6 Jan 2016: Damerow S, Hoppe C, Bandini G, Zarnovican P, Buettner FFR, et al. (2016) Correction: Depletion of UDP-Glucose and UDP-Galactose Using a Degron System Leads to Growth Cessation of Leishmania major. PLOS Neglected Tropical Diseases 10(1): e0004372. https://doi.org/10.1371/journal.pntd.0004372 View correction
Interconversion of UDP-glucose (UDP-Glc) and UDP-galactose (UDP-Gal) by the UDP-Glc 4´-epimerase intimately connects the biosynthesis of these two nucleotide sugars. Their de novo biosynthesis involves transformation of glucose-6-phosphate into glucose-1-phosphate by the phosphoglucomutase and subsequent activation into UDP-Glc by the specific UDP-Glc pyrophosphorylase (UGP). Besides UGP, Leishmania parasites express an uncommon UDP-sugar pyrophosphorylase (USP) able to activate both galactose-1-phosphate and glucose-1-phosphate in vitro. Targeted gene deletion of UGP alone was previously shown to principally affect expression of lipophosphoglycan, resulting in a reduced virulence. Since our attempts to delete both UGP and USP failed, deletion of UGP was combined with conditional destabilisation of USP to control the biosynthesis of UDP-Glc and UDP-Gal. Stabilisation of the enzyme produced by a single USP allele was sufficient to maintain the steady-state pools of these two nucleotide sugars and preserve almost normal glycoinositolphospholipids galactosylation, but at the apparent expense of lipophosphoglycan biosynthesis. However, under destabilising conditions, the absence of both UGP and USP resulted in depletion of UDP-Glc and UDP-Gal and led to growth cessation and cell death, suggesting that either or both of these metabolites is/are essential.
Leishmaniases are a set of tropical and sub-tropical diseases caused by protozoan parasites of the genus Leishmania. They affect about 12 million people and cause a high morbidity. Since treatments against all forms of leishmaniasis are limited in number and efficacy, many efforts are made to identify potential drug targets and develop new therapies. Although considerable progress in genetic manipulation of Leishmania parasites have been made, it remains difficult to study molecules or metabolic pathways essential for parasite viability and growth. In the present work, we used a combination of gene deletion and conditional protein destabilization to demonstrate that biosynthesis of the nucleotide sugar UDP-glucose and its derivative UDP-galactose is essential for parasite growth. Addition of a specific ligand to the culture medium of the engineered parasite protected the targeted enzyme from degradation and enabled cell growth and viability. However, removal of the stabilizing compound led to depletion of UDP-glucose and UDP-galactose, growth arrest and cell death. This work thus opens a new possibility for the study of essential proteins.
Citation: Damerow S, Hoppe C, Bandini G, Zarnovican P, Buettner FR, Lüder CGK, et al. (2015) Depletion of UDP-Glucose and UDP-Galactose Using a Degron System Leads to Growth Cessation of Leishmania major. PLoS Negl Trop Dis 9(11): e0004205. https://doi.org/10.1371/journal.pntd.0004205
Editor: Alvaro Acosta-Serrano, Liverpool School of Tropical Medicine, UNITED KINGDOM
Received: July 5, 2015; Accepted: October 11, 2015; Published: November 3, 2015
Copyright: © 2015 Damerow et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Data Availability: All relevant data are within the paper and its Supporting Information files.
Funding: This study was supported by the Deutsche Forschungsgemeinschaft (Grant RO 3591/2-1 to FHR) and Wellcome Trust (Senior Investigator Award 101842 to MAJF). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Leishmaniases are a set of tropical and sub-tropical diseases caused by protozoan parasites of the genus Leishmania and transmitted by the bite of a sandfly. The severity of the diseases depends on parasite species as well as the immune status of the host and ranges from self-healing cutaneous lesions to fatal visceral infections . According to the World Health Organisation, more than 1 million new cases of cutaneous leishmaniasis and at least 30 000 deaths due to visceral leishmaniasis occur annually. Current treatments are far from ideal and the need to develop new treatments against leishmaniasis is generally recognised .
Advances in genetic manipulation of Leishmania parasites has considerably facilitated the characterisation of metabolic processes and molecules important for parasite proliferation or virulence [3,4]. Nevertheless, the study of essential genes remains difficult since knockouts can only be performed if rescue strategies such as nutrient supplementation or ectopic gene copies can be used. Unfortunately, most Leishmania species including L. major and L. donovani lack a functional RNAi pathway . More recently, a system for conditional destabilisation of protein has been described. This original system involves fusion of a mutated FK506 binding protein destabilising domain to the protein of interest and its stabilisation by addition of a specific ligand . However, this system has not yet been applied to essential proteins.
