Matching the genetics of released and local Aedes aegypti populations is critical to assure Wolbachia invasion

Background Traditional vector control approaches such as source reduction and insecticide spraying have limited effect on reducing Aedes aegypti population. The endosymbiont Wolbachia is pointed as a promising tool to mitigate arbovirus transmission and has been deployed worldwide. Models predict a rapid increase on the frequency of Wolbachia-positive Ae. aegypti mosquitoes in local settings, supported by cytoplasmic incompatibility (CI) and high maternal transmission rate associated with the wMelBr strain. Methodology/principle findings Wolbachia wMelBr strain was released for 20 consecutive weeks after receiving >87% approval of householders of the isolated community of Tubiacanga, Rio de Janeiro. wMelBr frequency plateued~40% during weeks 7–19, peaked 65% but dropped as releases stopped. A high (97.56%) maternal transmission was observed. Doubling releases and deploying mosquitoes with large wing length and low laboratory mortality produced no detectable effects on invasion trend. By investigating the lab colony maintenance procedures backwardly, pyrethroid resistant genotypes in wMelBr decreased from 68% to 3.5% after 17 generations. Therefore, we initially released susceptible mosquitoes in a local population highly resistant to pyrethroids which, associated with the over use of insecticides by householders, ended jeopardizing Wolbachia invasion. A new strain (wMelRio) was produced after backcrossing wMelBr females with males from field to introduce mostly pyrethroid resistance alleles. The new strain increased mosquito survival but produced relevant negative effects on Ae. aegypti fecundity traits, reducing egg clutche size and egg hatch. Despite the cost on fitness, wMelRio successful established where wMelBr failed, revealing that matching the local population genetics, especially insecticide resistance background, is critical to achieve invasion. Conclusions/significance Local householders support was constantly high, reaching 90% backing on the second release (wMelRio strain). Notwithstanding the drought summer, the harsh temperature recorded (daily average above 30°C) did not seem to affect the expression of maternal transmission of wMel on a Brazilian background. Wolbachia deployment should match the insecticide resistance profile of the wild population to achieve invasion. Considering pyrethroid-resistance is a widely distributed phenotype in natural Ae. aegypti populations, future Wolbachia deployments must pay special attention in maintaining insecticide resistance in lab colonies for releases.


Methodology/principle findings
Wolbachia wMelBr strain was released for 20 consecutive weeks after receiving >87% approval of householders of the isolated community of Tubiacanga, Rio de Janeiro. wMelBr frequency plateued~40% during weeks 7-19, peaked 65% but dropped as releases stopped. A high (97.56%) maternal transmission was observed. Doubling releases and deploying mosquitoes with large wing length and low laboratory mortality produced no detectable effects on invasion trend. By investigating the lab colony maintenance procedures backwardly, pyrethroid resistant genotypes in wMelBr decreased from 68% to 3.5% after 17 generations. Therefore, we initially released susceptible mosquitoes in a local population highly resistant to pyrethroids which, associated with the over use of insecticides by householders, ended jeopardizing Wolbachia invasion. A new strain (wMelRio) was a1111111111 a1111111111 a1111111111 a1111111111 a1111111111

