Multiple vector-borne pathogens of domestic animals in Egypt

Vector Borne Diseases (VBDs) are considered emerging and re-emerging diseases that represent a global burden. The aim of this study was to explore and characterize vector-borne pathogens in different domestic animal hosts in Egypt. A total of 557 blood samples were collected from different animals using a convenience sampling strategy (203 dogs, 149 camels, 88 cattle, 26 buffaloes, 58 sheep and 33 goats). All samples were tested for multiple pathogens using quantitative PCR and standard PCR coupled with sequencing. We identified Theileria annulata and Babesia bigemina in cattle (15.9 and 1.1%, respectively), T. ovis in sheep and buffaloes (8.6 and 7.7%, respectively) and Ba. canis in dogs (0.5%) as well as Anaplasma marginale in cattle, sheep and camels (20.4, 3.4 and 0.7%, respectively) and Coxiella burnetii in sheep and goats (1.7 and 3%; respectively). New genotypes of An. centrale, An. ovis, An. platys-like and Borrelia theileri were found in cattle (1.1,3.4, 3.4 and 3.4%, respectively), An. platys-like in buffaloes (7.7%), An. marginale, An. ovis, An. platys-like and Bo. theileri in sheep (3.4, 1.7, 1.7 and 3.4%, respectively), An. platys, An. platys-like and Setaria digitata in camels (0.7, 5.4 and 0.7%, respectively) and Rickettsia africae-like, An. platys, Dirofilaria repens and Acanthocheilonema reconditum in dogs (1.5, 3.4, 1 and 0.5%, respectively). Co-infections were found in cattle, sheep and dogs (5.7, 1.7, 0.5%, respectively). For the first time, we have demonstrated the presence of several vector-borne zoonoses in the blood of domestic animals in Egypt. Dogs and ruminants seem to play a significant role in the epidemiological cycle of VBDs.

Introduction Infection, the National Research Agency under the program "Investissements d'avenir", reference ANR-10-IAHU-03, the Région Provence Alpes Côte d'Azur and European funding FEDER PRIMI. The authors acknowledge funding from the Science and Technology Development Fund (STDF) and Institut Francais d'Egypte (IFE) (ID: 30652) for the support of this research. The funders just supported the study through chemicals availability. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Borrelioses are zoonotic infectious diseases and are divided into two groups: Lyme disease group (caused by Borrelia burgdorferi and related species) and relapsing fever group [56]. Relapsing fever borrelioses are arthropod-borne spirochetal diseases, usually transmitted by soft ticks; they are common in subtropical regions worldwide [57]. In Africa, relapsing fever is most common in the northern hemisphere and is caused by various Borrelia spp. such as Bo. hispanica, Bo. duttonii, and Bo. crocidurae [57][58][59][60]. Bo. theileri is the etiological agent of bovine borreliosis in ruminants, which causes anemia and fever and, unlike other members of the relapsing fever spirochetes, is transmitted by hard ticks [58]. In Egypt, data on borrelioses in animal hosts are sparse. Only the few studies have detected Bo. burgdorferi [61,62] and Bo. theileri in hard ticks [62].
Q fever is a zoonosis that infects humans and animals through direct contact or a tick bite [63]. Coxiella burnetii is the causative agent of Q fever that may be severe in humans [64]. Infection in animals it is usually subclinical except that reproductive diminution and abortions may occur [65]. Coxiella burnetii infects a wide range of animals, especially sheep, goats, cattle and camels, which serve as reservoirs [64,66]. In Egypt, the seroprevalence of C. burnetii was estimated in buffaloes, sheep, cattle and camels [67][68][69][70]. In addition, C. burnetii has been detected molecularly in goats, camels and ticks (H. dromedarii) [70][71][72].
Filarial nematodes are vector-borne helminths belonging to the order Spiruridae, suborder Spirurina and families Filariidae and Onchocercidae and pose a serious threat to humans and livestock [73,74]. Dirofilaria repens and D. immitis, followed by Acanthocheilonema sp. are the most important etiological agents of filarial infections in dogs [9,73,75]. Setaria digitata is a filarial nematode of cattle and buffaloes and is not pathogenic to these natural hosts, but when transmitted by mosquitoes to accidental hosts such as camels and horses, it can have serious pathological effects [76,77]. In Egypt, information on filarial infections in ruminants and dogs are scarce. In Africa, there are some reports of filarial infections in different places of the continent [78][79][80].