The promastigote stage of Leishmania parasites synthesises a dense surface glycocalyx and secretes proteophosphoglycans (PPGs) that are both essential for its development and survival within the insect vector . The glycocalyx contains various GPI-anchored molecules including glycoproteins, lipophosphoglycans (LPGs), proteophosphoglycans (PPGs) and the abundant glycoinositolphospholipids (GIPLs) [7,8]. These surface and secreted glycoconjugates are rich in galactose and mannose and thus their biosynthesis requires an abundant supply of GDP-α-D-mannose (GDP-Man) and UDP-α-D-galactose (UDP-Gal). Biosynthesis of GDP-Man is required for formation of the glycocalyx  and for biosynthesis of the carbohydrate storage polymer β1,2-mannan . Since these are essential for virulence, the enzymes involved in GDP-mannose biosynthesis are considered as potential drug targets [10–12].
To address the importance of UDP-Gal biosynthesis in Leishmania, we previously targeted the UDP-glucose pyrophosphorylase (EC 18.104.22.168) (UGP) . This enzyme catalyses the transformation of α-D-glucose-1-phosphate (Glc-1P) and UTP into UDP-glucose (UDP-Glc) . UDP-Glc can then be interconverted into UDP-Gal by the UDP-Glc 4´-epimerase. Unexpectedly, although reduced, galactosylation of the glycocalyx was not abolished in the UGP deficient mutant . The residual galactosylation was explained by the discovery of an unusual UDP-sugar pyrophosphorylase (USP) (EC 22.214.171.124) that can activate α-D-galactose-1-phosphate (Gal-1P) and Glc-1P with UTP to form the corresponding UDP-sugar . Recently, deletion of USP in L. major was shown to abolish conversion of Gal-1P into UDP-Gal confirming its role in galactose salvage . Interestingly, the hexose transporters of the related trypanosomatids T. brucei and T. cruzi are unable to transport galactose [17,18] and the only route to UDP-Gal formation in these two parasites is via epimerisation of UDP-Glc, which is essential for parasite growth [19–21].
In this study, we applied a combination of gene deletion and protein destabilisation to evaluate the importance of the UDP-Glc/UDP-Gal biosynthesis. Thus, biosynthesis of these two nucleotide sugars could be reduced to minimal level, leading to growth cessation and cell death.
Promastigote cultures of wild type L. major MHOM/SU/73/5ASKH and respective mutant cell lines were grown at 27°C in standard culture media consisting of M199 medium (Invitrogen) supplemented with 10% heat inactivated fetal calf serum, 40 mM Hepes pH 7.5, 0.1 mM adenine, 0.0005% hemin, 0.0002% biotin. Antibiotics (Invivo Gen) were added as required at a concentration of 5 μg/mL phleomycin, 50 μg/mL Hygromycin, 30 μg/mL puromycin and 100 μg/mL nourseothricin. For the maintenance of the ugp-/-usp-/c mutant described below, 1 μM FK506 (LC laboratories) was added to medium. If lower FK506 concentrations were used, parasites were pelleted and washed twice with medium before being resuspended with the desired FK506 concentration.
Generation of L. major ugp-/-usp-/c strain
Sequence of all primers used can be found in S1 Table. A USP gene (LmjF17.1160) replacement cassette carrying the puromycin resistance gene PAC was constructed by double-joint PCR. 2.3 kb of the 5’-UTR and 1.3 kb of the 3’-UTR were amplified with the primer pair 5UTR_1fw/5UTR_1rev and 3UTR_1fw/ 3UTR_1rev respectively. The PAC gene was amplified with primer pair OL_PACfw/OL_PACrev having 52bp homology to the 5´-UTR (forward primer) or 51bp homology to the 3´-UTR (reverse primer). After fusion of the 3 amplicons, the final cassette was obtained by nested PCR with primer pair 5UTR_3fw/3UTR_3rev and ligated into pYES-NTA vector via Not I restriction sites (plasmid #3612). The deletion cassette was excised with Bbv CI/Xcm I, separated on TAE 0.7% agarose, extracted from gel, ethanol precipitated and dissolved in water at ~ 2 μg/μl. Additionally, a knock-in construct was created based on the generic plasmid pGEM-MCS1-PHLEO-DST IR-ddMCS2 (B6323, kind gift of S. M. Beverley) . The phleomycin resistance gene was first exchanged by the nourseothricin resistance gene SAT using Msc I and Rsr II restriction sites, yielding pGEM-MCS1-SAT-DST IR-ddMCS2 (plasmid #3625). Then 2.0 kb of the vicinal USP 5’UTR were cloned with primer pair SD177/SD178 and inserted upstream of SAT in the Nde I and Spe I sites. Similarly, 1.8 kb from the start of the USP gene were amplified with primers SD175/USP3rev and inserted directly behind the destabilisation domain (dd) via the Bgl II and Sac II sites (plasmid #3628). The knock-in cassette was excised with Nde I/Sac II and purified.
