Wolbachia strain wAu efficiently blocks arbovirus transmission in Aedes albopictus

The global incidence of arboviral diseases transmitted by Aedes mosquitoes, including dengue, chikungunya, yellow fever, and Zika, has increased dramatically in recent decades. The release of Aedes aegypti carrying the maternally inherited symbiont Wolbachia as an intervention to control arboviruses is being trialled in several countries. However, these efforts are compromised in many endemic regions due to the co-localization of the secondary vector Aedes albopictus, the Asian tiger mosquito. Ae. albopictus has an expanding global distribution following incursions into a number of new territories. To date, only the wMel and wPip strains of Wolbachia have been reported to be transferred into and characterized in this vector. A Wolbachia strain naturally infecting Drosophila simulans, wAu, was selected for transfer into a Malaysian Ae. albopictus line to create a novel triple-strain infection. The newly generated line showed self-compatibility, moderate fitness cost and complete resistance to Zika and dengue infections.


Introduction
The Asian tiger mosquito Ae. albopictus is an increasingly prominent vector of arboviruses. This peridomestic and largely anthropophilic mosquito species is an aggressive daytime biter with a capacity to invade both temperate and tropical areas by adapting to different climates and producing overwintering eggs. It originates in tropical Asia, but is now widely distributed across Europe, Africa, the Americas and the Pacific, and the identified transboundary mechanisms involved in its expansion contribute to a high invasiveness and ever-increasing geographic range [1,2].
Ae. albopictus has been incriminated as a vector of more than 20 arboviruses [3]; it acted as the primary vector of some lineages of chikungunya virus (CHIKV) during outbreaks in La Reunion [4,5] and Italy [6]. It is also a well-characterized secondary vector of dengue virus (DENV) [7], with similar competence to the primary vector Ae. aegypti (although a lower propensity to bite humans), and is responsible for autochthonous DENV transmission in Europe [8,9]. Furthermore, in addition to a capacity to be artificially infected with Zika virus (ZIKV) in a laboratory setting [10], field-collected Ae. albopictus in the Americas were recently found to be ZIKV-positive [11].
Given the enormous impact on public health, and economic impacts imposed by the management of outbreaks in terms of direct and indirect medical costs, there is a need for investment in arbovirus prevention programmes [12,13]. Due to the compromised efficacy and sustainability of traditional vector control approaches-mainly based on the use of insecticidesnovel control strategies are being explored, such as biological control using transmission-blocking symbionts. Among the most promising candidates are Wolbachia, maternally inherited intracellular alpha-proteobacteria commonly found in a wide variety of arthropods; several strains have been shown to provide resistance to different RNA-viruses [14][15][16][17][18], especially when artificially transferred into non-native hosts [19][20][21][22][23]. Many strains of Wolbachia induce a reproductive distortion in the host known as cytoplasmic incompatibility (CI) [24], which occurs when the sperm of Wolbachia-carrying males is modified, resulting in early embryonic death when these sperm fertilize eggs from non-carrier females. In contrast, females carrying the same Wolbachia strain are able to 'rescue' the sperm modification, producing viable offspring.
Ae. albopictus naturally carries two strains of Wolbachia, wAlbA and wAlbB [25], although at a relatively low overall density and mostly confined to the reproductive organs. There is evidence that density and bacterial tissue tropism play a role in Wolbachia virus-blocking ability. In fact, when wAlbB is transferred from Ae. albopictus into the non-native host Ae. aegypti, its density and tissue tropism increase, together with the ability to protect the host against arboviruses [17,21,26].
Several strains of Wolbachia have been characterized in different hosts, revealing considerable diversity in terms of intracellular density, fitness effects, susceptibility to high temperatures, expression of CI and pathogen inhibition [27][28][29][30]. The attributes of each strain within a novel host must be thoroughly characterised to ensure optimal deployment Wolbachia for disease control; there may be a balance between the level of virus blocking and host fitness costs, which will increase the threshold frequency that must be exceeded before it will spread and become established in populations. A stable Ae. albopictus line carrying the wMel strain, transferred from its natural host Drosophila melanogaster, showed reduced vector competence for DENV and CHIKV [31,32]. However, this strain has been shown to be susceptible to density reductions and maternal transmission leakage in Ae. aegypti, when larval stages were exposed to high temperatures [27,33].
A Wolbachia strain from Drosophila simulans, wAu, has been stably transferred into Ae. aegypti, to which it provides unusually high level of inhibition of Semliki Forest Virus (SFV), DENV and ZIKV; additionally, there is evidence that this strain is less susceptible to heat-stress more than wMel [17]. Similarly in its natural host, this strain reaches high densities and protects against a wide range of Drosophila viruses. Although it does not induce CI, spread to relatively high frequency has been reported in field populations of D. simulans in Australia; this may be a consequence of increased host fitness [34]. In light of this, wAu was selected for transfer into a Malaysian Ae. albopictus line to create a novel triple-strain infection and characterized for parameters relevant to its potential field application for disease control.

