.1371/journal.pone.0005710

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Open Access

Peer-reviewed

Research Article

Tat-SF1 Is Not Required for Tat Transactivation but Does Regulate the Relative Levels of Unspliced and Spliced HIV-1 RNAs

Heather B. Miller,

Affiliations Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, United States of America, Center for RNA Biology, Duke University Medical Center, Durham, North Carolina, United States of America
⨯
Kevin O. Saunders,

Affiliations Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, United States of America, Department of Surgery, Duke University Medical Center, Durham, North Carolina, United States of America
⨯
Georgia D. Tomaras,

Affiliations Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, United States of America, Department of Surgery, Duke University Medical Center, Durham, North Carolina, United States of America
⨯
Mariano A. Garcia-Blanco

* E-mail: garci001@mc.duke.edu

Affiliations Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, United States of America, Center for RNA Biology, Duke University Medical Center, Durham, North Carolina, United States of America, Department of Medicine, Duke University Medical Center, Durham, North Carolina, United States of America
⨯

Tat-SF1 Is Not Required for Tat Transactivation but Does Regulate the Relative Levels of Unspliced and Spliced HIV-1 RNAs

Heather B. Miller,
Kevin O. Saunders,
Georgia D. Tomaras,
Mariano A. Garcia-Blanco

Published: May 27, 2009
https://doi.org/10.1371/journal.pone.0005710

About the Authors

Heather B. Miller

Affiliations Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, United States of America, Center for RNA Biology, Duke University Medical Center, Durham, North Carolina, United States of America

Kevin O. Saunders

Affiliations Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, United States of America, Department of Surgery, Duke University Medical Center, Durham, North Carolina, United States of America

Georgia D. Tomaras

Mariano A. Garcia-Blanco

* E-mail: garci001@mc.duke.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: HM KS GT MGB. Performed the experiments: HM KS. Analyzed the data: HM KS GT MGB. Wrote the paper: HM KS GT MGB.

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HIV-1
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Transfection
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Transactivation
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Viral replication
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Luciferase
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HeLa cells
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Tetracyclines
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Polymerase chain reaction
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