Transfections were performed by electroporation, using the high voltage protocol and cytomix buffer as previously described . ugp-/-usp-/+ (Δugp::BLE/Δugp::HYG/Δusp::PAC/USP) clones were generated by transfection of the USP gene replacement cassette into the L. major ugp-/- mutant (Δugp::BLE/Δugp::HYG) . Likewise ugp-/-usp-/c clones (Δugp::HYG/Δugp::BLE/Δusp::PAC/SAT-FKBPFK506i::USP) were obtained by replacing one USP allele by the knock-in cassette to create ugp-/-usp+/c clones (Δugp::HYG/Δugp::BLE/USP/SAT-FKBPFK506i::USP) and subsequently deleting the remaining USP allele. These clones were recovered on semi-solid plates containing 1% Noble agar, appropriate antibiotics and 1 μMFK506. Analysis of 3 single clones is presented in this manuscript. Correct insertion of the respective gene replacement or knock-in cassette was analysed by PCR and Southern blot. Genomic DNA was isolated from Log phase parasites by phenol/chloroform extraction. Southern blots were performed according to standard methods. DIG-labelled probes were synthesised using the DIG DNA labelling mix from Roche with primer pairs SD1/USP1_rev; SD176/SD21 and SD70/SD71.
Early Log phase promastigote lysates were separated on SDS-PAGE and transferred onto PVDF membranes. Equal protein load was assessed by Coomassie brilliant blue protein staining of an identically loaded SDS-PAGE ran separately. Infrared detection on Li-Cor Odyssey Imager was performed after incubation with monoclonal anti-LPG WIC79.3 antibody (protein G purified from mouse hybridoma cells) and goat anti-mouse IgG IR800 Dye 800 CW (Li-Cor) at dilutions of 1:1,000 and 1:20,000, respectively. L. major USP was detected using a 1:20,000 dilution of polyclonal rabbit anti-serum  and goat anti-rabbit IgG IR800 Dye 800 CW (Li-Cor).
In vitro enzyme assays
Conversion of Gal-1P into UDP-Gal or Glc-1P into UDP-Glc by Log phase promastigotes lysates was measured as previously described . Statistical analyses were performed with GraphPad Prism 4 software (Graph-Pad software Inc., La Jolla, CA) using one-way ANOVA with Tukey multiple comparison post-test.
Quantification of nucleotide sugars
Nucleotide sugar pools of wild type L. major and the ugp-/-usp-/c clones conditionally stabilized (1 μM FK506) and partially destabilized (0.01 μM FK506) were measured by high performance liquid chromatography-electrospray ionisation-tandem mass spectrometry using multiple reaction monitoring as previously described . Each nucleotide sugar peak was integrated, normalized to its internal standard GDP-Glc and to the respective nucleotide sugar standard of known concentration. Medium containing 0.01μM FK506 was preferred for this experiment to reduce cell death. Statistical analyses were performed with GraphPad Prism 4 software using a paired t-test to compare ugp-/-usp-/c grown in high and low FK506 containing medium.
GIPL analysis by MALDI-TOF MS
Log phase promastigotes were extracted in chloroform/ methanol/ water (5:10:4), purified over a C18/ SepPak Plus column (Waters) and dried under a nitrogen stream as described previously . GIPLs (1.6x107 cell equivalents) were dissolved in CHCl3/ MeOH/ H2O (15:30:4), mixed with 6-Aza-2-thiothymidine matrix (5 μg/μl H2O) and spotted on a metal target plate. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry was carried out with a Voyager DE Pro (Applied Biosystems, Foster City, CA) in negative-ion reflector mode over the m/z range 900–2000 with an accelerating voltage of 20 kV and a delay of 150 ns. Final mass spectra represented an average of 6–8 spectra, each of which is acquired from 200 laser shots. Spectra were processed using Data Explorer Software V4.8 applying “Advanced Baseline Correction” and “Noise Removal”.