Mosquito rearing
The Ae. albopictus wild-type strain (JF) collected in the Jalan Fletcher area of Kuala Lumpur, Malaysia, and maintained for >20 generations, was provided by W.A. Nazni, Institute for Medical Research, Kuala Lumpur. A wAu-infected Ae. aegypti line [17] was used as the Wolbachia source. The wMel single-infected Ae. albopictus line was generated as previously described [31]. Mosquito colonies were maintained at standard 27˚C and 70% relative humidity with a 12-hour light/dark cycle. Larvae were fed on tropical fish pellets (Tetramin, Tetra, Melle, Germany) and adults were offered 5% sucrose solution ad libitum. Blood meals were provided using a Hemotek artificial blood-feeding system (Hemotek, UK) using defribrinated sheep blood (TCS Biosciences, UK). Eggs were collected on wet filter paper (Grade 1 filter paper, Whatman plc, GE healthcare, UK) and desiccated for 5-10 days before hatching in de-ionized water containing 1g/L bovine liver powder (MP Biomedicals, Santa Ana, California, USA).

Generation of JF.Neg and JF.wAu (wAlbA-wAlbB-wAu) lines
The Wolbachia-free JF.Neg was generated by treating adults with 1.25 mg/ml tetracycline dissolved in 5% sugar solution [35]. After 4 generations, antibiotic-treated mosquitoes were 100% negative when analysed by qPCR, with no impact on general fitness, egg hatchability, larvae mortality and fecundity.
The generation of a triple-strain (wAlbA-wAlbB-wAu) line in Ae. albopictus involved the transfer of cytoplasm from embryos of the Ae. aegypti line carrying strain wAu, origin Drosophila simulans from Australia [17]; microinjections were performed as previously described [17]. Female survivors (G 0 ) were back-crossed to JF.Neg males, blood-fed and separated individually for oviposition. Strain specific-PCRs for target Wolbachia strains were performed, and only eggs from positive G 0 were hatched. Progeny were also assayed for Wolbachia using PCR to assess germ-line transmission. Positive females were backcrossed with JF.Neg males and their progeny were selected until G 5 , when the line was self-compatible and stable for maintenance.
Wolbachia strain-specific PCR and qPCR in whole mosquitoes and tissues gDNA was extracted from 5-day old whole mosquitoes and tissues (ovaries, guts and salivary glands) using STE buffer (10uM Tris HCL pH 8, 100mm NaCl, 1mm EDTA) and diluted to 100 ng/μl using a NanoDrop spectrophotometer (Thermo Scientific, Waltham, Massachusetts, USA). Strain specific PCR was used for the screening of the newly generated line. Primers sequences are summarized in S1 Table. PCR reactions were set up using 1x Taqmaster mix (Vazyme) according to the manufacturer's protocol. DNA was amplified with an initial denaturation at 94˚C for 3 min, followed by 30 cycles consisting of denaturation at 94˚C for 30 s, annealing at 55˚C for 30 s, extension at 72˚C for 30s, and a final step at 72˚C for 10 min. Samples were then visualized on a 1% agarose gel stained with SYBR safe DNA Gel Stain (ThermoScientific, UK).
Wolbachia density was assessed by qPCR using the relative quantification of the Wolbachia surface protein (wsp) gene against the homothorax gene (HTH) as reference gene. To specifically quantify the wAlbA, wAlbB, and wAu strains, and for the measurement of total Wolbachia density the following primers were used: wAlbA-qAlbAF and qAlbAR; wAlbB -183F and QBrev2; wAu-wAuT3F and wAuT3R; wsp-WSP-F and WSP-R. The following program was used to run the qPCRs: 95˚C for 5 min, 40× cycles of 95˚C for 15 s and 60˚C for 30 s, followed by a melt-curve analysis. A Rotor Gene Q (Qiagen) was used with 2x QuantiNova SYBR.

