The Choice of PCR Primers Has Great Impact on Assessments of Bacterial Community Diversity and Dynamics in a Wastewater Treatment Plant

Nils Johan Fredriksson; Malte Hermansson; Britt-Marie Wilén

doi:10.1371/journal.pone.0076431

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Open Access

Peer-reviewed

Research Article

The Choice of PCR Primers Has Great Impact on Assessments of Bacterial Community Diversity and Dynamics in a Wastewater Treatment Plant

Nils Johan Fredriksson ,

* E-mail: johan.fredriksson@chalmers.se

Affiliation Department of Civil and Environmental Engineering, Water Environment Technology, Chalmers University of Technology, Gothenburg, Sweden
⨯
Malte Hermansson,

Affiliation Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden
⨯
Britt-Marie Wilén

Affiliation Department of Civil and Environmental Engineering, Water Environment Technology, Chalmers University of Technology, Gothenburg, Sweden
⨯

The Choice of PCR Primers Has Great Impact on Assessments of Bacterial Community Diversity and Dynamics in a Wastewater Treatment Plant

Nils Johan Fredriksson,
Malte Hermansson,
Britt-Marie Wilén

Published: October 1, 2013
https://doi.org/10.1371/journal.pone.0076431

About the Authors

Nils Johan Fredriksson

* E-mail: johan.fredriksson@chalmers.se

Affiliation Department of Civil and Environmental Engineering, Water Environment Technology, Chalmers University of Technology, Gothenburg, Sweden

Malte Hermansson

Affiliation Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden

Britt-Marie Wilén

Affiliation Department of Civil and Environmental Engineering, Water Environment Technology, Chalmers University of Technology, Gothenburg, Sweden

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: NJF BMW. Performed the experiments: NJF BMW. Analyzed the data: NJF BMW MH. Contributed reagents/materials/analysis tools: NJF. Wrote the manuscript: NJF. Contributed with critical revisions of the manuscript: BMW MH.

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Sludge
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Ribosomal RNA
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Sequence alignment
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Database searching
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Polymerase chain reaction
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Sequence databases
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Cloning
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Bacteria
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