Diagnosis of all these diseases is challenging due to the non-specific febrile illness, difficulty in isolation and cross reactivity of serological methods [35,59]. Therefore, the advanced molecular techniques have been used to increase the sensitivity and specificity of diagnosis, to detect previously unknown pathogens and distinguish closely related species [5]. In Egypt, the epidemiology and prevalence of these diseases remain neglected and poorly understood. To date, few studies have been conducted on individual VBDs in vectors or animal hosts. Here, we provide the first data for molecular screening and characterization of multiple vector-borne pathogens in different animal hosts to better understand the epidemiological approach of VBDs in Egypt.

Ethical approval
This study was approved by the Medical Research Ethics Committee at the National Research Centre, Egypt with the number 19058.

Study area and samples collection
We conducted a cross-sectional observational study with a total of 557 apparently healthy domestic animals (203 dogs, 149 camels, 88 cattle, 26 buffaloes, 58 sheep and 33 goats) using a convenience sampling strategy [81]. Animal blood samples were randomly collected from different provinces in Egypt between 2016 and 2018. The details of the sample locations were presented in Fig 1 and

Standard PCR and sequencing
All samples considered positive by qPCR were subjected to standard PCR and sequencing. Primers targeting 969 bp and 1200 bp region of the 16S rRNA gene, respectively, were used to identify Piroplasma and Borrelia. For the identification of Anaplasmataceae, standard PCR were performed with primers targeting a 520 bp fragment of the 23S rRNA gene. The positive samples with 23S rRNA gene were confirmed with Anaplasma genus-specific primers targeting the 525 bp fragment of the rpoB gene. Rickettsia genus-specific primers targeting the gltA gene were used and the positive samples were confirmed by the ompB gene. Moreover, multispacer typing (MST) for C. burnetii was performed by amplifying of three intergenic spacers (Cox2, Cox5 and Cox18). Identification of Filariidae was performed using 18S rRNA primers targeting 1155 bp. All primer sequences used in standard PCR and sequencing are listed in Table 2. All PCR reactions were performed in an Applied Biosystems 2720 Thermal Cycler model (Thermo Fisher Scientific Courtaboef, France) using AmpliTaq 360 Master Mix (Thermo Fisher Scientific Courtaboef, France) according to the manufacturer's recommendations. Negative and positive controls were included in each reaction. PCR products were visualized by electrophoresis on a 1.5% agarose gel stained with Syper Safe stain (Invitrogen, USA) and analyzed using Lab Image software (BioRad, Marnes-La-Coquette, France).
PCR products were purified using NucleoFast 96 PCR plates (Macherey Nagel, EURL, Hoerdt, France), according to the manufacturer's recommendation. The purified PCR products were sequenced using the Big Dye Terminator Cycle Sequencing Kit (Perkin Elmer Applied Biosystems, Foster City, CA, USA) with an ABI automated sequencer (Applied Biosystems). The sequences obtained were assembled and edited using ChromasPro software

Results
In this study, all samples (557) were screened by qPCR. None of the animals were positive for Bartonella sp., while different animal hosts were positive for piroplasms, Anaplasma sp., Rickettsia sp., Borrelia sp., C. burnettii and Filaria sp ( Table 3). Fifty of 557 (8.9%) animal hosts were positive for piroplasms based on 5.8S rRNA qPCR system. Standard PCR and sequencing based on 18S rRNA gene succeeded in amplifying and identifying two Theileria sp.; T. annulata in cattle (14/88), T. ovis in sheep and buffaloes (5/58 and 2/26, respectively) and two Babesia sp.; Ba. bigemina in cattle (1/88) and Ba. canis in dogs For Anaplasmataceae, 172 out of 557 (30.9%) animal hosts were positive for anaplasmoses by 23S rRNA qPCR system. Based on the 23S rRNA gene, only 87 out of 557 animal hosts were successfully amplified by standard PCR, consequently, sequencing identified only 48 out of 557. The overall prevalence of anaplasmoses in different animals was 8.6%, with 28.4% in cattle (25/88), 6.9% in buffaloes (4/58), 7.7% in sheep (2/26), 6.7% in camels (10/149) and 3.4% in dogs (7/203), while goats were free of Anaplasma DNA. BLAST analysis revealed that cattle, sheep and camel were positive An. marginale, including two different genotypes of An. marginale, the first originated from sixteen cattle, two sheep and one camel with 100% (455/455) similarity to those of An. marginale detected in Rh. bursa collected from cattle in France (GenBank KY498335), and another new genotype was detected in two cattle