Analysis of cell viability
Late Log phase promastigotes were washed with PBS and transferred to medium containing 1 μM, 0.05 μM, 0.005 μM FK506 or no FK506 at a cell density of 106 cells/ml. After 3 days, parasites were diluted with fresh medium to 6 x 105 cells/ml and allowed to grow for another 2 days. Parasites were then harvested by centrifugation, washed twice in ice-cold PBS, and resuspended at a density of 5 × 106 parasites/ml. After addition of 5 μl of 7-amino-actinomycin D (7-AAD) solution per 100 μl and incubation for 15 min at room temperature in the dark, 8,000 cells of each sample were analysed by FACS (FACSCalibur).
Statistical analyses were performed with GraphPad Prism 4 software using one-way ANOVA with Tukey multiple comparison post-test.
Generation of a mutant with minimal UDP-Glc/UDP-Gal biosynthesis
Upon deletion of UGP, significant amount of UDP-Glc and UDP-Gal was still synthesized by Leishmania parasites , which is likely due to a partial compensation by USP . We thus anticipated that deletion of both USP and UGP would abrogate biosynthesis of these two nucleotide sugars. In a first step, we were able to replace one allele of USP with a gene encoding resistance to puromycin (PAC) in the previously described ugp- mutant  to generate an heterozygous mutant named ugp-/-usp-/+ (Δugp::BLE/Δugp::HYG/Δusp::PAC/USP). However, despite several attempts, replacement of both alleles of USP in the ugp- mutant remained unsuccessful.
Aiming at reducing the UDP-Glc/UDP-Gal biosynthesis to a minimum, we replaced one of the USP allele by a cassette encoding USP N-terminally fused to a mutated FK506 binding protein (FKBP) destabilizing domain called thereafter ddUSP whereas the second USP allele and UGP alleles were deleted (Fig 1). The degron system enables stabilisation of the protein of interest by addition of FK506 to the culture medium and its conditional destabilisation by removal of FK506 . The resulting conditional mutant (Δugp::HYG/Δugp::BLE/Δusp::PAC/SAT-FKBPFK506i::USP) was analysed by southern blot to confirm correct integration of the deletion and knock-in cassettes (S1 Fig) and was named ugp-/-usp-/c. Three independent clones were selected and analysed in this study.
L. major ugp-/-usp-/c mutant was generated from a UGP deficient mutant  by replacement of a USP allele with a construct encoding USP N-terminally fused to a mutated FK506 binding protein destabilizing domain (ddUSP). This system enables stabilisation of the fusion protein by addition of FK506 to the culture medium and its proteasomal degradation in absence of FK506. The second USP allele was replaced by transfection with a puromycin resistance gene (PAC). BLE, phleomycin resistance gene; HYG, hygromycin resistance gene; SAT, nourseothricin resistance gene.
The absence of UGP had been demonstrated in the parental strain by Western blot using a rabbit polyclonal anti-UGP serum . To confirm the functionality of the degradation system in the different ugp-/-usp-/c clones, we analysed the USP protein level in lysates of Log phase promastigotes using a rabbit polyclonal anti-USP serum . As shown in Fig 2A (upper panel), in presence of 1 μM FK506, ddUSP was clearly detectable at approximately 81 kDa (USP: ~69 kDa; destabilising domain: ~12 kDa) whereas in wild type parasites, native USP migrated at approximately 69 kDa. The amount of ddUSP in the different clones stabilised with 1μM FK506 was estimated to 50–60% of wild type from the signal intensity. As anticipated, at lower FK506 concentrations of 0.05 μM, only traces of ddUSP were still discernible and the enzyme was essentially absent if the FK506 concentration was reduced to 0.005 μM (Fig 2A, upper panel, right). As expected USP level was not affected by the FK506 concentration in wild type parasites (Fig 2A, upper panel, left).
