Table of Contents

February 2010

Macrophage migration inhibitory factor (MIF) has emerged as a pivotal mediator of innate immunity and has been shown to be an important effector molecule in severe sepsis. Melioidosis, caused by Burkholderia pseudomallei, is an important cause of community-acquired sepsis in Southeast Asia. Here an immunostain for MIF is shown in the lungs of mice infected with B. pseudomallei. Positive immunostaining for MIF can be seen in the epithelial submucosa, bronchial epithelial cells, and inflammatory cells, most notably of alveolar macrophages (magnification x 10). MIF concentrations are markedly elevated during melioidosis and correlate with poor outcome. During experimental melioidosis MIF impairs antibacterial defense. (See article by Wiersinga et al., doi:10.1371/journal.pntd.0000605).

Image Credit: Sandrine Florquin (Department of Pathology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands).


The Global Health Crisis and Our Nation's Research Universities

Sandeep P. Kishore, Gloria Tavera, Peter J. Hotez

Policy Platform

Reframing Critical Needs in Vector Biology and Management of Vector-Borne Disease

Shirley Luckhart, Steven W. Lindsay, Anthony A. James, Thomas W. Scott

Research Articles

Field Validation of a Transcriptional Assay for the Prediction of Age of Uncaged Aedes aegypti Mosquitoes in Northern Australia

Leon E. Hugo, Peter E. Cook, Petrina H. Johnson, Luke P. Rapley, Brian H. Kay, Peter A. Ryan, Scott A. Ritchie, Scott L. O'Neill

Strategies for Diagnosis and Treatment of Suspected Leptospirosis: A Cost-Benefit Analysis

Yupin Suputtamongkol, Wirichada Pongtavornpinyo, Yoel Lubell, Chuanpit Suttinont, Siriwan Hoontrakul, Kriangsak Phimda, Kitti Losuwanaluk, Duangjai Suwancharoen, Saowaluk Silpasakorn, Wirongrong Chierakul, Nick Day

Asymptomatic Renal Colonization of Humans in the Peruvian Amazon by Leptospira

Christian A. Ganoza, Michael A. Matthias, Mayuko Saito, Manuel Cespedes, Eduardo Gotuzzo, Joseph M. Vinetz

Evaluation of Oxfendazole, Praziquantel and Albendazole against Cystic Echinococcosis: A Randomized Clinical Trial in Naturally Infected Sheep

Cesar M. Gavidia, Armando E. Gonzalez, Eduardo A. Barron, Berenice Ninaquispe, Monica Llamosas, Manuela R. Verastegui, Colin Robinson, Robert H. Gilman

The Feasibility of Canine Rabies Elimination in Africa: Dispelling Doubts with Data

Tiziana Lembo, Katie Hampson, Magai T. Kaare, Eblate Ernest, Darryn Knobel, Rudovick R. Kazwala, Daniel T. Haydon, Sarah Cleaveland

Effect of Control Strategies on Prevalence, Incidence and Re-infection of Clonorchiasis in Endemic Areas of China

Min-Ho Choi, Sue K. Park, Zhimin Li, Zhuo Ji, Gui Yu, Zheng Feng, Longqi Xu, Seung-Yull Cho, Han-Jong Rim, Soon-Hyung Lee, Sung-Tae Hong

Detection of Wuchereria bancrofti L3 Larvae in Mosquitoes: A Reverse Transcriptase PCR Assay Evaluating Infection and Infectivity

Sandra J. Laney, Reda M. R. Ramzy, Hanan H. Helmy, Hoda A. Farid, Ameen A. Ashour, Gary J. Weil, Steven A. Williams

IL-17 Produced during Trypanosoma cruzi Infection Plays a Central Role in Regulating Parasite-Induced Myocarditis

Paulo Marcos da Matta Guedes, Fredy R. S. Gutierrez, Flavia L. Maia, Cristiane M. Milanezi, Grace K. Silva, Wander R. Pavanelli, João S. Silva

Expression and Function of Macrophage Migration Inhibitory Factor (MIF) in Melioidosis

W. Joost Wiersinga, Thierry Calandra, Liesbeth M. Kager, Gerritje J. W. van der Windt, Thierry Roger, Didier le Roy, Sandrine Florquin, Sharon J. Peacock, Fred C. G. J. Sweep, Tom van der Poll

In vitro Induction of Entamoeba histolytica Cyst-like Structures from Trophozoites

Hugo Aguilar-Díaz, Martha Díaz-Gallardo, Juan P. Laclette, Julio C. Carrero

An “In-Depth” Description of the Small Non-coding RNA Population of Schistosoma japonicum Schistosomulum

Zhangxun Wang, Xiangyang Xue, Jun Sun, Rong Luo, Xindong Xu, Yanyan Jiang, Qingfeng Zhang, Weiqing Pan

Schistosoma mansoni Stomatin Like Protein-2 Is Located in the Tegument and Induces Partial Protection against Challenge Infection

Leonardo P. Farias, Fernanda C. Cardoso, Patricia A. Miyasato, Bogar O. Montoya, Cibele A. Tararam, Henrique K. Roffato, Toshie Kawano, Andrea Gazzinelli, Rodrigo Correa-Oliveira, Patricia S. Coulson, R. Alan Wilson, Sérgio C. Oliveira, Luciana C. C. Leite

Temporal Expression of Chemokines Dictates the Hepatic Inflammatory Infiltrate in a Murine Model of Schistosomiasis

Melissa L. Burke, Donald P. McManus, Grant A. Ramm, Mary Duke, Yuesheng Li, Malcolm K. Jones, Geoffrey N. Gobert

Transcriptional Changes in Schistosoma mansoni during Early Schistosomula Development and in the Presence of Erythrocytes

Geoffrey N. Gobert, Mai H. Tran, Luke Moertel, Jason Mulvenna, Malcolm K. Jones, Donald P. McManus, Alex Loukas

Reductions in the Prevalence and Incidence of Geohelminth Infections following a City-wide Sanitation Program in a Brazilian Urban Centre

Luciene Maura Mascarini-Serra, Carlos A. Telles, Matildes S. Prado, Sheila Alvim Mattos, Agostino Strina, Neuza M. Alcantara-Neves, Mauricio L. Barreto

Thrichomys laurentius (Rodentia; Echimyidae) as a Putative Reservoir of Leishmania infantum and L. braziliensis: Patterns of Experimental Infection

André Luiz Rodrigues Roque, Elisa Cupolillo, Renato Sergio Marchevsky, Ana Maria Jansen

Prevalence, Clinical Staging and Risk for Blood-Borne Transmission of Chagas Disease among Latin American Migrants in Geneva, Switzerland

Yves Jackson, Laurent Gétaz, Hans Wolff, Marylise Holst, Anne Mauris, Aglaé Tardin, Juan Sztajzel, Valérie Besse, Louis Loutan, Jean-Michel Gaspoz, Jean Jannin, Pedro Albajar Vinas, Alejandro Luquetti, François Chappuis

Electroporation Facilitates Introduction of Reporter Transgenes and Virions into Schistosome Eggs

Kristine J. Kines, Gabriel Rinaldi, Tunika I. Okatcha, Maria E. Morales, Victoria H. Mann, Jose F. Tort, Paul J. Brindley

Prevalence and Risk Factors of Porcine Cysticercosis in Angónia District, Mozambique

Alberto Pondja, Luís Neves, James Mlangwa, Sónia Afonso, José Fafetine, Arve Lee Willingham III, Stig Milan Thamsborg, Maria Vang Johansen