Comparing the performance of urine and copro-antigen detection in evaluating Opisthorchis viverrini infection in communities with different transmission levels in Northeast Thailand

To combat and eventually eliminate the transmission of the liver fluke Opisthorchis viverrini, an accurate and practical diagnostic test is required. A recently established urine antigen detection test using monoclonal antibody-based enzyme-linked-immunosorbent assay (mAb-ELISA) has shown promise due to its high diagnostic accuracy and the use of urine in place of fecal samples. To further test the utility of this urine assay, we performed a cross sectional study of 1,043 people in 3 opisthorchiasis endemic communities in northeast Thailand by applying urine antigen detection together with copro-antigen detection methods. The quantitative formalin-ethyl acetate concentration technique (FECT) was concurrently performed as a reference method. The prevalence of O. viverrini determined by urine antigen detection correlated well with that by copro-antigen detection and both methods showed 10–15% higher prevalence than FECT. Within the fecal negative cases by FECT, 29% and 43% were positive by urine and copro-antigen detection, respectively. The prevalence and intensity profiles determined by antigen detection and FECT showed similar patterns of increasing trends of infection with age. The concentration of antigen measured in urine showed a positive relationship with the concentration of copro-antigen, both of which were positively correlated with fecal egg counts. The data observed in this study indicate that urine antigen detection had high diagnostic accuracy and was in concordance with copro-antigen detection. Due to the ease and noninvasiveness of sample collection, the urine assay has high potential for clinical diagnosis as well as population screening in the program for the control and elimination of opisthorchiasis.


Introduction
Opisthorchiasis is a neglected tropical disease caused by an infection with a small human liver fluke Opisthorchis viverrini. Based on epidemiological and experimental evidence, O. viverrini as well as a closely related species, Clonorchis sinensis, were classified as group I biological carcinogenic agents [1]. Opisthorchiasis is known to cause important public health problems in mainland Southeast Asia, particularly in Thailand, Lao PDR, Cambodia and Vietnam [2,3]. The current transmission landscape consists of a light and uneven distribution of O. viverrini infection in endemic areas, a feature that has consequences for diagnostic accuracy, as well as the role of O. viverrini as risk factor of cholangiocarcinoma (CCA) [4,5]. For the success of any liver fluke control and elimination program, particularly for mapping and facilitating drug treatment, an improved diagnostic method that is suitable to the current endemic conditions is needed [6,7].
To date, definitive diagnosis of O. viverrini infection is achieved by finding parasite eggs in feces, however, such parasitological diagnosis has many drawbacks including false positivity caused by confusion with the eggs of minute intestinal flukes, or by false negativity in light infections and in biliary duct obstruction where no eggs can be detected in feces. Repeated stool examination is required to increase the reliability of the results [8][9][10], but the cost and requirement of an expert microscopist make this method less practical.
Previously molecular and immunological-based diagnostic methods have been developed and applied for the diagnosis of opisthorchiasis [11][12][13][14][15]. Although these methods have provided a better diagnostic performance compared with the parasitological method, they have several drawbacks regarding their sensitivity and specificity according to the abundance of the target genes, antigens or antibodies, and also the presence of inhibitors in clinical samples [7,16]. An antibody-based approach for the detection of circulating antibody has limitations due to the cross reactive nature of the antigens used [17][18][19][20][21] and a positive result does not always indicate active infection by the parasite [19,22,23].
Unlike antibody detection, an antigen detection assay detects a current and viable parasite infection which better reflects the infection status in opisthorchiasis patients. In this regard, monoclonal antibody-based enzyme linked immunosorbent assays (mAb-ELISA) for detecting parasite antigen in fecal samples (copro-antigen) have been introduced and verified in clinical samples [24,25]. The mAb-ELISA provides several advantages over conventional methods since it has a higher specificity, good reproducibility, and can be prepared in large quantities [26]. In addition to previous studies [24,25], our group has reported an improved protocol for copro-antigen detection with high diagnostic performance [27]. Subsequently, in 2015, we reported a novel antigen detection method using urine samples for the diagnosis of opisthorchiasis [28]. Both urine and copro-antigen detection yielded a high diagnostic performance compared with standard fecal examination, but a comparison between these antigen detection methods in the same sample population has not been reported.
In this study, we aim to assess the field application of this method, comparing the diagnostic performances of urine antigen detection with that of copro-antigen detection. The formalinethyl acetate concentration technique (FECT) was used as a reference method. This study was conducted with the residents of 3 opisthorchiasis endemic communities in Northeast Thailand that showed varying prevalence and intensity of O. viverrini infection. We also investigate the quantitative relationships between the levels of O. viverrini antigens in urine and fecal samples and the fecal egg counts determined by the formalin-ethyl acetate concentration technique.