Introduction
Mosquito-borne diseases heavily impact human health in tropical areas, where some of them co-circulates in several countries. Dengue virus (DENV) is a flavivirus distributed mainly in tropical and subtropical areas, causing around 400 million new infections each year. Brazil has the highest number of DENV cases, with more than one million cases per year for the last three years [1]. Chikungunya virus (CHIKV) was added to the arboviruses of global health concern in the early 2000's, when a new epidemic strain emerged from an enzoonotic lineage. In 2014, the first autochthonous CHIKV cases appeared in Brazil, linked to both the Asian and the East Central South Africa (ECSA) genotypes of the virus, with the last spreading throughout the country [2]. Most recently, the world witnessed the spread of Zika Virus (ZIKV) across the Western Hemisphere within the span of one year. In 2014, ZIKV was introduced in Brazil from the Pacific Islands, and one year later a spike in microcephaly cases among newborn babies prompted the World Health Organization (WHO) to declare a Public Health Emergency of International Concern [3]. Since late 2016, one of the most severe sylvatic yellow fever outbreak has been reported on the gates of the largest metropolitan Brazilian areas highly infested with Aedes aegypti, augmenting the risk of urban outbreaks of YF [4].
All aforementioned arboviruses are or may be transmitted by the mosquito Ae. aegypti, making the control of mosquito populations the best action to mitigate disease transmission. Existing vector control programs in Brazil rely heavily on the use of insecticides and reduction of larval breeding sources to limit vector abundance. However, due to widespread insecticide resistance in wild Ae. aegypti populations and the logistical constraints in extending source reduction over large cities, the effectiveness of traditional control methods in this context is limited [5][6][7]. Therefore, the development of new strategies to supplement traditional vector control methods is of utmost importance to manage mosquito-borne diseases [8].
One of the innovative approaches consists of deploying the endosymbiotic bacterium Wolbachia pipientis to block arbovirus transmission in endemic urban settlements [9][10][11][12][13]. Wolbachia are maternally transmitted bacteria present in around 60% of all arthropod species. Although they do not naturally infect Ae. aegypti, a few strains were transinfected into Ae. aegypti from other insects and their pathogen-blocking properties were discovered [9][10][11][12][13][14][15]. Wolbachia spread through arthropod populations based on its maternal mode of transmission and reproductive manipulations to the host, known as cytoplasmic incompatibility. Using these properties, Wolbachia-infected Ae. aegypti can be released in disease-endemic areas to replace a local mosquito population of high vector competence with others with diminished potential for transmission.
Successful releases of Wolbachia strain wMel have been carried out in central Vietnam and Northern Queensland [13]. Given these promising pilot releases and the limited effect of insecticide-based control campaigns [7], the Brazilian Ministry of Health and the World Mosquito Program (formerly Eliminate Dengue) pursued rolling out wMel strain in the community of Tubiacanga, Rio de Janeiro. After releasing a total of 180,000 Ae. aegypti carrying wMelBr (Brazilian strain) during 20 consecutive weeks, we observed that Wolbachia was unable to spread into the Ae. aegypti population. After exhaustive investigation, we herein report the factors that jeopardized the Wolbachia invasion, the steps required to produce a fit mosquito strain, and the results of a subsequent release on the same site.

Study sites
Mosquitoes infected with the Wolbachia strain wMel were released in the isolated community of Tubiacanga (22˚47'06"S; 43˚13'32"W) in Rio de Janeiro. This is a lower-middle-class community located in a lowland coastal area on the shores of Guanabara Bay comprised of around 3,000 residents in 690 houses [16,17]. During releases, weekly climatic data (mean, minimum and maximum temperature, accumulated rainfall) was collected at a meteorological station located 6 km from Tubiacanga (S1 Fig).

Ethical considerations
Wolbachia releases were authorized by the National Research Ethics Committee (CONEP, CAAE 02524513.0.1001.0008), and further regulatory approval was obtained by three Government agencies: IBAMA (Ministry of Environment), Anvisa (Ministry of Health) and MAPA (Ministry of Agriculture). According to ethical and regulatory approval, door-to-door activity and formal signed consent for the releases was required from at least 70% of the sampled households (around 30% of households were sampled). Adults over 21 years old answered the questionnaires. One strong request of CONEP was that the World Mosquito Program team not change any practices performed by the community or the municipality vector control.
The maintenance of Ae. aegypti colonies in the lab were achieved by blood feeding mosquitoes with human blood obtained from anonymous donors from the blood bank of the Rio de Janeiro State University, whose blood bags would be discarded due to small volume. We have no information on donors, including sex, age and clinical condition, but the blood bank discard those bags positive for Hepatitis B, Hepatitis C, Chagas disease, syphilis, HIV and HTLV. Before offering the blood for mosquitoes, it was screened for DENV using the Dengue NS1 Ag STRIP (Bio-Rad). The use of human blood was approved by the Fiocruz Ethical Committee (CAAE 53419815.9.0000.5248).

Community engagement (CE) and communication (Comms) strategy for Wolbachia release
Before any mosquito releases, community activities were carried out with the purpose of presenting the project, listening to the population, explaining the implications for the community as well as for individuals. CE began with the identification of local stakeholders, in a process of engagement with the community as the initial stage of an action-participant research [18,19].
In order for community bonds to be strengthened and a process of governance and scientific transparency to be established, a Community Reference Group (CRG) was created. The CRG had seven local volunteered representatives (two from the community association, one from local school, one health agent, one merchant and two residents). This stage describes the process of defining the problem and planning of actions [20]. The CRG was built as a space for dialogue between the parties, with shared advances and concerns regarding public health issues, especially those related to vector-borne diseases, and the safety related to Wolbachia releases. The meetings happened monthly and were held, as a rule, at the local residents' association. During the engagement and communication process, lectures were given in schools, training in the local health unit, participation in meetings of local social groups, and door-todoor actions to explain the project. A survey, conducted by an external team, evaluated the consent of local residents supporting releases.
With the need for a second round of mosquito release (wMelRio), new Community Engagement and Communication strategies were developed. Firstly, we focused on our closest partners, the residents' association and the CRG. We explained the reasons for Wolbachia not invading, listened to community expectations and discussed the timeline for the second release. The door-to-door activities were intensified, meetings with householders were increased and we also strengthened the collaboration with the local primary school. The Communications team produced flyers to support CE activities, placed banners in public areas, scheduled doorto-door visits whenever required and provided positive mass media on TV, radio and internet. After these actions, another survey was carried out.