Adult longevity
A cohort of 25 females and 25 males of JF.wAu, JF.Neg and JF was used for assessing adult survival. Individuals were maintained in 24.5x24.5x24.5cm rearing cages in the insectary, under standardized control conditions. Mosquitoes were blood-fed once a week and eggs were collected two days after the feeding. Mortality was monitored daily until no individuals were alive. Three biological replicates were performed.

Fecundity and egg survival
Fecundity rate and egg survival were assessed using blood fed, fully engorged JF.wAu females. JF and wAu-Ae.aegypti were included as controls. 20 individuals were individualized inside up-turned cups on top of filter paper. After 3 days, the number of eggs laid per female was counted under a stereoscope.
Additionally, the impact of desiccation on egg survival was measured on collected eggs from blood-fed 5-day old females. Sections of filter papers with 300-400 eggs were cut and stored at 27˚C and 70% relative humidity. After 5, 15, 30, 50 days post oviposition, sections were floated in water containing 1g/L bovine liver powder. Hatch rates were assessed by counting hatched eggs and L3-L4 instar larvae.

Maternal inheritance and CI
CI induction between crosses with JF, JF.Neg and JF.wAu was evaluated between 25 males and 25 females in separated cages. Females were offered a blood meal and individualised for oviposition. Eggs were collected, desiccated for 5 days, counted and then, hatched in water containing 1 g/l bovine liver powder. Hatched larvae were counted to estimate egg hatch rates.
Maternal transmission of each of the three Wolbachia strains present (wAlbA, wAlbB and wAu) was evaluated by backcrossing JF.wAu females with JF.Neg males. Females were bloodfed and individualized for oviposition. Eggs, collected on damp filter papers were desiccated for 5 days and added to water for hatching. A selection of progeny was randomly sampled and analysed with strain-specific PCR.

Fluorescent in situ-hybridization
Ovaries were dissected from 5 day old females using sterile forceps and needles in a drop of sterile PBS buffer for Fluorescent In Situ Hybridization (FISH) and then transferred in Carnoy's buffer (chloroform:ethanol:acetic acid, 6:3:1) and fixed at 4˚C overnight. Samples were then rinsed in PBS and incubated in a hybridization buffer containing 50% formamide, 25% 20xSSC, 0.2% (w/v) Dextran Sulphate, 2.5% Herring Sperm DNA, 1% (w/v) tRNA, 0.015% (w/ v) DTT, 1% Denhardt's solution, and 100 ng/ml of each probe. General Wolbachia probes annealing on the wsp gene were used as described before [16].
Samples were left to hybridize overnight in a dark-humid box at 37˚C, washed twice in a solution containing 5% 20xSSC, 0.015% (w/v) DTT, and twice in a solution of 2.5% SSC, 0.015% (w/v) DTT in dH2O, at 55˚C for 20 minutes. Tissues were then placed on a slide containing a drop of VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, California, USA) and were visualized using a Zeiss LSM 880 confocal microscope (Zeiss, Oberkochen, Germany).