Ethical statement
The human subject protocol used in this study was approved by the Human Ethics Committee of Khon Kaen University, Thailand (reference number HE561478). Written informed consent was obtained from individual subjects and those with the O. viverrini-positive examination by FECT or antigen detection methods were treated with a single oral dose of praziquantel (40 mg/kg body weight).
The experimental protocols for laboratory animal handling for monoclonal and polyclonal antibody production and also parasite antigen production were approved by the Institutional Animal Ethical Committee, Khon Kaen University (AEKKU-NELAC 26/2558). All animals were anesthetized with isoflurane inhalation before immunization. For euthanization, the animals were anesthetized by isoflurane and sacrificed by drawing of blood from the heart. No animals were demonstrated the severe health problems during in this study. The procedure was performed in strict accordance with the guidelines for the Care and Use of Laboratory Animals of the National Research Council of Thailand.

Study area and study participants
This prospective cross-sectional study began in March 2015 and ended in July 2016. The eligibility criteria of the participants were; (i) participants who were native residents in Ban Wa sub-district in Khon Kaen Province (KK), Tao Ngoi sub-district, Sakon Nakhon province (SK) and Nong Khon Thai sub-district, Chaiyaphum province (CP), Northeast Thailand; (ii) participants who agreed to provide both feces and urine on the same day for index and standard reference tests; (iii) participants who were apparently healthy and had no clinical signs or symptoms. The sample size of this study was calculated based on the average proportion of positive O. viverrini cases (28%) with the derivative set at 1.96 and corresponding to a 95% confident level ± 5%. The calculated sample size was 930 participants with a potential loss of sample submission of 20% so that the number of participants in three localities to be recruited was 1,116.
Of the 1,233 participants originally enrolling, 180 were excluded because they either failed to submit clinical specimens (n = 134) or submitted inadequate specimens (n = 56). A total of 1,043 participants fulfilled the inclusion criterion and provided complete clinical samples (Fig  1). There was no statistical difference in demographics data (age, sex, etc.) between the recruited and the excluded participants.

Clinical samples
Feces (10 g) and samples of the first morning, mid-stream urine (5 ml) were collected from the project participants in standard plastic containers and kept in a chilled box (4˚C-8˚C) during transportation to the laboratory within one day of collection. At the laboratory, each feces sample was separated into 2 parts: (i) aliquoted and processed for parasite examination by the formalin ethyl-acetate concentration technique (FECT), (ii) kept in 1.5 ml tube and stored at -20˚C for copro-antigen detection. The urine samples were centrifuged at 1,500 rpm at 4˚C for 15 minutes and the cleared supernatants were aliquoted into new vials and stored at -20˚C until required for mAb-ELISA.

Faecal examination by the quantitative formalin-ethyl acetate concentration technique (FECT)
The procedure for quantitative FECT was similar to the procedure detailed in the previous report [29]. In brief, 2 grams of fresh stool were fixed in 10% formalin and kept at room temperature until processing. After vigorous shaking, the specimens were centrifuged at 2,500 rpm for 5 minutes and the sediment re-suspended with NSS and strained through two layers of gauze. The tube was then centrifuged at 2,500 rpm for 5 minutes and the supernatant discarded. Seven milliliters of 0.85% saline were added to the sediment and mixed, then 3 ml of ethyl acetate were added to the sample tube, mixed thoroughly, and centrifuged at 2,500 rpm for 5 minutes. After removal of the top three layers, the sediment was re-suspended with 1 ml of 10% formalin. The final suspension was examined blind by two parasitologists and the number of O. viverrini eggs were counted in 2 drops sampled from the total suspension. The number of eggs per gram of feces (EPG) was calculated to determine the intensity of O. viverrini and other parasitic infection.