with 99% (454/455) identity to the same reference dataset (GenBank KY498335). Moreover, one case of cattle was positive for An. centrale with 100% identical to An. centrale strain Israel (GenBank NR076686). From cattle and sheep, a genotype of An. ovis was identified with 100% (454/454) similarity to An. ovis in sheep blood from Niger (GenBank KY644694). We found that dogs and camels were positive for An. platys, including two different genotypes of An. platys, one genotype from six dogs and one camel with 100% (458/458) identity to An. platys in dog blood from France (GenBank KM021425) and another genotype from one dog with 100% (458/458) homology to An. platys in dog blood from France (GenBank KM021414). Finally, from cattle, buffaloes, sheep and camels, a new potential Anaplasma sp. was identified including, four different genotypes of this Anaplasma sp., the first genotype from six camels, the second from two camels, the third from one cattle and one sheep and the last from two cattle and two buffaloes with 98% (450/458), 98% (448/458), 98% (447/458) and 97% (446/458) similarity, respectively, to An. platys in dog blood from France (GenBank KM021414). Sequence analysis of this Anaplasma species revealed that this species has a homology score below 99% (more than 10 nucleotides different) and are closely related to An. platys, that means these sequences could be considered as potential new species of Anaplasma and can be called as An. platys-like. The phylogenetic tree showed that the new potential Anaplasma sp. in two separates and well-supported branches (bootstraps 99 and 96) belong to the cluster of An. platys (Fig 3).
To better characterize different Anaplasma genotypes, rpoB genus-specific PCR primers were applied and 23 good quality sequences were identified. The result revealed that, 12 cattle and one sheep were positive for a genotype of An. marginale with 100% (487/487) homology with An. marginale in Rhipicephalus bursa from France (KY498345), and another genotype of An. marginale from one cattle with 99% (486/487) similarity with the same reference dataset. We also identified that cattle and sheep were positive for An. ovis, one genotype was found in two cattle and another in a sheep with 100% (489/489) and 99% (487/489) identical to those of An. ovis in sheep blood from Niger (GenBank KY644695), respectively. From dogs, we identified a new genotype of An. platys obtained from two dogs with 99% (488/489) homology to An. platys in dog blood from France (GenBank KX155493). Finally, from cattle, buffaloes and sheep, a new potential species of Anaplasma. was identified, its sequences had a homology score of less than 90%, confirming that these sequences are likely to be a new potential species of Anaplasma (like 23S rRNA gene). The only two different genotypes (one from two buffaloes and another from a cattle and a sheep) showed a low identity of 89% (432/486) and 88% (431/ 486), respectively, with An. platys in dog blood from France (GenBank KX155493), while identification of the genotype derived from camels failed. Phylogenetic analysis revealed a new potential Anaplasma sp. (An. platys-like) in a separate and well-supported branch (bootstraps 99) with the same clade belonging to An. platys (Fig 4). Rickettsial infection was detected by qPCR targeting gltA gene in dogs (3/557; 0.54%); the other animal hosts were free of rickettsiosis. To identify Rickettsia sp., standard PCR and sequencing were performed using gltA gene, and it was possible to amplify a 728 bp fragment of this gene from these three positive samples. A BLAST search of the obtained sequences with those in GenBank revealed that two different genotypes, one genotype was 100% (728/728) identical with R. africae previously detected in H. dromedarii from Egypt (GenBank: HQ335126), and the other sequence had 99% (726/728) identity with the same reference. Moreover, ompB gene was used to confirm the detection of R. africae-like infection in dogs. Based on the BLAST search, the sequences obtained from dogs were identified as R. africae (GenBank: MN629894) and showed (757/758) 99% similarity with the reference stain of R. africae detected in a traveler returning from Tanzania (GenBank: KU721071). The phylogenetic tree of these R. africae-like in dogs based on gltA was shown in  in the present study (Bold) related to other Anaplasma sp. and Ehrlichia sp. available in GenBank. The sequence of 23S rRNA were aligned using CLUSTAL W and phylogenetic inferences were constructed in MEGA X using Maximum Likelihood based on Tamura-Nei Model for nucleotide sequences with 500 bootstrap replicates. There was a total of 432 positions in the final dataset. The scale bar represents a 5% nucleotide