Table of Contents

December 2011

The enterotoxigenic E. coli (ETEC) are a common cause of diarrheal illness in developing countries where these organisms cause hundreds of thousands of deaths annually. Antigenic variability among known ETEC vaccine targets has prompted searches for additional conserved antigens that could be used to prevent ETEC infections. In this issue, Harris et al. explore the use of a highly conserved class of surface proteins known as autotransporters as potential ETEC vaccine constituents. This composite image shows host cell membrane (green), cellular actin (red), and nucleic acid (bacteria and cellular nuclei, blue).

Image Credit: James M. Fleckenstein, Thomas Wong, Michael Whitt (University of Tennessee Health Sciences Center)


An Interfaith Dialogue on the Neglected Tropical Diseases

Peter J. Hotez, Serap Aksoy

Photo Quiz

Generalized Serpiginous Eruption during Immunosuppressive Treatment for Leprosy Reactive Neuritis

Carlos Gustavo Wambier, Fernanda Britta Maitto Lemos, Mark Aaron Cappel, Fernando Bellissimo-Rodrigues, Norma Tiraboschi Foss


The Emerging Story of Disability Associated with Lymphatic Filariasis: A Critical Review

Lynne Michelle Zeldenryk, Marion Gray, Richard Speare, Susan Gordon, Wayne Melrose

Research Articles

Effect of a Control Project on Clinical Profiles and Outcomes in Buruli Ulcer: A Before/After Study in Bas-Congo, Democratic Republic of Congo

Delphin Mavinga Phanzu, Patrick Suykerbuyk, Désiré Bofunga B. Imposo, Philippe Ngwala Lukanu, Jean-Bedel Masamba Minuku, Linda F. Lehman, Paul Saunderson, Bouke C. de Jong, Pascal Tshindele Lutumba, Françoise Portaels, Marleen Boelaert

Single Dose Novel Salmonella Vaccine Enhances Resistance against Visceralizing L. major and L. donovani Infection in Susceptible BALB/c Mice

Juliane Schroeder, Najmeeyah Brown, Paul Kaye, Toni Aebischer

Individuals with Le(a+b−) Blood Group Have Increased Susceptibility to Symptomatic Vibrio cholerae O1 Infection

Mohammad Arifuzzaman, Tanvir Ahmed, Mohammad Arif Rahman, Fahima Chowdhury, Rasheduzzaman Rashu, Ashraful I. Khan, Regina C. LaRocque, Jason B. Harris, Taufiqur Rahman Bhuiyan, Edward T. Ryan, Stephen B. Calderwood, Firdausi Qadri

Dengue Virus Type 4 Phylogenetics in Brazil 2011: Looking beyond the Veil

Renato Pereira de Souza, Iray M. Rocco, Adriana Y. Maeda, Carine Spenassatto, Ivani Bisordi, Akemi Suzuki, Vivian R. Silveira, Sarai J. S. Silva, Roberta M. Azevedo, Fernanda M. Tolentino, Jaqueline C. Assis, Margarida G. Bassi, Bibiana P. Dambrós, Gabriela L. Tumioto, Tatiana S. Gregianini, Luiza Terezinha M. Souza, Maria do Carmo S. T. Timenetsky, Cecília L. S. Santos

In Vitro and In Vivo Efficacy of Monepantel (AAD 1566) against Laboratory Models of Human Intestinal Nematode Infections

Lucienne Tritten, Angelika Silbereisen, Jennifer Keiser

Genetic Engineering of Trypanosoma (Dutonella) vivax and In Vitro Differentiation under Axenic Conditions

Simon D'Archivio, Mathieu Medina, Alain Cosson, Nathalie Chamond, Brice Rotureau, Paola Minoprio, Sophie Goyard

Population Dynamics of Aedes aegypti and Dengue as Influenced by Weather and Human Behavior in San Juan, Puerto Rico

Roberto Barrera, Manuel Amador, Andrew J. MacKay

Evaluation of Amoebicidal Potential of Paneth Cell Cryptdin-2 against Entamoeba histolytica

Simran Preet, Sanjay Bharati, Geeta Shukla, Ashwani Koul, Praveen Rishi

Tropheryma whipplei: A Common Bacterium in Rural Senegal

Alpha Kabinet Keita, Hubert Bassene, Adama Tall, Cheikh Sokhna, Pavel Ratmanov, Jean-François Trape, Didier Raoult, Florence Fenollar