Antigen detection by monoclonal antibody-based enzyme linked immunosorbent assay (mAb-ELISA)
Sample preparation and pretreatment. The frozen urine and feces samples were thawed at room temperature prior to processing. For fecal sample, phosphate buffered saline (PBS) pH 7.2 was added (1: 3 by volume), mixed thoroughly by vortexing and centrifuged at 2,000 rpm at 4˚C for 30 minutes. The resulting supernatants were transferred into new vials and centrifuged at 10,000 rpm at 4˚C for 5 minutes. The urine samples and also the final supernatants of fecal samples were pretreated with an equal volume of 4% trichloroacetic acid (TCA) to remove interfering components as described previously [27,28,30]. The sample tubes were mixed, vortexed and then incubated for 20 minutes at room temperature. Finally, the mixture was neutralized with an equal volume of 0.244 M carbonate buffer (pH 9.6).

Procedure for antigen detection by mAb-ELISA
The protocols for mAb-ELISA for O. viverrini antigen measurements in urine and fecal samples were similar to those previously described [27,28]. Polystyrene microtiter plates (NUNC, Roskilde, Denmark) were sensitized overnight at 4˚C with 100 μl/well of 5 μg/ml of mAb diluted in 50 mM bicarbonate buffer pH 9.6. After coating, the plates were washed three times with normal saline containing 0.05% Tween 20 (NSST) and uncoated sites were blocked with a solution of 5% dried skimmed milk in 50 mM bicarbonate buffer pH 9.6. After incubation for 1 hour at 37˚C, TCA-pretreated urine and fecal extracted samples were added and the plates (100 μl/well) were incubated at 37˚C for 1 hour. Next, the plates were washed five times with NSST and purified IgG rabbit against crude O. viverrini antigen (10 μg/ml) and 2% dried skimmed milk in PBST was added and incubated at 37˚C for 1 hour. After that, 100 μl/well of biotinylated goat anti-rabbit IgG conjugate (1: 4,000) in 2% dried skimmed milk in PBST were added (Invitrogen, CA, USA) and the plates were incubated at 37˚C for 1 hour. After washing three times, horseradish peroxidase-conjugated streptavidin (GE Healthcare, Buckinghamshire, UK) (dilution 1: 5,000) in PBST was added and the plates were incubated at 37˚C for 1 hour. Plates were washed and the substrate (o-phenylenediamine hydrochloride) solution (Sigma, St. Louis, MO, USA) was added and incubated for 20 minutes in the dark at room temperature. The enzyme reaction was stopped with 100 μl/well of 2M sulfuric acid (H 2 SO 4 ) and the optical densities (OD) were read spectrophotometrically at 492 nm with an ELISA reader (Tecan Sunrise Absorbance Reader, Austria). Two well-trained laboratory staff were responsible for the simultaneous sample analysis of the index tests. During the laboratory procedure, the sample IDs were blinded and the laboratory staff had no knowledge of the sample subjects.

Receiver operation characteristic curve (ROC curve)
Known negative and positive urine samples and also fecal samples for O. viverrini infection determined by FECT were used to construct a Receiver Operation Curve (ROC). The cutoff points for diagnosis by mAb-ELISA were calculated using MedCalc software version 9.6.3 (MedCalc Software, Ostend, Belgium).
The OD values were transformed to antigen concentrations based on the standard curves for urine and feces using spiked O. viverrini-ES antigen extract with varying concentrations starting with 5,000 ng and followed by two-fold serially dilutions to produce a standard calibration curve. The separated cut off value was 19.4 ng/ml for urine and 61.2 ng/ml copro antigen detection methods. These were used to determine negative and positive tests. The sensitivity, specificity, and positive and negative predictive values with 95% confidence intervals were calculated and compared between each sample using standard parasitological methods as a reference. In addition, a composite reference standard method which combined both parasitological, urine or fecal antigen detection methods as a reference method was used to assess the diagnostic performance of each method.