Heat-stress response
Eggs from wild-type colonies, JF.wAu and wMel-carrying Ae. albopictus were hatched and separated into experimental groups: larval density (200 larvae per 500 mL of water) and food were consistent between conditions. Heat-challenged larvae were maintained in Panasonic MLR-352-H Plant Growth Chamber incubator (Panasonic, Osaka, Japan). The temperature regime replicated in cabinets was a diurnal cycle of 27-37˚C, while control larvae were reared at constant 27˚C. Adults were all maintained at constant 27˚C. Wolbachia density was quantified by qPCR on whole-bodies of 5-day old females and males, using wsp primers and wAu-specific primers.

Virus challenge
Four cohorts of mosquitoes have been involved in the study for assessing JF.wAu susceptibility to viruses: i) DENV2-blood fed, ii) DENV2-injected, iii) ZIKV-blood fed and iv) ZIKVinjected mosquitoes. 3-5-day old JF.wAu and JF females were blood-fed with human blood, 5mM of phagostimulant ATP and virus suspension using an artificial membrane feeding. Dengue virus was serotype 2 (New Guinea C Strain) at the final concentration in the blood meal of 10 8 FFU/ml, while Zika virus was strain MP1751, obtained from Public Health England culture collections, at a final concentration in the blood-meal of 4x10 7 FFU/ml. Viruses were serially passaged in Ae. albopictus C6/36 cells (three times for DENV2 and once for ZIKV): the infected supernatant was harvested, titered via fluorescent focus assay (FFA) and then frozen until use.
Engorged females were transferred in a climactic chamber at 27˚C and 70% relative humidity for 14 days with access to 5% sucrose-soaked cotton wool. At the same time, a different cohort of females was anaesthetised on ice and injected with 10 9 FFU/ml of DENV-2 in the thorax using a Nanoject II (Drummond Scientific, USA) hand-held microinjector. Similarly, 1.4x10 7 FFU/ml of ZIKV virus was injected in the thorax of females.
Salivary glands of ZIKV blood-fed and heads of DENV blood-fed females were dissected in sterile conditions and transferred in Dulbecco's Modified Eagle Medium (DMEM) medium supplemented with 2% fetal bovine serum (FBS), After being homogenized, the solution was transferred onto a monolayer of Vero cells for FFA. Primary antibody for Zika was a Mouse Monoclonal antibody DIII1B kindly provided by Prof. Arvind Patel (MRC-University of Glasgow-CVR); secondary antibody was the Goat anti-mouse Alexa Fluor 488, A-11001 (Thermo Scientific, Waltham, Massachusetts, USA). Celigo Imaging Cytometer (Nexcelom Bioscience, Lawrence, Massachusetts) was used for imaging plates.
Carcasses of dissected females were sampled in Trizol for quantification of ZIKV infection. RNA was extracted with Trizol according to manufacturer's guidelines and diluted to 100ng/ul with ddH20; cDNA was synthesised All-In-One cDNA Synthesis SuperMix (Biotools, TX, USA). DENV-2 was quantified by qPCR using NS5-F and NS5-R set, while ZIKV 835 and ZIKV 911c primers were used for ZIKV quantification. Values were normalised to the RpS17 as reference by relative expression (Pfaffl method).

Statistical analysis
Graphics were generated using the 'ggplot2' package of R Studio (RStudio Inc., Boston, Massachusetts, USA) of the R software (version 3.6.1). All statistical analyses were run using Prism version 7. Shapiro-Wilk Test was used for assessing normality distribution of data, and parametric and non-parametric tests were selected accordingly. Multiple comparisons were performed using Multiple t-tests and one-way ANOVA using Holm-Sivak's or Bartlett's tests. Survivals were statistically analysed using proportional hazard ratio model (Log-rank-Mantel-Cox test). Analysis of virus-challenged mosquitoes was performed using a Fisher's exact test comparing rates of virus-positive and virus-negative samples.

