Figures
All errors found in this paper are due to six samples included in the paper incorrectly assigned as being from Queensland, Australia. The report of Strongyloides fuelleborni infections from Australia made in this paper was incorrect, as those samples in fact originated in Guinea-Bissau and Senegal.
There is an error in Table 4. Specimen Human 333_Au from Queensland (Australia) should be listed as Human 333_GuBi from Guinea-Bissau. Specimen Human 368_16_Au from Queensland (Australia) should be listed as Human 368_16_Se from Senegal. Specimen Human 378_Au from Queensland (Australia) should be listed as Human 378_Bo from Bolivia. Specimen Human 507_Au from Queensland (Australia) should be listed as Human 507_Ni from Nigeria. Specimen Human 524_Au from Queensland (Australia) should be listed as Human 524_Ni from Nigeria. Specimen Human 563_Au from Queensland (Australia) should be listed as Human 563_GuBi from Guinea-Bissau. The authors have provided a corrected Table 4 with the corrected specimens and locations in red.
Fig 1 is incorrect due to the error in Table 4. The authors have provided a corrected version here.
A graphical representation of the Strongyloides spp. genotyping scheme described previously [21], expanded to include additional genotypes from S. stercoralis and S. fuelleborni. This scheme includes novel sequences identified by Barratt et al., and 18S sequences that were available in GenBank (GB), where the appropriate 18S HVR-I and/or HVR-IV regions were captured. Sequences from GB possessing Ns or ambiguous bases were excluded from the scheme. For additional details on the hosts in which these Strongyloides spp. haplotypes were detected refer to the Tables in Barratt et al.
Fig 2 is incorrect due to the error in Table 4. The authors have provided a corrected version here.
This dendrogram represents 787 cox1 sequences, including those generated by Barratt et al. (branches tipped in a black dot) and all published cox1 sequences from GB (at the time this figure was originally prepared) that overlap completely with our 217 base cox1 amplicon (to our knowledge). Peripheral bars are colored according to their site of origin, which corresponds to the colored countries on the map. Branches are color coded separately, according to their identity; either a species assignment, a genus, or their S. stercoralis genotype. The dog image with a black star indicates a sequence from an Australian dog generated by us previously [21], that is distinct from other Strongyloides spp. and clusters between the S. stercoralis and S. fuelleborni groups. The dog image with a black circle highlights a published sequence [21] that clusters close to, yet is distinct from Strongyloides spp. detected previously in lorises [27]. Animal images reflect the mammalian hosts that the sequences were associated with. Two sequences of Strongyloides planiceps (orange branches) from Japanese raccoon dogs serve as an outgroup. The identity of each sequence is provided in S1 Fig which is a searchable PDF of the same dendrogram with all GB accession numbers, the countries of origin, and host species provided. The GB accession numbers for sequences in this dendrogram that were generated as part of this study (branches tipped in a black dot) are provided in S1 File. The sequences used to construct this dendrogram are provided in S2 File. This figure incorporates all corrections detailed in Table 4.
Fig 3 is incorrect due to the error in Table 4. The authors have provided a corrected version here.
Histogram bars are colored according to their origin, corresponding to the colors on the map. A dash in the horizontal axis labels (-) indicates a missing 18S haplotype (either HVR-I or HVR-IV). The colored branches below the horizontal axis correspond to the colored branches in Fig 2, where the red clade represents S. stercoralis types infecting both dogs and humans (lineage A), the green/purple clade represents S. stercoralis types infecting only dogs (lineage B), the gray clade represents S. fuelleborni types from African great apes and humans, the light pink clade represents S. fuelleborni from a baboon and the magenta/light blue clade represents types found in humans and macaques. The absence of a colored branch under a given group indicates that an associated cox1 sequence is presently unavailable for these 18S haplotypes. Black star: A cox1 sequence is not available for these specific worms yet they were assigned to their most appropriate cox1 clades based on the observation that their 18S types have only ever been found previously in worms with cox1 sequences belonging to these clades. Red circle: A novel 18S HVR-IV type without a corresponding cox1 sequence available. As this 18S type had not been described previously, its association with any particular cox1 clade is unknown and cannot be inferred. Green circles: Strongyloides fuelleborni 18S types without corresponding cox1 sequences available. As cox1 sequence data has not been provided for worms with these 18S types, their association with any particular cox1 clade is unknown. Blue circles: Strongyloides fuelleborni 18S types from Japanese macaques. Corresponding cox1 sequences are not available for these specific worms, yet they were assigned to the magenta/light blue clades because all known cox1 sequences from S. fuelleborni infecting Japanese macaques have belonged to this group. This figure incorporates the corrections detailed in Table 4.