Cross reactivity
In order to assess the specificity of the mAb-ELISA for O. viverrini antigen detection in both urinary and copro-antigen detection method, cross reactivity with positive samples of other parasitic infections determined by FECT was assessed.

Statistical analysis
Data recorded in the case report forms were entered into a computer using the Microsoft Excel program and analyzed using SPSS v.22 (International Business Machines, USA). Helminth species-specific fecal egg counts were transformed into number of eggs per gram of feces (EPG). Kendall's tau-b correlation test was used to determine the correlation between urinary-antigen and copro-antigen concentration and also EPG. Performance of the test in terms of sensitivity, specificity, and predictive values was calculated as described elsewhere [31]. The OD values were transformed to a ratio between the OD of the samples and the OD of reference urine or fecal extracted samples. The reliability of urine and copro-antigen detection methods by mAb-ELISA for the diagnosis of opisthorchiasis was analyzed using odds ratios (OR) with 95% confidence intervals (CI) using logistic regression. A statistically significant level was set as p<0.05. We used the following guidelines to interpret the kappa values; � 0 indicating no agreement, 0-0.2, poor agreement; 0.21-0.4; fair agreement; 0.41-06, moderate agreement; 0.61-0.8, good agreement; and 0.81-1.0, excellent agreement [32].

Prevalence and intensity of O. viverrini infection
As shown in Table 1, based on FECT, the prevalence of O. viverrini infection in the three localities was 29-77% with an overall prevalence of 41%. The highest prevalence was in Nong Khon Thai sub district, Chiyaphum (CP), followed by Tao Ngoi subdistrict, Sakon Nakhon (SK) and the lowest was in Ban Wa sub-district, Khon Kaen (KK). The intensity of infection measured by fecal egg counts (EPG) were similar in the three localities which had mainly either no or light infection (EPG<50) with approximately 13% having an EPG>100. The average intensity of infection by locality was 25-127 EPG and the overall intensity was 54 EPG. Other parasitic infections occurred in <9% of the samples and the prevalence by order were S. stercoralis, MIFs, hookworm and Taenia spp. By urine antigen detection, the prevalence of O. viverrini was 46-77% by locality and the overall prevalence was 54%. The antigen concentration was 27-40 ng/ml of urine with an average of 31 ng/ml in all three localities. In the case of coproantigen detection, the prevalence of O. viverrini was 48-78% with an overall value of 56%. The antigen concentration in feces was 75-90 ng/ml of feces and an average concentration of 81 ng/ml.

Age-prevalence and intensity profiles
As shown in Fig 2, the O. viverrini infection determined by FECT showed the prevalence profile that peaked at 30-40 years in CP and SK, but in KK the prevalence increased slowly and peaked at an age >60 years. The intensity profiles increased steeply from 20-30 years and stabilized thereafter in CP and SK. In KK the intensity increased slowly with age and reached a plateau at age 50 years onwards.
The age-prevalence profiles of O. viverrini by urine and copro-antigen detection (Fig 2B  and 2C) shared a similar pattern in CP and SK where they peaked at age <30 years and plateaued thereafter. In the case of KK, the prevalence increased steadily with age and became a maximum at age <50 years. The age-intensity profiles measured by urine and copro-antigen were similar to those by FECT, being highest at age <30 years and slightly less at age <50 years in CP and SK. The intensity profile for KK increased slowly with age and was highest at age <60 years.