Tools to Support Policy Decisions Related to Treatment Strategies and Surveillance of Schistosomiasis Japonica towards Elimination

Xiao-Nong Zhou, Jing Xu, Hong-Gen Chen, Tian-Ping Wang, Xi-Bao Huang, Dan-Dan Lin, Qi-Zhi Wang, Li Tang, Jia-Gang Guo, Xiao-Hua Wu, Ting Feng, Jia-Xu Chen, Jian Guo, Shao-Hong Chen, Hao Li, Zhong-Dao Wu, Rosanna W. Peeling

Integrated Dataset of Screening Hits against Multiple Neglected Disease Pathogens

Solomon Nwaka, Dominique Besson, Bernadette Ramirez, Louis Maes, An Matheeussen, Quentin Bickle, Nuha R. Mansour, Fouad Yousif, Simon Townson, Suzanne Gokool, Fidelis Cho-Ngwa, Moses Samje, Shailja Misra-Bhattacharya, P. K. Murthy, Foluke Fakorede, Jean-Marc Paris, Clive Yeates, Robert Ridley, Wesley C. Van Voorhis, Timothy Geary

Activation of the Innate Immune Response against DENV in Normal Non-Transformed Human Fibroblasts

José Bustos-Arriaga, Jazmín García-Machorro, Moisés León-Juárez, Julio García-Cordero, Leopoldo Santos-Argumedo, Leopoldo Flores-Romo, A. René Méndez-Cruz, Francisco J. Juárez-Delgado, Leticia Cedillo-Barrón

An Unexpected Recurrent Transmission of Rift Valley Fever Virus in Cattle in a Temperate and Mountainous Area of Madagascar

Veronique Chevalier, Toky Rakotondrafara, Marion Jourdan, Jean Michel Heraud, Harena Rasamoelina Andriamanivo, Benoit Durand, Julie Ravaomanana, Pierre E. Rollin, René Rakotondravao

Multiplicity and Diversity of Plasmodium vivax Infections in a Highly Endemic Region in Papua New Guinea

Cristian Koepfli, Amanda Ross, Benson Kiniboro, Thomas A. Smith, Peter A. Zimmerman, Peter Siba, Ivo Mueller, Ingrid Felger

Economic Impact of Dengue Illness and the Cost-Effectiveness of Future Vaccination Programs in Singapore

Luis R. Carrasco, Linda K. Lee, Vernon J. Lee, Eng Eong Ooi, Donald S. Shepard, Tun L. Thein, Victor Gan, Alex R. Cook, David Lye, Lee Ching Ng, Yee Sin Leo

Post-Kala-azar Dermal Leishmaniasis in Nepal: A Retrospective Cohort Study (2000–2010)

Surendra Uranw, Bart Ostyn, Arpana Rijal, Saru Devkota, Basudha Khanal, Joris Menten, Marleen Boelaert, Suman Rijal

Novel, Meso-Substituted Cationic Porphyrin Molecule for Photo-Mediated Larval Control of the Dengue Vector Aedes aegypti

Leonardo Lucantoni, Michela Magaraggia, Giulio Lupidi, Robert Kossivi Ouedraogo, Olimpia Coppellotti, Fulvio Esposito, Clara Fabris, Giulio Jori, Annette Habluetzel

Identification of Loci Controlling Restriction of Parasite Growth in Experimental Taenia crassiceps Cysticercosis

Ruben Ramirez-Aquino, Irena Radovanovic, Anny Fortin, Edda Sciutto-Conde, Gladis Fragoso-González, Philippe Gros, Irma Aguilar-Delfin

Cytoplasmic Incompatibility as a Means of Controlling Culex pipiens quinquefasciatus Mosquito in the Islands of the South-Western Indian Ocean

Célestine M. Atyame, Nicole Pasteur, Emilie Dumas, Pablo Tortosa, Michaël Luciano Tantely, Nicolas Pocquet, Séverine Licciardi, Ambicadutt Bheecarry, Betty Zumbo, Mylène Weill, Olivier Duron

Novel Structural Components of the Ventral Disc and Lateral Crest in Giardia intestinalis

Kari D. Hagen, Matthew P. Hirakawa, Susan A. House, Cindi L. Schwartz, Jonathan K. Pham, Michael J. Cipriano, Moises J. De La Torre, Albert C. Sek, Gary Du, Brystal M. Forsythe, Scott C. Dawson