(A) Western blot of Log phase wild type or ugp-/-usp-/c promastigotes lysates probed with an anti-USP antiserum (upper panel) or the anti-LPG monoclonal antibody WIC79.3 (middle panel). Parasites were cultivated in medium containing 1, 0.05, 0.005 µM or no FK506 as indicated. Loading was assessed by Coomassie staining of an identically loaded SDS-Page ran separately (lower panel). (B) In vitro conversion of Glc-1P (black bars) or Gal1P (white bars) into nucleotide sugars by cell lysates of wild type and ugp-/-usp-/c mutant cultivated in medium containing various FK506 concentrations as indicated. Values represent the mean ± SEM from n = 3 independent cultures; for the ugp-/-usp-/c mutant, each culture represents a different clone. Significant difference (*p < 0.01), One-way ANOVA with Tukey post test. (C) Negative-ion MALDI spectra of glycosylinositolphospholipids (GIPLs) isolated from wild type and ugp-/-usp-/c mutant grown in presence of 1 or 0.01 μM FK506. Each prominent peak is annotated with its m/z value, the letter a, b, c, d or e referring to the structure depicted above. The lipid moiety consists of a 1-alkyl-2-lyso-phosphatidylinositol or 1-alkyl-2-acyl-phosphatidylinositol. The length of the alkyl and acyl chains is indicated above each peak; C- indicates absence of an alkyl chain.
Regulation of the UDP-Glc/UDP-Gal biosynthesis in L. major using the degron system
The ability of wild type and mutant parasites to convert Glc-1P and Gal-1P into the corresponding nucleotide sugars was measured in promastigote lysates and is presented in Fig 2B. Enzymatic activities measured with lysates of wild type parasites were unaffected by FK506 addition to the growth medium.
When using Glc-1P as substrate, lysate of the ugp-/-usp-/c mutant grown with 1 μM FK506 presented about 4.5% of the activity obtained with wild type lysate. Since the contribution of USP was previously estimated of about 10% in a UGP deficient mutant , the present value is consistent with stabilisation of ddUSP produced by one allele. Only 1% activity could still be measured when the enzyme was stabilised with 0.05 μM FK506 and this dropped below the limits of detection when lower drug concentrations were used.
Similar values were obtained when Gal-1P was used as substrate. With 1μM FK506 in the culture medium, the stabilised ddUSP had an activity of 0.06 U/mg that represents approximately 55% of the wild type activity (0.11 U/mg). When grown in presence of 0.05 μM FK506, the ugp-/-usp-/c mutant lysate showed a low UDP-Gal synthesis capacity of 13% of wild type activity. The enzymatic activity became undetectable with lower drug concentrations.
These results provide evidence for the functionality of the conditional degron system, which allows us to reduce USP protein level and enzymatic activity to background level.
Analysis of LPG and GIPLs
We analysed the effects of regulating UDP-Glc and UDP-Gal biosynthesis, using the degron system, on the production of LPG and GIPLs. Equal amounts of cell lysate were run on 12% SDS-PAGE, blotted on PVDF membrane and developed using the monoclonal antibody WIC79.3, which is specific for galactosylated phosphoglycans repeat units (Fig 2A, middle panel). Loading was controlled by Coomassie blue staining (Figs 2A, lower panel, and S2). As expected, the ugp-/-usp-/c mutant stabilised by the presence of 1 μM FK506 only showed a faint LPG signal, weaker as the signal of the ugp-/- parental strain (S3 Fig). This faint LPG signal (Figs 2A and S3 show the clone with the highest LPG signal) was reduced to below the limits of detection if the mutant was supplemented with lower FK506 concentrations (Fig 2A).
Interestingly, our first attempt to generate an ugp-/-usp-/c mutant resulted in clones devoid of LPG. However, ectopic expression of UGP in these clones or in the ugp-/-usp-/+ clone from which they were derived did not restore LPG biosynthesis. This suggests that Leishmania can adapt to the low UDP-Glc/UDP-Gal availability by shutting down LPG biosynthesis, which is dispensable for in vitro growth [24,25]. In order to avoid in vitro adaptation, the clones presented in this study were maintained in 1 μM FK506 and the number of passages limited. Moreover, LPG expression was regularly checked.
GIPLs purified from the ugp-/-usp-/c mutant and wild type parasites were analysed by MALDI-TOF mass spectrometry in negative ion mode (Figs 2C and S3). The observed ions were annotated based on structures previously reported and correspond to type 2 GIPLs with 0, 1, 2 or 3 galactose residues termed iM2, GIPL-1, GIPL-2 and GIPL-3 respectively . Additional heterogeneity arises from the lipid part that is either an alkylacylglycerol or lysoalkylglycerol with aliphatic chains of various lengths (S2 Table). Remarkably, stabilisation of ddUSP with 1 μM FK506 was sufficient to maintain galactosylation of GIPLs (Figs 2C and S3). However, at low FK506 concentration, GIPLs were virtually agalactosylated. Only traces of GIPL-1 (containing one galactofuranose residue) were still visible at a FK506 concentration of 0.01 μM. This loss of galactosylation upon ddUSP destabilisation reflects depletion of the cell’s UDP-Glc and UDP-Gal pools as shown below.