Quantitative relationship between urinary and copro-antigen concentrations of spiked samples
In order to construct the standard curves for the calculation of antigen concentrations in urine and fecal samples, measurement of antigens in spiked excretory-secretory antigen of O. viverrini in urine and feces was performed using mAb-ELISA. The relationships between the concentrations of in urine and fecal samples and OD values obtained from mAb-ELISA were assessed by linear regression models. The best-fit linear regression equations for urinary and copro-antigen detection was y = 0.856x-0.831 and y = 0.653x-0.725, respectively. These equations were used to calculate the concentration of antigen in the clinical samples from the project participants (S1 Fig).

Levels of O. viverrini antigen in urine and feces in relation to intensity of infection
In order to determine the effect of EPG on the level of antigen detected in urine and feces, the participants from all localities were combined and then separated into 4 different intensity groups based on EPG determined by FECT (Table 2). Based on the urine and copro-antigen assays, the positive infection rates were 31% of egg-negative subjects by FECT. In egg-positive groups, the positive infection rates were 83-97% for urine and 83-98% for copro-antigen detections. Overall, urine and copro-antigen detections yielded similar positive rates and both assays yielded 14-15% higher positive rates than FECT.
The concentrations of urinary and copro-antigen showed a significant positive correlation with increasing intensity of O. viverrini (EPG) (Kendall's tau-b, p < 0.001; Fig 3A and 3B).

The relationship between urine and copro-antigen concentrations and their diagnostic performances
Based on 1,043 participants who provided both fecal and urine samples for analysis of O. viverrini antigen concentrations, there was a significant positive correlation between urine and copro-antigen concentrations (Fig 4, R 2 = 0.323, p < 0.001). The best fit linear regression equation was y = 0.635+1.283 where y = copro-antigen concentration (log-transformed value; ng/mL and x = urine antigen concentration (log-transformed value; ng/mL). Table 3 shows the diagnostic performance of urine and copro-antigen detections determined in field-collected samples by using FECT as a gold standard. By locality, urine antigen detection exhibited a sensitivity between 86-90% and a specificity between 56-71%. The overall sensitivity in 3 localities was 89.5% (95% CI = 86.1-92.3) and specificity 71.2% (95%  . The diagnostic performance of copro-antigen detection was comparable to that for urine assay with the overall 90.7% sensitivity and 70% specificity. When using combined methods as a composite standard, the performance of FECT in terms of sensitivities was 54%-85%, with the overall sensitivity of 63.6%. The specificities were 60%-98% between localities, with the overall specificity of 94.8%. Urine antigen detection showed the overall sensitivity 86.2% and was 83%-86% between localities. In the case of coproantigen detection, the overall sensitivity was 85.3% and 85%-89% between localities (Table 4).
For ROC curve analysis, with reference to FECT, the AUC for urine antigen detection was 0.791 while it was 0.831 for copro-antigen detection (Fig 5A). Based on the composite gold standard, the AUCs for the diagnostic assay were 0.824, 0.934 and 0.957 for FECT, urine and copro-antigen, respectively (Fig 5B).
The test for agreement between FECT versus urine antigen detection and FECT versus copro-antigen detection revealed moderate agreement, Kappa values (κ) were 0.547 and 0.570, respectively. The kappa tests showed good agreement (0.770) between urine and copro-antigen detection (Table 5).
Additionally, a logistic regression analysis was performed to predict the risk of human opisthorchiasis (odds ratios) based on increasing arbitrary unit of the antigen in urine and fecal samples by mAb-ELISA. The arbitrary unit was defined as the antigen concentration of 28.050 ng/ml for urine antigen and 75.729 ng/ml that predict the odd of one in having opisthorchiasis. The analysis showed that the odds ratio values increased according to the increasing concentration of both urine and copro-antigens (Table 6).

Cross reactivity of urine and copro-antigen detection methods
To determine the specificity of mAb-ELISA, we applied this method to a separate set of urine and fecal extracts derived from participants with known parasite infection other than O. viverrini by the gold standard FECT.