sequence divergence. https://doi.org/10.1371/journal.pntd.0009767.g003 Screening of Borrelia sp. in all animal hosts we found that 3 cattle and 2 sheep were positive for Borrelia sp. (5/557; 0.9%). Standard PCR and sequencing using 16S rRNA gene identified it as Bo. theileri. Alignment of five obtained sequences of Borrelia sp. from our samples revealed that all sequences were identical to each other. Furthermore, comparison of the obtained sequences with sequences from the GenBank database showed that 1139/1143 (99%) identity with Bo. theileri detected in Rh. geigyi in Mali (GenBank: KF569941). The phylogenetic position of this new Bo. theileri genotype was shown in Fig 6. Two out of 557 (0.36%) blood samples from one sheep and one goat tested positive for C. burnetii DNA by qPCR targeting IS1111. MST genotyping was performed using Cox2, Cox5 and Cox18, with only Cox2 successfully identified and the other spacers failing amplification. in the present study (Bold) related to other Anaplasma sp. and Ehrichia sp. available in GenBank. The sequence of rpoB gene were aligned using CLUSTAL W and phylogenetic inferences were constructed in MEGA X using Maximum Likelihood based on Tamura-Nei Model for nucleotide sequences with 500 bootstrap replicates. There was a total of 534 positions in the final dataset. The scale bar represents a 10% nucleotide sequence divergence. https://doi.org/10.1371/journal.pntd.0009767.g004 A BLAST search for the two sequences obtained showed that (351/351) 100% identity with the reference sequences of C. burnetii recorded in GenBank.
Concerning Filariidae, four out of 557 (0.7%) animal hosts collected from three dogs and one camel tested positive for Filaria sp. DNA. By BLAST analyses, two dogs were found to have D. repens with 100% identity to those of D. repens previously detected in a Japanese woman returned from Europe (GenBank AB973229), and another sequence obtained from one dog showed 99% (1114/1119) similarity to Ac. viteae (GenBank: DQ094171). Moreover, S. digitata with (1107/1111) 99% identity to S. digitata from UK (GenBank: DQ094175) was found in a camel. The phylogenetic analysis of these Filaria sp. was constructed and presented in   Table 3).

Discussion
The sustainable and economic progress of developing countries depends mainly on domestic animal resources, as they provide vital food, draught power and manure for crop production, and generate income [85]. However, animal-associated diseases, especially, VBDs are a global burden [2]. Recently, the spectrum of VBDs affecting animals has expanded and the attention of clinicians and veterinarians is growing. Therefore, the diagnosis of VBDs is crucial to develop the epidemiological mapping of these diseases and this can be achieved through the advances in molecular biology [86].
Concerning piroplasmoses, the overall prevalence among animal hosts was 4.1%, including the highest prevalence among cattle 17%, then sheep 8.6%, buffaloes 7.7% and dogs 0.5%. Based on the 18S rRNA gene, two genotypes of T. annulata was detected in cattle from different provinces (El-Wady El-Geded, Beni-Suef, Qena and Beheira) and one case of Ba. bigemina was detected in cattle from Beni-Suef. In accordance to our results, many studies reported the high prevalence of T. annulata compared to other piroplasms in cattle from different provinces in Egypt [87-89]. In the current study, we observed that the majority of cases (10 out of 15) were detected in cattle from El-Wady El-Geded province that in accordance with Al-Hosary et al. [89], who stated that the prevalence of T. annulata in cattle from El-Wady El-Geded province was 63.6%. This finding might be due to the climate in this province, which is dry and sunny throughout the year, which is conducive to tick activity [89]. Likewise, we identified  Family Anaplasmataceae was known to cause human and animal diseases, is transmitted by ticks and has a worldwide distribution [26,97]. In the current study, the overall prevalence of anaplasmosis was 30.9% (172/557) by qPCR, while we obtained only 48 samples with good quality sequences, possible due to the higher sensitivity of qPCR compared to standard PCR or due to the co-infection with family Anaplasmataceae. The overall infection rate of An. marginale was 3.8% (21/557) in cattle, sheep and camels from different localities (Beni-Suef, Qena, El-Wady El-Geded and Cairo). In Egypt, An. marginale was first mentioned in the national report in 1966, after which the disease was reported in numerous provinces [32][33][34]98]. Several studies reported endemicity of An. marginale in cattle [16,28,[31][32][33][34], buffaloes [30] and camels [29]. However, An. marginale was detected for the first