Strain generation and characterization
Maternal transmission and CI. The JF.wAu line was created by transferring cytoplasm from wAu-carrying Ae. aegypti into wild-type (wAlbA and wAlbB-carrying) Ae. albopictus, obtaining a stable triple-strain infection. From G 1 to G 5 , females were backcrossed with Wolbachia-negative males, blood-fed and individualized; the progeny were pooled only if the mother was positive for all the three strains. From G 5 , colonies were maintained without backcrossing and selecting females: random individuals tested positive in the following generations, indicating that the maternal vertical transmission of the three strains was complete under standard rearing conditions.
Crosses were set up in order to investigate patterns of compatibility and/or CI between the groups ( Table 1). As expected, wAu was not able to induce unidirectional CI when males of JF. wAu were crossed to wild-type females (JF). The JF.wAu line was self-compatible with a similar hatching rate when compared to wild-type. Complete CI was observed between Wolbachia-cured females mated with wild-type or triple-strain males, confirming that the wAlbA / wAlbB strains are still able to induce sterility in the presence of wAu.
As observed during line selection, JF.wAu showed complete maternal transmission of all three Wolbachia strains to progeny: wAlbA, wAlbB and wAu were found in 100% of the 100 individuals analysed among the progeny of individualized females.
Wolbachia density and tissue distribution. Wolbachia density from the whole-bodies of mosquitoes was quantified 5, 10 and 20 days post-eclosion ( Fig 1A). Total Wolbachia in JF. wAu was found to be significantly higher than wild-type after 10 and 20 days post eclosion, similar to what was previously described in wMel-single and triple infection in Ae. albopictus [31,36]. Nevertheless, when compared to wAu in Ae. aegypti, density is significantly lower, lending further support to previous findings that, when transferred into novel hosts, Wolbachia undergo different density modulations [17,23]. The density of the native wAlbA and wAlbB infections appeared to be unaffected by wAu co-infection. (Fig 1B). There is evidence that the strength of viral blockage and the ability to be maternally inherited can also depend on Wolbachia tissue tropism [37]. Total Wolbachia density was quantified on dissected ovaries, salivary glands and midguts (Fig 1C, S1 Fig): generally, the JF.wAu line showed relatively higher densities in somatic tissues, reaching very high density in the ovaries when compared to wild-type (wAlbA and wAlbB) (S1 Fig). Fitness characterization. Additionally, several traits of the fitness of the newly generated line were characterized: adult lifespan, fecundity and fertility of females (Fig 2). Although significantly reduced when compared to wild-type (p<0.001, Log-rank, Mantel-Cox test), JF.wAu females showed a similar life span to the Wolbachia-cured Ae. albopictus (Fig 2A). The survival of JF.wAu males, on the contrary, was not affected by wAu and was not different from the wildtype strains (Fig 2B). Fertility of individualized females was compared between JF.wAu, JF and Ae. aegypti-wAu showing no significant difference on the amount of laid eggs (Fig 2C). In contrast, the co-infection of wAu seemed to have an impact on the hatching rate of desiccated eggs of the JF.wAu line. After 15 days, the number of hatched eggs notably dropped with respect to wild-type eggs (p<0.01, ANOVA, Dunnett's comparison), similarly to Ae. aegypti-wAu, further confirming that high density strains have an impact on egg quiescence (Fig 2D).