Discussion
In order to overcome the drawbacks of conventional fecal examination, the urine assay for O. viverrini antigen detection offers not only higher diagnostic accuracy, but also the practical Table 2 Comparing the efficacy of urine and copro-antigen assays for diagnosis of opisthorchiasis advantage of utilizing urine instead of feces for the diagnosis of opisthorchiasis [28]. The urine antigen detection method is based on the hypothesis that excretory-secretory antigens released by living parasites can enter the blood circulation and be excreted via the kidneys into the urine. Herein, we applied this method in a community-based epidemiological study of opisthorchiasis, together with copro-antigen detection, using a mAb-ELISA protocol. The results indicate that urine as well as copro-antigen detection have comparable diagnostic accuracy and both methods have a superior diagnostic performance to that of fecal examination by FECT. The estimated concentration of antigen in the urine as well as that in feces significantly positively correlated with the intensity of infection measured by fecal egg count (egg/gram feces). Moreover, the antigen concentration in urine positively correlated with the levels antigen in the feces and the agreement test between methods showed good concordance (kappa Comparing the efficacy of urine and copro-antigen assays for diagnosis of opisthorchiasis value; 0.770). The average antigen concentrations in both urine and feces paralleled EPG. The antigen concentration in urine was two-to three-folds less than those in feces. On average of the antigen concentration in feces was 2.6 times that of urine. Because of the qualitative and quantitative concordance between urine and copro-antigen detection, as well as that from FECT, the ease of sample collection and specimen handling strongly indicate that urine detection is desirable for the diagnosis and screening of opisthorchiasis. There was a higher positive detection obtained by urine (46-77%) and copro-antigen detection (48-78%) compared to FECT (29-77%). The discrepancies of antigen detection versus fecal examination by FECT were seen at low to, moderate intensities of opisthorchiasis, i.e. in SK a geometric mean of 25.3 EPG, in KK a geometric mean of 59.7 EPG. By contrast, the positive rates detected by both urine (77%) and copro-antigen detections (78%) were similar to FECT (77%) in the locality with high transmission intensity (geometric mean 127.1 EPG) in CP. These findings further support the usefulness of the urine antigen detection assay for screening for opisthorchiasis with low intensities of infection. This may help in avoiding the under diagnosis of the majority of individuals who have a low worm burden. However, whether our antigen detection assays can demonstrate a single worm infection, as reported in schistosomiasis [33], is not known, but this is a critical threshold to assess the impact of parasite control and eventually elimination, and hence requires further investigation. Moreover, the occurrence of varying transmission levels observed in the three study communities reflects once again the changing landscape of infection associated with multiple factors such as mass drug administration (MDA) and public health educational efforts [2,34]. Therefore, a detection method which is analytically sensitive yet also rapid and easy to apply is required to monitor the current endemic situation of opisthorchiasis [7].