time in sheep. To our knowledge, An. marginale has not yet been described in sheep. For the first time, An. centrale was detected in a bovine from El-Wady El-Geded province, Egypt. Anaplasma centrale is closely related to An. marginale but less pathogenic, so it has been used as a live vaccine to protect against bovine anaplasmosis [99,100]. We also found that sheep and cattle from Beni-Suef province (upper Egypt) were positive for An. ovis with a prevalence rate of 0.7% (4/557). To the best of our knowledge, An. ovis has never been detected in cattle and sheep in Egypt. In parallel, a recent study reported that An. ovis was detected in sheep in Menoufia province (one of Delta provinces) [34], implying that this pathogen is widespread in cattle and sheep throughout Egypt. Anaplasma ovis is the etiological agent of ovine anaplasmosis in small ruminants and causes mild and subclinical infections [23]. In Africa, some studies reported An. ovis in sheep from Tunisia [101], Senegal [25] and Algeria [102,103], and in cattle from Algeria [103]. In addition, we found that dogs from Cairo and a camel from Giza province were positive for two genotypes of An. platys, with an infection rate of 1.4% (8/557). In Egypt, An. platys was never molecularly identified in dogs and camels. Later, Loftis et al, [51] detected An. platys in ticks collected from dogs. Anaplasma platys is the causative agent of canine anaplasmosis, which causes severe thrombocytopenia in dogs [104]. Interestingly, we detected that cattle, buffaloes and sheep from Beni-Suef province and camels from Giza and Cairo provinces were positive for a new potential Anaplasma sp. with a prevalence rate of 2.5% (14/557). This probably new species was genetically related to canine An. platys, which is why it was commonly referred to as An. platys-like. This An. platys-like genotype has never been detected in Egypt, except in a recent study where An. platys-like bacterium was detected only in cattle in Menoufia province [34], implying that this new potential pathogen circulates between different animal hosts (excluding dogs that seem to be susceptible for a type An. platys only) and different provinces in Egypt. Later, An. platys-like was detected in various animal hosts such as cattle in Italy [105], Algeria [106] and Tunisia [107], camels in Tunisia [108,109] and sheep and goats in South Africa [110] and Senegal [25]. Various Anaplasma sp. were identified by the 23S RNA gene and which further confirmed by the rpoB gene.
Rickettsioses are VBDs of humans and animals and are mainly transmitted by ticks [35]. In Africa, the human pathogens R. africae, R. aeschlimannii, R. conorii and R. massiliae have been identified in ticks and animals [39][40][41]. In our study, rickettsial DNA was detected in dogs from Capital Cairo with a prevalence of 1.5% (3/203) in dogs. Phylogenetic analysis showed that our genotypes (R. africae-like) clustered in a separate and well-supported branch (bootstraps 94) with R. africae previously detected in Egypt ( Fig 5) [53]. To the best of our knowledge, R. africae has not been previously detected in dogs anywhere in the world. Thus, this is the first detection of R. africae-like pathogens in dog anywhere in the world. African tick-bite fever, a benign disease with severe complications in elderly populations, and transmitted mainly in the south and West Africa by Amblyomma variegatum [35,111]. Likewise, R. africae was identified in other tick genera as Hyalomma sp. [42,53,54,112] and in Rh. sanguineus (the most common tick parasitizing dogs) [113].
Relapsing fever borrelioses caused by group of the spirochete group Borrelia sp. and is transmitted by soft and hard ticks [57]. In the present study, we identified Bo. theileri in bovine and ovine blood for the first time in Beni Suef province, Egypt, with an overall prevalence of 0.9% (5/557). Alignment of five sequences obtained revealed that there is a new potential genotype of Bo. theileri circulating between cattle and sheep in Beni-Suef province, which is 99% identical to Bo. theileri found in Rh. geigyi in Mali [58]. Borrelia theileri is considered one of the relapsing fever borreliae and the etiological agent of bovine borreliosis in cattle, transmitted by hard ticks, mainly Rhipicephalus sp. [114]. In Egypt, Bo. theileri was reported in Rh. annulata collected from donkeys in the same province [115]. Later, Bo. theileri was also detected in Rh. annulata in Egypt [62]. Recently, some studies have detected Bo. theileri in cattle such as Argentina [116] and Cameroon [117]. Similarly, Bo. theileri has been detected in the blood of sheep in Algeria [102]. It appears that, Bo. theilerii is not exclusively pathogenic to cattle.