Backcrossing of wMelBr-infected Ae. Aegypti
wMel-infected Ae. aegypti were imported from Australia (IBAMA license 11BR005873/DF) to produce a Wolbachia-infected line with a Brazilian genetic background, named wMelBr (details on [17]). The backcrossed laboratory colony was maintained for 17 generations before the obtaining regulatory approvals for mosquito releases. Subsequently, every five generations we added 10% of wild males from a Rio de Janeiro population mix to refresh the genetic pool of the colony.

wMelBr colony quality assurance
We randomly selected 100 3 rd -4 th instar larvae per generation to estimate the frequency of Wolbachia in the colony, with a threshold of at least 98% positivity to support releases. Larvae were reared with 0.45 g of Tetramin Flakes (Tetra GmbH, Herrenteich, Germany) every two days in 3 L plastic trays at 28˚C. Each week ten of the release cups (50 mosquitoes per cup) were brought back to the lab to assess the fitness of mosquitoes being released. We assessed male and female wing size, adult mortality, and sex ratio. Wing length was defined as the distance from the axillary incision to the apical margin excluding the fringe [21]. Mortality and sex ratio were checked visually as release cups returned to the insectary, when mosquitoes were one week old.

wMelBr and wMelRio releases
The first release started four months after regulatory approval, i.e., 17 generations had elapsed with limited outbreeding (adding 10% wild males every five generations). In the first round of releases at Tubiacanga, 50 mosquitoes 4-6 days old were released in the early morning (5:00 AM) in front of one in every four houses. The deployment of wMelBr began in September 2014 and lasted 20 consecutive weeks, until January 2015. The total number of mosquitoes released per week increased over time, starting with 7,500 on week 1 and ending with 15,000 on week 20. Therefore, we released between 10.86 to 21.74 mosquitoes per house per week. Later, on August 2015, we started deploying wMelRio in Tubiacanga. A similar release protocol was adopted, with 10,000 mosquitoes released every week through 24 consecutive weeks.

Wolbachia detection
Adult mosquitoes were individually screened for the presence of Wolbachia infection using a multiplex RT-qPCR that included the WD0513 gene and a ribosomal gene of Ae. aegypti [22]. The amplification was carried out on a ViiA-7 Machine (Thermo Scientific) using Taqman Universal PCR Master Mix (Thermo Scientific) following manufacturer's instructions. Wolbachia relative quantification was performed according with Caragata et al. [23].

Mitochondrial DNA
To test for imperfect maternal transmission of Wolbachia in released mosquitoes, we conducted the analysis of mitochondrial DNA variation on field-caught females. We screened for a diagnostic SNP within the mitochondrial COXI gene in mosquitoes that were negative for Wolbachia. This SNP is located at the 531bp location in the COI amplicon (A in release strain mosquitoes, and G in wild type mosquitoes). As Wolbachia and mitochondria are coinherited we can infer that individuals that do not contain Wolbachia but carry the diagnostic SNP have arisen from imperfect transmission of Wolbachia [24]. Mosquitoes were trapped 1, 3, 12, 13, 14, 16, 17, 20 and 26 weeks after the release of wMelBr mosquitoes had started. Those identified as Ae. aegypti were tested for the Wolbachia infection status by multiplex qPCR using Taqman probes except the primer set IS5 were not used in this assay. Presence of the diagnostic SNP in the COXI gene was tested in 456 mosquitoes using the Sanger sequencing of a small fragment (970bp) of the COXI mitochondrial gene [24]. Sequence identity was validated using BLASTn at NCBI, and the alignment was performed using SeqMan Pro (Lasergene, Madison, USA).