Copro-antigen n (%)
For cases of O. viverrini egg negativity as determined by FECT (n = 620), 191 (30.8%) and 195 (31.4%) positive cases were discovered by urine and copro-antigen detection, respectively. The finding of antigen positivity in this scenario indicates the vital role of worms rather than Table 3. Diagnostic performance of antigen detection by urine and copro-antigen detection in comparison with the gold standard FECT (n = 1043). eggs as a source of antigen in the urine and feces in opisthorchiasis. A similar situation was previously observed in an autopsy study which revealed that fecal eggs were not discovered in the majority of infected individuals with less than 20 worms [35]. Further study is required to monitor the changes in antigen profile after treatment to justify the need for chemotherapy of the antigen positive but egg-negative individuals.
As opposed to egg negative cases, in cases of positive for O. viverrini eggs (1-100 EPG), not all individuals were found positive by the antigen detection methods. Within the egg positive group (n = 423), 11.3% were negative in urine and slightly less (8.5%) were copro-antigen negative. The explanation for the under diagnosis by antigen detections is currently not known but may depend on several possibilities. First, as the antigens appearing in the urine and feces are excretory-secretory products originating from living adult worms and may not directly relate to fecal egg count. Second, the presence of urine antigen may rely on the intermittent production of secretory products from the adult worms and also the passage through kidney glomerular filtration as proteinuria [36] and motility of gastrointestinal system in the case of copro-antigen. Thus, liver fluke-induced pathology in the biliary system and abnormal glomerular filtration in the kidneys may contribute in fluctuations of urine antigen. Third, the physical properties of urine and feces such as water content and fecal mass may influence the antigen concentration and eventually detectable antigen levels. Dilution effects of urine were Comparing the efficacy of urine and copro-antigen assays for diagnosis of opisthorchiasis  Comparing the efficacy of urine and copro-antigen assays for diagnosis of opisthorchiasis addressed using the concentration procedure to find the accurate antigen detection levels in urine in schistosomiasis [33]. Lastly, more antigen positive cases can be found by multiple or repeated examination of the urine and feces over consecutive days, which yield a higher positive diagnostic rate as shown in the case of schistosomiasis [37,38]. Kidney involvement in opisthorchiasis, in terms of proteinuria and deterioration of renal function associated with immune complex, has been shown previously [39,40]. Since the antigen detection approach in opisthorchiasis is at a rudimentary phase, further work to prove and disprove the above hypotheses is required to augment our understanding of the presence as well as the pathway of urinary antigens, and hence the improvement of diagnostic accuracy. The urine antigen detections had a 0.79 AUC and 89% sensitivity while the copro-antigen detection had a comparable accuracy (0.83 AUC and 90% sensitivity) with respect to the conventional FECT gold standard. A similar trend but with slightly lower diagnostic values was observed when the composite diagnosis was used as a reference standard in which FECT has the lowest sensitivity (63%) but high specificity. The observed diagnostic values in the current study were comparable to our recent study for urine [28] and copro-antigen [27] but higher than that in previous reports for copro-antigen detections [24,25]. This observation is probably due to different mAb-ELISA protocols, particularly with monoclonal antibody being used. Additionally, the diagnostic accuracy of opisthorchiasis may approach a new gold standard when antigen detection is combined with fecal examination, agreeing with an approach used in cases of clonorchiasis [41].
Although O. viverrini-specific mAb was used for antigen detection in ELISA, cross relativities with other parasite antigens in urine were observed in individuals infected with S. stercoralis, hookworms and T. trichiura. In the case of copro-antigen detection, cross reactions occurred with S. stercoralis, MIF and hookworms. These findings are similar to our previous reports [27,28], and may be explained by the fact that these patients probably harbored a low intensity of O. viverrini infection which could not be detected by FECT. Since multiple parasite infections are common in the study areas in Thailand, further cross reaction studies on the participants from non-O. viverrini endemic areas are needed.
In addition to cross reactivity with other parasitic infections, there are drawbacks for both the urine and copro-antigen detection methods that required attention. The main drawback is that the whole process of mAb-ELISA takes approximately 5-6 hours to complete and these assays needed experienced operators and special equipment. Furthermore, samples should be kept cold (4˚C or on ice) during transportation and require centrifugation and pre-treatment with TCA prior to the analyses. Therefore, a "rapid point of care and ease-of-use" platform Table 6. Prediction of human opisthorchiasis by increasing urine and copro-antigen concentration.

Unit increasing in urine antigen
Odds ratio 95% Confidence Interval Comparing the efficacy of urine and copro-antigen assays for diagnosis of opisthorchiasis such as a lateral flow immunochromatgraphic strip or dipstick, which can be operated at point of care setting, should be developed.

Conclusion
In this study, we show that O. viverrini antigen detection in urine and fecal samples has a higher diagnostic accuracy than conventional fecal examination method, FECT. Both antigen Comparing the efficacy of urine and copro-antigen assays for diagnosis of opisthorchiasis detection methods yielded comparable diagnostic accuracy and were significantly positively correlated. With high diagnostic accuracy and the ease of sample collection, the urine antigen detection method is a powerful approach for the diagnosis and population screening of opisthorchiasis in a clinical and also field setting. Pending additional study, the urine antigen detection method may serve not only the purpose diagnosis and screening, but also as a tool to follow-up opisthorchiasis patients to determine effective drug treatment in the control and elimination program.