The nucleotide sugar pools in wild type L. major and the ugp-/-usp-/c clones were measured by high performance liquid chromatography-electrospray ionisation-tandem mass spectrometry using multiple reactions monitoring as previously described . Surprisingly, stabilisation of the enzyme produced from a single ddUSP allele (with 1 μM FK506) was sufficient to maintain the steady-state pools of UDP-Glc and UDP-Gal (Fig 3). In all clones, a strong reduction of the UDP-Glc and UDP-Gal pools was nevertheless observed upon destabilisation of USP in medium containing 0.01 μM FK506. In contrast, the GDP-Man and GDP-Ara pools did not significantly differ from the pools measured in wild type parasites (Fig 3). Unexpectedly, the pool of UDP-N-acetylglucosamine (UDP-GlcNAc) seemed to slightly decrease in the ugp-/-usp-/c mutant upon USP destabilisation (0.01μM FK506) whereas the GDP-fucose (GDP-Fuc) pool, which is very small in wild type parasite, was strongly elevated in these conditions.
Nucleotide sugars were extracted from mid Log phase wild type (wt) and ugp-/-usp-/c promastigotes grown in presence of 1 μM (+) or 0.01 μM (-) FK506 and measured by liquid chromatography-electrospray ionisation-tandem mass spectrometry with multiple reaction monitoring. Values represent the mean ± SD from n = 3 independent cultures; for the ugp-/-usp-/c mutant, each culture represents a different clone. Significant differences (*p < 0.01, ** p < 0.005, *** p < 0.001), paired t-test.
Analysis of growth and cell death in L. major ugp-/-usp-/c mutant
Wild type parasites and the ugp-/-usp-/c mutant maintained in 1 μM FK506 were washed and transferred to medium with various FK506 concentrations at a density of 105 parasites / mL. As seen in Fig 4A (upper panel), growth of the ugp-/-usp-/c mutant slowed down with decreasing FK506 concentrations. If parasites were split after 3 to 4 days (when density reached ~2–3 x 107 cells/mL), the cells continued to grow with kinetics similar to wild type in the presence of 1 μM FK506, whereas no proliferation was observed if FK506 was absent or present at a concentration of 0.005 μM (Fig 4A, lower panel).
(A) In vitro growth of wild type and ugp-/-usp-/c promastigotes grown in absence or presence of various FK506 concentrations as indicated. Medium was inoculated with 105 cells (upper panel). After 3 to 4 days, parasites were transferred to fresh medium containing the indicated FK506 concentration (lower panel). Error bars indicates the standard deviation. (B) Analysis of cell viability. Parasites grown in medium containing the indicated FK506 concentration were labelled with 7-AAD and analysed by flow cytometry. Mean percentages ± SEM of dead parasites (7-AAD+) were calculated from four independent experiments (n = 4). Significant difference (*p < 0.01, *** p < 0.001), One-way ANOVA with Tukey post test.
To analyse cell viability, we labelled parasites grown in medium containing different FK506 concentrations with the DNA intercalator 7-AAD and analysed them by flow cytometry. The results displayed in Fig 4B indicated that less than 5% dead cells were present in cultures of wild type Leishmania. The number of dead cells remained low in the ugp-/-usp-/+ heterozygote or ugp-/-usp-/c cultures supplemented with 0.05μM FK506 or more. In contrast, with a FK506 concentration of 0.005μM or less, the percentage of dead cells exceeded 35%.