wMelRio backcrossing
When the need for releasing mosquitoes resistant to pyrethroids into field populations became apparent, we started a second backcrossing using 500 females from wMelBr colony and 500 males from Urca, an urban district from Rio with high pyrethroid resistance. To infer pyrethroids resistance throughout backcrossing, we analyzed kdr mutations frequencies. These mutations have been described as an important insecticide resistance mechanism that contributes with high levels of pyrethroid resistance in Rio de Janeiro Ae. aegypti populations [25][26][27]. Moreover, kdr scoring is a rapid and simple tool when compared with bioassays. During backcrossing, we had a total of six populations, each one representing one step of backcrossing to produce wMelRio (wMelBr, Field (Urca), wMelF1CP, wMelB1, wMelB2, and then wMelRio). We added a seventh population, wMelBrTet, which was treated with tetracycline by three successive generations to remove Wolbachia and maintain the mosquito genetic background [17] (S2 Fig). Doing so, we expected that the frequency of kdr alleles increased over time, what was tested by Wolbachia and kdr screening of 100 mosquitoes randomly sampled for each generation.

Insecticide resistance bioassays in field and released mosquitoes
To determine the insecticide resistance profile, we performed bioassays on wMelBr, wMelRio, and field mosquitoes from Tubiacanga, Jurujuba and Urca. The susceptible Rockefeller strain was used as a control. Dose-response bioassays were performed with third instar larvae to determine their susceptibility to the organophosphate temephos (Pestanal, Sigma-Aldrich) and the chitin synthesis inhibitor diflubenzuron, and with 3-5-day-old adult females to the pyrethroid deltamethrin and the organophosphate malathion using WHO test tubes [29,30]. Lethal concentrations were calculated with Probit analysis [software Polo-PC, LeOra Software, Berkeley, CA] [31]. Resistance Ratios (RR 50 and RR 90 ) were obtained by dividing the lethal concentrations (LC) or knockdown time (KdT) values of the test populations by those of the Rockefeller reference strain [29,30].

Estimating fitness costs of pyrethroid resistance in the laboratory colony
The frequencies of the kdr allele were obtained from a sample of 100 mosquitoes of F1 and F18 generations from the wMelBr colony. Wild males were added every five generations to refresh the colony, and consequently resistance alleles were introduced. We estimated the fitness cost due to insecticide resistance by applying a Monte-Carlo procedure over a two-allele model. A fitness cost applies for the genotype of homozygous resistant alleles (R1R1 and R2R2, similarly), whereas heterozygous individuals have a smaller cost given by a multiplicative factor of 0.9 as used by Hoffmann and Turelli [32] in the case of resistance nearly recessive. Successive wMelBr crossings were simulated for 17 generations assuming these fitness costs. In generations F10 and F15 we also simulated crossings with an added male population of resistant homozygous individuals by a ratio of 1 male: 10 females. After running 100,000 simulations, we obtained a subset of results for which the predicted frequencies in F18 were equal to the observed frequency for the F18 generation ±1%.

Fitness assays
Mosquito life-history traits were measured in the backcrossed strain to test whether the addition of alleles to promote pyrethroid resistance would affect the fitness of Wolbachia-infected mosquitoes (S2 Fig). Development time. Eggs of the seven tested populations were hatched, and three groups of 100 first instar larvae per population were transferred to plastic trays (33 X 24 X 8 cm) and monitored daily for 14 days to assess the number of pupae per day. Survival analysis was performed to determine the time to pupation in the populations.
Adult survival. Sixty females per population were selected and individually isolated in labeled cylindrical plastic vials (6.5 cm height, 2.5 cm diameter). Adults were maintained under insectary conditions (80 ± 5% humidity and 25 ± 3˚C) and fed ad libitum with 10% sucrose solution for up to 36h before the blood feeding [33]. Survival was checked daily and dead mosquitoes were stored in a -80˚C freezer for subsequent Wolbachia and kdr detection. Ae. aegypti longevity was normally distributed (Shapiro-Wilk W = 0.9942, P = 0.113). The effect of Wolbachia infection and density, frequency of kdr mutation and its interaction on mean Ae. aegypti longevity was analyzed by a three-way ANOVA. A Log-rank test followed by Bonferroni correction compared the survival distribution of Ae. aegypti females. We estimated by Cox proportional risk the hazard ratio of Wolbachia density and kdr frequency on Ae. aegypti survival.
Oviposition success, female fecundity and egg hatch. Females were blood fed with human blood once a week and eggs were counted 3-4 days later. Fecundity was analyzed by considering the first five clutches of eggs laid, since only a small number of females laid eggs beyond the fifth clutch, precluding adequate numbers for analysis. We analyzed the likelihood that a mosquito laid at least one egg (at a given clutch) with a logistic analysis that included Wolbachia presence and density, frequency of kdr mutation and clutch-number (i.e., age), backwards-eliminating the non-significant interactions. Second, we analyzed the number of eggs of the successful mosquitoes with a MANOVA repeated measures analysis. We included clutch-number as the repeat and estimated the effects of Wolbachia infection, Wolbachia density and frequency of kdr mutation.
Eggs laid on filter papers were kept for four days at room temperature to allow for embryo development. Eggs were counted and the batch was hatched in plastic cups with Tetramin as a food resource. Larvae were counted and divided by the number of eggs to assess mosquito fertility. Egg hatch rate was monitored during the four first oviposition cycles.
Maternal transmission. All adult females and a sub-sample of up to 20 larvae per female were screened for Wolbachia on weeks 1 and 4. To assess whether there was any failure of maternal transmission and, if present, to test if it was correlated with the introduction of kdr during backcrossing.
Statistical analysis was conducted on R version 3.3.2 and JMP 13.