Effects of high temperature exposure
Exposure to high temperatures during larval development is known to reduce the density of some strains of Wolbachia in Ae. aegypti, including the wMel strain [17,27]. Consequently, the effects of high temperature exposure were investigated in a previously generated Ae. albopictus line carrying only the wMel strain [31,32], and in the newly generated JF.wAu. Larvae were exposed to a diurnal cycle with fluctuations from 27 to 37˚C in a programmable incubator. The temperature regime reflects realistic peak temperatures in tropical areas, including Malaysia, in water drums and temporary reservoirs acting as natural breeding sites in urban settings.
The general Wolbachia density in different lines was assessed by qPCR in 5-day old females and males. As observed in Ae. aegypti, the wMel strain was particularly sensitive to exposure to high temperatures, showing a decrease in density of several orders of magnitude (Fig 3).
When transferred into Ae. aegypti, wAu was somewhat sensitive to thermal stress, and its density in adults underwent a significant decrease [17]. Similarly, the total Wolbachia density of the JF.wAu lines displayed a drop in heat-treated individuals compared to controls maintained at constant 27˚C, although mean density remained considerably higher than observed for wMel (Fig 3). The density of the native strains in Ae. albopictus wild-type, wAlbA and wAlbB, did not appear to be perturbed by exposure to high temperatures. Consequently, the specific contribution of wAu to the drop in density was further investigated: the quantitative analysis confirmed a significant decrease in wAu in females and males, suggesting a similar response to that observed in Ae. aegypti.

Virus inhibition
DENV-2 and ZIKV were used to evaluate the ability of this line to transmit arboviruses. Mosquitoes were infected through intra-thoracic injection of the virus or through an infected blood-meal. Whole bodies of blood-fed JF.wAu showed significantly reduced DENV-2 loads in whole carcasses (abdomens, heads and thoraxes), compared to JF, confirming the ability of the wAu strain to be an efficient viral inhibitor in Ae. albopictus (Fig 4A). This was further demonstrated when high viral loads (10 9 FFU/ml) were intra-thoracically injected directly into the mosquito haemolymph, bypassing the natural midgut barrier: a significant reduction of DENV-2 genome copies was observed between the triple-strain line compared to wild-type ( Fig 4A). A second independent DENV challenge (Fig 4B) was performed: in this instance, after 14 days, mosquito heads and thoraxes were analysed by FFA in order to assess virus dissemination, while abdomens were analysed separately by qPCR. This replicate provided further validation, confirming the complete inhibition of the virus in both tissues.
The highly protective phenotype was found to be consistent when mosquitoes were challenged with ZIKV. Similar to the DENV challenges, even when very high viral titers were directly injected into the female hemolymph, the wAu strain is able to inhibit the dissemination and the transmission of the virus (Fig 4C). Similarly, no virus was detected by qPCR on carcasses or by FFA on salivary glands of the JF.wAu line after three independent ZIKVinfected blood meals, compared to the wild-type (Fig 4D).