Multifaceted Population Structure and Reproductive Strategy in Leishmania donovani Complex in One Sudanese Village

Virginie Rougeron, Thierry De Meeûs, Mallorie Hide, Georges Le Falher, Bruno Bucheton, Jacques Dereure, Sayda H. El-Safi, Alain Dessein, Anne-Laure Bañuls

IFN-γ Production Depends on IL-12 and IL-18 Combined Action and Mediates Host Resistance to Dengue Virus Infection in a Nitric Oxide-Dependent Manner

Caio T. Fagundes, Vivian V. Costa, Daniel Cisalpino, Flávio A. Amaral, Patrícia R. S. Souza, Rafael S. Souza, Bernhard Ryffel, Leda Q. Vieira, Tarcília A. Silva, Alena Atrasheuskaya, George Ignatyev, Lirlândia P. Sousa, Danielle G. Souza, Mauro M. Teixeira

Metabolic Variation during Development in Culture of Leishmania donovani Promastigotes

Ana Marta Silva, Anabela Cordeiro-da-Silva, Graham H. Coombs

Mosquito Host Selection Varies Seasonally with Host Availability and Mosquito Density

Tara C. Thiemann, Sarah S. Wheeler, Christopher M. Barker, William K. Reisen

High Prevalence of Drug Resistance in Animal Trypanosomes without a History of Drug Exposure

Simbarashe Chitanga, Tanguy Marcotty, Boniface Namangala, Peter Van den Bossche, Jan Van Den Abbeele, Vincent Delespaux

Cytokine and Protein Markers of Leprosy Reactions in Skin and Nerves: Baseline Results for the North Indian INFIR Cohort

Diana N. J. Lockwood, Lavanya Suneetha, Karuna Devi Sagili, Meher Vani Chaduvula, Ismail Mohammed, Wim van Brakel, W. C. Smith, Peter Nicholls, Sujai Suneetha

Disability Weight of Clonorchis sinensis Infection: Captured from Community Study and Model Simulation

Men-Bao Qian, Ying-Dan Chen, Yue-Yi Fang, Long-Qi Xu, Ting-Jun Zhu, Tan Tan, Chang-Hai Zhou, Guo-Fei Wang, Tie-Wu Jia, Guo-Jing Yang, Xiao-Nong Zhou

Epidemiological Tracking and Population Assignment of the Non-Clonal Bacterium, Burkholderia pseudomallei

Julia Dale, Erin P. Price, Heidie Hornstra, Joseph D. Busch, Mark Mayo, Daniel Godoy, Vanaporn Wuthiekanun, Anthony Baker, Jeffrey T. Foster, David M. Wagner, Apichai Tuanyok, Jeffrey Warner, Brian G. Spratt, Sharon J. Peacock, Bart J. Currie, Paul Keim, Talima Pearson

Toward an Open-Access Global Database for Mapping, Control, and Surveillance of Neglected Tropical Diseases

Eveline Hürlimann, Nadine Schur, Konstantina Boutsika, Anna-Sofie Stensgaard, Maiti Laserna de Himpsl, Kathrin Ziegelbauer, Nassor Laizer, Lukas Camenzind, Aurelio Di Pasquale, Uwem F. Ekpo, Christopher Simoonga, Gabriel Mushinge, Christopher F. L. Saarnak, Jürg Utzinger, Thomas K. Kristensen, Penelope Vounatsou

A Deep Sequencing Approach to Comparatively Analyze the Transcriptome of Lifecycle Stages of the Filarial Worm, Brugia malayi

Young-Jun Choi, Elodie Ghedin, Matthew Berriman, Jacqueline McQuillan, Nancy Holroyd, George F. Mayhew, Bruce M. Christensen, Michelle L. Michalski

Tracing of the Bile-Chemotactic Migration of Juvenile Clonorchis sinensis in Rabbits by PET-CT

Tae Im Kim, Won Gi Yoo, Byung Kook Kwak, Ju–Won Seok, Sung–Jong Hong

Epidemiologic Aspects of an Emerging Focus of Visceral Leishmaniasis in Tbilisi, Georgia

Ekaterina Giorgobiani, Nazibrola Chitadze, Gvantsa Chanturya, Marina Grdzelidze, Ryan C. Jochim, Anna Machablishvili, Tsiuri Tushishvili, Yulia Zedginidze, Marina K. Manjgaladze, Nino Iashvili, Manana P. Makharadze, Tsiuri Zakaraya, Konstantin Kikaleishvili, Ivan Markhvashvili, Goderdzi Badashvili, Teymuraz Daraselia, Michael P. Fay, Shaden Kamhawi, David Sacks