Genetic similarity among release and field mosquitoes
To assess the level of genetic similarity between released material and wild mosquitoes, we used double-digest RAD-sequencing, as previously described [34]. We analyzed 25 mosquitoes from each sample: the released colonies (wMelBr and wMelRio), field-caught individuals from Tubiacanga and Gordonvale (Queensland, Australia, as a proxy for the original wMel transfected colony). The 100 bp paired-end reads from the Illumina HiSeq platform were deposited to NCBI (SRA PRJNA273913, PRJNA330553). The raw sequences were processed through a previously published pipeline [34], retaining high-quality reads (Phred >20), trimmed to 90 bp, and uniquely aligned to the Ae. aegypti genome sequence (AaegL1) using Bowtie v0.12.7 [35]. SNPs and genotypes were called in the program Stacks v1.36 [36], and further filtering of markers was done in the program VCFTools v0.1.12b [37]. Genome-wide similarity among samples was analyzed using the Discriminant Analysis of Principal components [38] in the R package adegenet [39]. The index of genetic structuring (F ST ) was calculated in VCFTools using the Weir and Cockerham's AMOVA-based method [40].

Community approval
An action-participant research requires a qualitative-quantitative analysis, thus, throughout the process of engagement and communication, some indicators sustained the success of the adopted actions. The CRG summing the willingness of local institutions to develop projectrelated activities, indicated that the acceptance of the community, since the proposed activities were not mandatory and there was no resistance from the actors involved. Moreover, it has identified a growing involvement of the community, willing to act cooperatively and actively in the territory. In this process, a sense of belonging was identified. In informal communications, we identified that the locals were proud to be the first Latin America neighborhood to participate in the project and receive a Wolbachia release, bringing a positive agenda for that community. A house-to-house survey was conducted in 268 (38.8%) houses in Tubiacanga in June/2014. A total of 235 (87.7%) householders declared support to mosquito releases (wMelBr strain), 20 (7.4%) opposed releases and 13 (4.85%) did not answer this specific question. A new survey was required after the second trial was confirmed, and the approval was over 90% in Tubiacanga, demonstrating long-term and strong community support for Wolbachia deployment.

wMelBr deployment in Tubiacanga
The Wolbachia frequency in field-caught mosquitoes increased smoothly over weeks 1-6 of wMelBr deployment. Surprisingly, the frequency plateaued between weeks 7-19 despite releases involving a total of 15,000 mosquitoes per week. On the last week of releases (week 20), we observed a peak of Wolbachia frequency of 65% (Fig 1). However, wMelBr frequency dropped dramatically as soon as the releases stopped. For instance, just five weeks after the releases had ended, the Wolbachia frequency had fallen to around 20% (Fig 1). Interestingly, wMelBr did not disappear altogether but rather remained relatively stable at 10-20% frequency until week 50, when new releases (with wMelRio) were initiated. As soon as we observed that Wolbachia frequency plateaued between weeks 7-19, we conducted investigations to elucidate the reasons for such Wolbachia dynamics. In addition, we started doubling the numbers of mosquitoes released per week: from 7,500 in weeks 1-7 to 15,000 in weeks 13-20. However, wMelBr frequency still remained at around 40%.