In eukaryotes, the biosynthesis of glycans, such as those creating a protective cell surface glycocalyx or cell wall, requires the activation of monosaccharides in the form of nucleotide sugars. These high-energy compounds may be formed from appropriate monosaccharides or be generated from interconversion of existing nucleotide sugars. For example, depending on the cell type, UDP-Gal, UDP-galacturonic acid, UDP-glucuronic acid, UDP-xylose and UDP-arabinose may be generated from UDP-Glc. In Leishmania parasites, however, the variety of nucleotide sugars synthesised is limited to UDP-Glc, UDP-Gal, UDP-GlcNAc, GDP-Man, GDP-Fuc and GDP-arabinose . Previous works have shown that in this protozoan parasite, UDP-Gal is synthesised by a de novo pathway involving UGP [13,14] and by a salvage pathway mediated by USP [15,16]. Deficiency of UGP alone does not abolish biosynthesis of UDP-Gal and its immediate precursor UDP-Glc  since, as we demonstrate here, their biosynthesis is partially compensated by USP. Targeted deletion of both UGP and USP was however unsuccessful suggesting that the metabolites UDP-Glc and/or UDP-Gal are essential for Leishmania in vitro growth. To demonstrate the importance of the UDP-Glc/UDP-Gal biosynthesis, we therefore deleted one of the two USP alleles in the UGP deficient strain (ugp-/-) and used a degron system enabling conditional degradation of the enzyme produced by the remaining USP allele (Fig 1). Addition of 1μM FK506 to the growth medium enabled stabilisation of the ddUSP protein product and supported restricted UDP-Glc and UDP-Gal biosynthesis, which was nonetheless sufficient to maintain parasite growth. With low FK506 concentrations, ddUSP was destabilised and the UDP-Glc/UDP-Gal biosynthesis could be reduced to minimal level leading to growth arrest and cell death, allowing us to demonstrate that UDP-Gal and/or UDP-Glc are essential metabolites for L. major promastigotes.
One of the most distinctive roles of UDP-Glc and derived nucleotide sugars is the biosynthesis of polysaccharides and glycoconjugates forming the cell surface coat. In bacteria, UGP controls the biosynthesis of the most important virulence factors and is valued as antibacterial target since it is frequently essential for pathogenicity or growth . Similarly, in the trypanosomatid parasites Trypanosoma brucei and Trypanosoma cruzi, which are closely related to Leishmania, biosynthesis of UDP-Glc is essential for parasite growth. In these parasites, however, targeting of the UDP-glucose 4´-epimerase demonstrated that the lack of UDP-Gal, rather than its precursor UDP-Glc, is responsible for growth cessation. In both parasites, the growth phenotype was linked to alterations of the glycocalyx [19–21]. In Leishmania, stabilisation of the enzyme produced from a single USP allele with 1 μM FK506 was sufficient to maintain the steady-state UDP-Gal and UDP-Glc pools and support in vitro growth. The maintenance of normal UDP-Gal and UDP-Glc levels under these conditions (where de novo synthesis of UDP-Glc is less than 5% of wild-type) reflects a strong reduction of UDP-Gal consumption at the expense of LPG synthesis. An irreversible shutdown of LPG biosynthesis has even been observed in a heterozygote ugp-/-usp-/+ clone, in adaptation to the inadequate UDP-Gal availability. In contrast, the limited UDP-Gal biosynthesis governed by a single USP allele was sufficient to maintain galactosylation of GIPLs. This is very similar to the situation in T.cruzi epimastigotes, where deletion of one copy of the UDP-glucose 4´-epimerase encoding gene was permissive to the synthesis of galactosylated GIPLs at the expense of the synthesis of the highly galactosylated GPI-anchored surface mucins . One might assume that galactosylated GIPLs are important for in vitro survival and thus are retained by L. major promastigotes. However, a mutant expressing agalactosylated GIPLs due to absence of UDP-galactopyranose mutase (the enzyme that enables formation of UDP-galactofuranose necessary for addition of the first galactose residue) grew normally . In fact, many Leishmania mutants deficient in one or several glycoconjugates have been generated and do not present major in vitro growth phenotypes . Essentiality of the major glycoconjugates such as LPGs, PPGs, GIPLs or N-glycans can therefore be excluded.
Yet, minor components that have until now remained undetected may be crucial for parasite survival. In this regard, we know that the synthesis of GDP-Fuc is essential for T. brucei and likely also for Leishmania [28,29], although to date no fucose containing glycoconjugates have been described in these parasites. Leishmania possesses a unique GDP-Fucose biosynthetic pathway involving two bifunctional enzymes with kinase and pyrophosphorylase activities (encoded by LmJF16.0440 and LmJF16.0480) able to activate L-Fuc into GDP-Fuc. In this parasite, the classical de novo synthesis of GDP-Fuc from GDP-Man seems to be absent. Indeed, deletion of both fucokinase/pyrophosphorylases could only be obtained after expression of the de novo GDP-Fuc pathway enzymes from Trypanosoma . In Trypanosoma brucei, a fucosyltransferase acting on a Gal acceptor structure has recently been characterised  and an orthologue is predicted in Leishmania. The increase of GDP-Fuc observed in the destabilised ugp-/-usp-/c mutant may thus reflect a non-utilisation of this nucleotide sugar due to the absence of acceptor structures. Thus it cannot be excluded that the phenotype observed in the ugp-/-usp-/c mutant is linked to absence of fucose containing structure(s).