Discussion
Wolbachia-based field interventions to control arboviruses are under investigation in a number of countries for Ae. aegypti, demonstrating promising efficacy for mosquito population suppression and replacement strategies. Nevertheless, dengue control in many endemic regions still remains a challenge due to the sympatric secondary vector Ae. albopictus. Its abundance in many urban and suburban spaces, and its low active dispersal activity makes this vector a suitable candidate for releases in confined urban sites of endemic regions [38,39]. Recently, the use of an artificial triple-Wolbachia infection combined with the sterile insect technique (SIT) for population suppression interventions demonstrated a successful reduction of Ae. albopictus population density in open field trials in China [40]. Additionally, positive results of IIT (Incompatible Insect Technique) for population suppression were also obtained after preliminary trials in Italy of released of Ae. albopictus carrying the strain wPip [41,42]. A) DENV2 genome copies were quantified in whole bodies of 3-5 day old mosquitoes using qPCR on injected and blood-fed females after 14 days. B) Viral genome copies and foci number were quantified after a DENV2-infected blood meal on abdomens and heads. C) ZIKV genome copies (in carcasses) and viral foci number (in dissected salivary glands) of injected and D) blood-fed 3-5 day old mosquitoes. The latter graph summarises three independent biological replicates; different shapes represent data points from each replicate. p<0.001, ��� p<00001. https://doi.org/10.1371/journal.pntd.0007926.g004 As a promising option for population replacement transmission-blocking approaches, a line carrying only the Ae. albopictus wMel strain was previously shown to produce no detectable fitness costs in this host, to induce bidirectional CI and to be refractory to DENV and CHICK in laboratory tests [31,32]. To develop a line with greater invasiveness, a triple-strain infection combining wMel with the native strains, wAlbA and wAlbB, was also subsequently created with the aim of generating unidirectional CI: surprisingly however, the line was found to be selfincompatible and thus, not a practical option to be deployed in the field [36]. Additionally, as previously demonstrated in Ae. aegypti, and now also here in Ae. albopictus, the exposure of wMel-carrying larvae to high temperatures during rearing produces large decreases in overall Wolbachia density. The deployment of wMel in Ae. albopictus for dengue control is therefore likely to be impractical in tropical areas where larval stages experience very high larval site temperatures. The exposure of JF.wAu to cyclical high temperatures during the larval stages resulted in a moderate impact on the overall density of this Wolbachia strain, in this respect offering a more suitable dengue control tool for use in hotter equatorial areas when compared to wMel.
The novel JF.wAu line generated in this study was completely refractory to DENV and ZIKV, and wAu consistently demonstrated complete viral inhibition in both injected and blood-fed mosquitoes. JF.wAu displayed a higher overall Wolbachia density when compared to wild type Ae. albopictus, confirmed in the dissected midgut epithelia, salivary glands and ovaries; this higher overall density is mostly a result of the wAu strain, as was previously observed in Ae. aegypti [17], while the densities of the two native strains are comparable to their wild-type counterparts. The presence of wAu does not cause exclusion or significant density reduction of either of the native strains, ensuring complete maternal transmission of the three strains. In contrast to what was previously observed for the wMelwAlbAwAlbB tripleinfection, the JF.wAu line displayed full self-compatibility, and JF.wAu females were fully compatible when crossed with wild-type males.
The intricate pattern of interactions between different Wolbachia strains and their hosts determines the trade-offs between pathogen blocking, host fitness and invasiveness. High density strains are often associated with some detrimental costs for the host: the example of wMel-Pop, as single and triple strain lines in Ae. albopictus, shows how strong viral blockers can be pathogenic for the host [43,44]. In this case, the JF.wAu line imposes a moderate fitness cost in terms of fecundity and survival rate, when compared to JF, although no difference was observed in the number of eggs laid by individual females when wAu is present.
An important factor to be considered with respect to the potential utilization of wAu for dengue control is of course the lack of CI induced by this strain. Nevertheless, despite this absence, wAu has been shown to be able to spread and remain at high frequency in Drosophila simulans natural populations [34], hypothesised to be a result of providing fitness advantages to its host under some conditions [45,46], particularly its ability to provide protection against pathogenic viruses. However, the potential fitness advantages that could be provided by wAu in Ae. albopictus in the field are unknown, since the prevalence and pathogenicity of circulating endogenous insect-specific viruses is not well understood. The identification of genes responsible for inducing CI located in the WO prophage [47,48] also raises the possibility of integrating CI-carrying phage elements into wAu, in which they are known to be absent [49], to convert it into a CI-inducing strain.
Further investigation into the ecological circumstances in which fitness benefits could be provided by wAu in Aedes or its potential conversion into a CI-inducing strain could offer insights into whether and how this strain could be deployed for population replacement approaches, in light of the very efficient virus inhibition it produces. The wAu Ae. albopictus line also provides a very useful new system for comparative investigation of the mechanisms involved in Wolbachia-mediated viral inhibition.
Supporting information S1 Fig. Fluorescent in situ hybridization. Visualization of distributions of general Wolbachia (green) in ovaries of 5-day old females from the wild-type (A), JF.wAu (B), Ae. aegypti-wAu lines (C) and a no-probe control on wild-type (D). Blue stain is DAPI. (TIF) S1 Table. List of sequences of oligonucleotides and probes. (DOCX)