Q fever is a tick-borne disease that is a major public health concern [65]. The infection in human manifests as acute or chronic febrile disease often associated with endocarditis and abortion [65]. In Egypt, Q fever was first detected in a high-risk group of cattle farmers [118]. Later, many reports demonstrated the prevalence of the disease in goats, sheep, cattle and camels [67][68][69][70][71][72]119,120]. In this study, the overall prevalence of Q fever in sheep and goats from Sinai province is 0.3% (3% in goats and 1.7% in sheep). This result was in accordance with Abdel-Moein and Hamza [71] who reported an overall prevalence of Q fever of 0.9% and 3.4% in goats. PCR and sequencing amplified only Cox2 with a 100% match with the C. burnetii reference recorded in GenBank, while genotyping and sequencing of the positive samples with other spacers (Cox5 & Cox18) failed. This result can be explained by the fact that the high sensitivity of qPCR can detect low DNA concentrations and the lower prevalence of C. burnetii in blood is lower than feces and urine [121,122].
For filarial infections, we detected four cases of filarial infection with an overall prevalence of 0.7%, 1.5% (3/203) in dogs and 0.7% (1/148) in camels. In dogs from the capital Cairo, we identified two different species of Filariidae as D. repens and Acanthocheilonema sp. Acanthocheilonema viteae is the filarial nematode of rodents, while Ac. reconditum is the etiological agent of filariasis in dogs. Also, there is no sequence of Ac. reconditum for the 18S rRNA gene in GenBank. Therefore, we suspect that the identified species is, however, Ac. reconditum. Therefore, this is the first report of D. repens and Ac. reconditum in dogs in Egypt. Subcutaneous dirofilariasis of domestic dogs is caused by D. repens and is common in Africa, Asia and Europe [123]. It is a mosquito-borne nematode that is a public health problem [124]. Acanthocheilonema reconditum colonizes the peritoneal cavity and adipose tissue and can cause skin lesions with allergy and is transmitted by fleas and biting lice [78,125,126]. In Africa, some studies reported microfilariae of Ac. reconditum in dogs in South Africa [127], Côte d'Ivoire [80]. Moreover, a camel from Giza province was positive for filarial nematodes, and was identified as S. digitata. To our knowledge, S. digitata has not been previously detected in camels. Setaria digitata is the natural filarial nematode of the Bovidae and the adult worm is resident in the peritoneal cavity [128,129]. Accidental transmission of S. digitata to unnatural hosts such as horses, donkeys, sheep and goats causes worrisome pathological problems such as corneal opacity and blindness [74,130,[131][132][133].
Finally, we reported 1.3% (7/557) co-infections in animals, with the highest percentage in cattle 5.7% (5/557). Co-infection in cattle is common and has been reported in many studies [33,34,117,134]. We observed that all cases of co-infections including Anaplasma sp. with another pathogen such as piroplasms, Borrelia or even Rickettsia. Regarding the endemicity of VBDs, we observed the most infected region in Beni-Suef province, where the same genotypes or even new potential pathogens circulated between different animal hosts with a risk of transmission to other adjacent provinces and to humans. Furthermore, we observed that the highest prevalence among animal hosts was anaplasmoses (48/557; 8.6%), followed by piroplasmoses (23/557; 4.1%). Molecular analysis revealed an interesting diversity of these VB pathogens in ruminants and dogs. Therefore, further studies are needed for a better understanding of the epidemiological mapping of pathogen-host-vector in this region or even in the whole Egypt.
In conclusion, the current study is the first large-scale epidemiological observational study that performed molecular screening and characterization of multiple vector-borne pathogens in different animal hosts for better understanding of the endemicity of VBDs in Egypt. We identified for the first time An. centrale, An. ovis, a new An. platys-like and Bo. theileri in cattle, a new An. platys-like in buffaloes, An. marginale, An. ovis, a new An. platys-like and Bo. theileri in sheep, An. platys, a new An. platys-like and S. digitata in camels and R. africae-like, An. platys, D. repens and Ac. reconditum in dogs in Egypt. Therefore, ruminants and dogs in Egypt are reservoirs for multiple neglected, emerging and re-emerging vector-borne pathogens, especially new potential pathogens. Our observational study aimed to describe the repertory of possible vector-borne zoonotic pathogens in Egypt. However, convenient sampling approach did not permit us to evaluate the association of identified pathogens with host characteristics and to describe the geographic distribution of pathogens that limited our study. Further studies are needed to determine the pathogen-host-vector connections and other epidemiological factors of VBDs throughout Egypt, as well as to decipher the zoonotic potential of newly identified genotypes and their animals and public health significance.