Fig 4 is incorrect due to the error in Table 4. The authors have provided a corrected version here.
This figure demonstrates that the cox1 assay described here is broadly specific for Strongyloides spp. and some strongylids. Fragments of cox1 from Ancylostoma spp., Oesophogostomum and Necator americanus have been amplified and sequenced using this assay. Additionally, we previously detected cox1 sequences from Metastrongylus sp., a rotifer, and some unknown nematodes using this approach [21]. The sequences generated in this study are shaded in colors according to their country of origin and those generated by us in a previous study are marked with a black dot on the associated branch tip. The sequences used to construct this dendrogram are included in S3 File. This figure incorporates the corrections detailed in Table 4.
S1 Fig is incorrect due to the errors in the Table 4. The authors have provided a corrected version here.
S1 File is incorrect due to the errors in the Table 4. The authors have provided a corrected version here.
S2 File is incorrect due to the errors in the Table 4. The authors have provided a corrected version here.
S3 File is incorrect due to the errors in Table 4. The authors have provided a corrected version here.
Supporting information
S1 Fig. Cluster dendrogram of cox1 sequences with GB accessions numbers provided.
This file serves as an aid for Fig 1 as it shows an identical dendrogram to Fig 1, yet also provides all GB accession numbers for the sequences included, along with the country of origin and host species. The file allows zooming without loss of resolution, and is also searchable for readers who wish to search the position of specific accession numbers using the ‘Find’ function in Adobe Acrobat PDF reader or another preferred PDF reader. This dendrogram incorporates the corrections detailed in Table 4.
https://doi.org/10.1371/journal.pntd.0009538.s001
(PDF)
S1 File. Excel spreadsheet containing BioSample numbers and GenBank accession numbers.
The details in this supplementary file have been updated in accordance with the corrections in Table 4. All updates are shown in red text.
https://doi.org/10.1371/journal.pntd.0009538.s002
(XLSX)
S2 File. Fasta file of sequences used to construct Fig 2.
Some of the fasta sequence headers in this file were updated according to the corrections detailed in Table 4.
https://doi.org/10.1371/journal.pntd.0009538.s003
(FASTA)
S3 File. Fasta file of sequences used to construct Fig 4.
Some of the fasta sequence headers in this file were updated according to the corrections detailed in Table 4.
https://doi.org/10.1371/journal.pntd.0009538.s004
(FASTA)
Reference
- 1. Barratt JLN, Lane M, Talundzic E, Richins T, Robertson G, Formenti F, et al. (2019) A global genotyping survey of Strongyloides stercoralis and Strongyloides fuelleborni using deep amplicon sequencing. PLoS Negl Trop Dis 13(9): e0007609. https://doi.org/10.1371/journal.pntd.0007609 pmid:31525192
Citation: Barratt JLN, Lane M, Talundzic E, Richins T, Robertson G, Formenti F, et al. (2021) Correction: A global genotyping survey of Strongyloides stercoralis and Strongyloides fuelleborni using deep amplicon sequencing. PLoS Negl Trop Dis 15(6): e0009538. https://doi.org/10.1371/journal.pntd.0009538
Published: June 18, 2021
Copyright: © 2021 Barratt et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.