As in other eukaryotes, UDP-Glc is also required in the endoplasmic reticulum (ER) for glucosylation of newly synthesised glycoproteins by the UDP-glucose:glycoprotein glucosyltransferase (UGGT); a reaction that promotes protein folding. However, in the trypanosomatids T. brucei and T. cruzi, UGGT is not essential for parasite growth in standard conditions [31,32]. Shortage of UDP-Glc in the ER might thus challenge the protein folding machinery and activates the unfolded protein response [31,33–35] but these are likely not responsible for the lethal phenotype observed under standard culture conditions.
Like other kinetoplastid flagellates, Leishmania parasites also need UDP-Glc in the nucleus for the synthesis of an unusual DNA base called base J (β-D-glucosyl-hydroxymethyluracil) . Base J replaces at most 1% of thymidines in telomeric repeats and at transcription initiation and termination sites and was shown to regulate transcription . In Leishmania, base J is believed to be essential since attempts to delete JBP1, a gene encoding one of the two thymidine hydroxylases involved in the first step of base J synthesis (formation of 5- hydroxymethyluracil), have been unsuccessful . Whether glucosylation of 5-hydroxymethyluracil, is also essential for Leishmania growth remains to be determined. A gradual loss of residual base J upon cell division  could however explain the late appearance of the lethal phenotype of L. major ugp-/-usp-/c.
Further studies are necessary to define whether UDP-Glc and/or UDP-Gal are/is the essential metabolite in Leishmania and to identify the downstream pathway(s) essential for parasite survival. In the trypanosomatids T. brucei and T. cruzi, both the UDP-glucose 4´-epimerase and UGP have been validated as potential therapeutic targets against trypanosomiasis [19–21,39]. However because of the high degree of similarity within the catalytic site with human UGP, specific inhibition of trypanosomatids UGPs might require the design of allosteric inhibitors [39–41]. In Leishmania, the existence of a salvage pathway mediated by USP, which should also be inhibited, is a further challenge for the development of specific drugs. Identification of the essential pathway(s) requiring UDP-Glc or UDP-Gal might thus provide new targets for the development of specific drugs to combat leishmaniases.
S1 Table. Sequence of primers used in this study.
S2 Table. Lipid moieties of Leishmania major GIPLs.
S1 Fig. Southern blot analysis of L. major ugp-/-usp-/c mutant.
(A-C) Genomic DNA from wild type (wt) and homozygous ugp-/-usp-/c mutant (Δugp::HYG/Δugp::BLE/Δusp::PAC/SAT-FKBPFK506i::USP) was digested with four different endonucleases, separated on agarose gel, blotted onto nylon membrane, and hybridized with digoxigenin labeled probes USP*, 5’UTR*, PAC*. The shifts of the probe labeled fragments can be assigned to the respective theoretical length in panel (D-F), indicating correct gene replacement.
The framed area is presented in Fig 2A.
S3 Fig. Comparison of the glycoconjugates synthesised by ugp-/- and stabilised ugp-/-usp-/c mutant.
(A) Western blot of Log phase wild type, ugp-/- and stabilised ugp-/-usp-/c (medium containing 1µM FK506) promastigotes lysates probed with the anti-LPG monoclonal antibody WIC79.3 (upper panel). Loading was assessed by Coomassie staining of an identically loaded SDS-Page ran separately (lower panel). (B) Negative-ion MALDI spectra of glycosylinositolphospholipids (GIPLs) isolated from wild type, ugp-/-, ugp-/-usp-/+ and stabilised ugp-/-usp-/c (grown in medium containing 1µM FK506) promastigotes. Each prominent peak is annotated with the letter a, b, c or d referring to the structure depicted under. The m/z value of prominent peaks has been indicated in the top spectrum. The lipid moiety consists of a 1-alkyl-2-lyso-phosphatidylinositol or 1-alkyl-2-acyl-phosphatidylinositol. The presence and length of the alkyl and acyl chains is indicated in S2 Table.
Conceived and designed the experiments: SD CH GB FRB CGKL MAJF FHR. Performed the experiments: SD CH GB PZ FRB CGKL MAJF FHR. Analyzed the data: SD CH GB FRB CGKL MAJF FHR. Wrote the paper: SD CH MAJF FHR.
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