Ciguatera Fish Poisoning in the Pacific Islands (1998 to 2008)

Mark P. Skinner, Tom D. Brewer, Ron Johnstone, Lora E. Fleming, Richard J. Lewis

Knockout of the dhfr-ts Gene in Trypanosoma cruzi Generates Attenuated Parasites Able to Confer Protection against a Virulent Challenge

Cecilia Perez Brandan, Angel M. Padilla, Dan Xu, Rick L. Tarleton, Miguel A. Basombrio

In Vivo Expression of Salmonella enterica Serotype Typhi Genes in the Blood of Patients with Typhoid Fever in Bangladesh

Alaullah Sheikh, Richelle C. Charles, Nusrat Sharmeen, Sean M. Rollins, Jason B. Harris, Md. Saruar Bhuiyan, Mohammad Arifuzzaman, Farhana Khanam, Archana Bukka, Anuj Kalsy, Steffen Porwollik, Daniel T. Leung, W. Abdullah Brooks, Regina C. LaRocque, Elizabeth L. Hohmann, Alejandro Cravioto, Tanya Logvinenko, Stephen B. Calderwood, Michael McClelland, James E. Graham, Firdausi Qadri, Edward T. Ryan

A LigA Three-Domain Region Protects Hamsters from Lethal Infection by Leptospira interrogans

Mariana L. Coutinho, Henry A. Choy, Melissa M. Kelley, James Matsunaga, Jane T. Babbitt, Michael S. Lewis, Jose Antonio G. Aleixo, David A. Haake

Novel Insights in the Fecal Egg Count Reduction Test for Monitoring Drug Efficacy against Soil-Transmitted Helminths in Large-Scale Treatment Programs

Bruno Levecke, Niko Speybroeck, Robert J. Dobson, Jozef Vercruysse, Johannes Charlier

Nipah Virus Transmission in a Hamster Model

Emmie de Wit, Trenton Bushmaker, Dana Scott, Heinz Feldmann, Vincent J. Munster

Dynamics of Parasite Clearance in Cutaneous Leishmaniasis Patients Treated with Miltefosine

Thomas P. C. Dorlo, Pieter P. A. M. van Thiel, Gerard J. Schoone, Ymkje Stienstra, Michèle van Vugt, Jos H. Beijnen, Peter J. de Vries

Risk Factors for Ocular Chlamydia after Three Mass Azithromycin Distributions

Berhan Ayele, Teshome Gebre, Jeanne Moncada, Jenafir I. House, Nicole E. Stoller, Zhaoxia Zhou, Travis C. Porco, Bruce D. Gaynor, Paul M. Emerson, Julius Schachter, Jeremy D. Keenan

Examining the Relationship between Urogenital Schistosomiasis and HIV Infection

Pamela Sabina Mbabazi, Olivia Andan, Daniel W. Fitzgerald, Lester Chitsulo, Dirk Engels, Jennifer A. Downs

Utility of the Tourniquet Test and the White Blood Cell Count to Differentiate Dengue among Acute Febrile Illnesses in the Emergency Room

Christopher J. Gregory, Olga D. Lorenzi, Lisandra Colón, Arleene Sepúlveda García, Luis M. Santiago, Ramón Cruz Rivera, Liv Jossette Cuyar Bermúdez, Fernando Ortiz Báez, Delanor Vázquez Aponte, Kay M. Tomashek, Jorge Gutierrez, Luisa Alvarado

Tissue Tropism and Target Cells of NSs-Deleted Rift Valley Fever Virus in Live Immunodeficient Mice

Céline Gommet, Agnès Billecocq, Grégory Jouvion, Milena Hasan, Tânia Zaverucha do Valle, Laurent Guillemot, Charlène Blanchet, Nico van Rooijen, Xavier Montagutelli, Michèle Bouloy, Jean-Jacques Panthier

Directed Evaluation of Enterotoxigenic Escherichia coli Autotransporter Proteins as Putative Vaccine Candidates

Jessica A. Harris, Koushik Roy, Virginia Woo-Rasberry, David J. Hamilton, Rita Kansal, Firdausi Qadri, James M. Fleckenstein