Bioassays with field populations and Wolbachia strains
The bioassays revealed a significant difference in the insecticide resistance status of wMelBr and wMelRio strains, especially for the pyrethroid deltamethrin. Considering adults, both strains were susceptible to the recently implemented pesticide malathion, but only the wMel-Rio strain could be considered as resistant to deltamethrin (pyrethroids) from the three tested Relevance of genetics to support Wolbachia deployment wild populations (S1 Table and Fig 2A). On the other hand, individuals from the wMelBr population showed a similar trend to the susceptible Rockefeller strain: all individuals exposed to the test concentration of deltamethrin died (S1 Table and Fig 2A). The allelic frequency of kdr mutations of all tested populations reinforces the phenotypic/genotypic response of pyrethroid susceptibility of wMelBr and resistance of wMelRio, as observed in field Ae. aegypti populations (Fig 2B). In the larval assays, all tested populations were susceptible to the newly adopted diflubenzuron. However, both wMelBr and wMelRio strains were diagnosed as resistant to temephos as the field populations (RR 95 > 3,0) (S2 Table and   Relevance of genetics to support Wolbachia deployment Overall, wMelBr and field mosquitoes had, therefore, similar resistance levels to temephos, diflubenzuron and malathion. However, wMelBr was highly susceptible to deltamethrin unlike field populations. When the wMelRio strain was tested, comparable levels of insecticide resistance-susceptibility were found between the wMelRio strain and the three field populations, indicating that the wMelRio-released mosquitoes were as resistant as field Ae. aegypti to the chemical compounds used in the last two decades in Brazil (S1 and S2 Tables).

Frequency of kdr alleles in Tubiacanga
Aedes aegypti from Tubiacanga had a frequency of kdr 'resistant genotypes' (R1R1, R1R2 and R2R2) above 80% during the 84 weeks, which includes the periods of Wolbachia releases, indicating a high level of pyrethroid resistance in the field (Fig 3A). However, field-collected wMelBr mosquitoes retained a high frequency of the wild type allele Na V S, ranging from 60% to 65% up to week 20, while the 'resistant genotypes' did not exceed 20% in the same period ( Fig 3B). Remarkably, a shift in the kdr allelic frequency of Wolbachia-infected mosquitoes was observed after wMelBr releases stopped, as evidenced by a continuous increase in the frequency of Na V R1 and Na V R2 alleles in weeks 26 and 30 and further stabilization up to week 84 ( Fig 3B). Starting at week 30, the kdr allelic frequency was similar between Ae. aegypti with and without Wolbachia. It is of note that the allele Na V R2 significantly increased in wild mosquitoes during wMelBr releases, returning to the same level when wMelBr releases stopped, suggesting a strong selective pressure from pyrethroids in Tubiacanga (Fig 3A).

Insecticide resistance cost in wMelBr
The frequencies of the R2 kdr alleles obtained from samples in the F1 and F18 generations were 60% and 3%, respectively. We generated uniform samples (N = 100,000) for frequencies of the homozygous genotype of the resistant allele (R2R2) within the interval 50-60% and samples of heterozygous frequencies were obtained to have a general frequency of resistant allele at 60% for F1 generation. Estimates showed a fitness cost in the F18 generation at frequencies of 3±1%, generated in a subset of 6490 values. This subset indicates a mean fitness cost of 34% (95% CI: 31-38%).
Oviposition success. The proportion of Ae. aegypti females laying at least one egg varied among populations, ranging from 67.7% (B2) to 86.3% (Field). Egg laying success was strongly affected by mosquito age (χ 2 = 58.01, d.f. = 4, P < 0.001). Female oviposition success was strongly affected by Wolbachia infection, with uninfected mosquitoes more likely to lay at least one egg. Oviposition success dropped more rapidly with age in mosquitoes from the populations with high kdr mutation frequencies (S4 Table).
Fecundity. We observed a slight effect of age on fecundity, with females laying fewer eggs when older in all populations tested. Eggs laid per successful females dropped from 50.5 eggs in the first clutch to 29.8 eggs in the fourth clutch. The number of eggs laid by females with Wolbachia dropped more rapidly than it did in uninfected populations, but bacterial density did not influence clutch size (S5 Table). Importantly, populations with higher frequencies of kdr mutations had a more dramatic decrease in fecundity over time (S5 Table and Fig 4C). Egg hatch. A high level of variation in egg hatching was observed amongst mosquito populations, ranging from 21.1% (4 th clutch of wMelF1CP) to 89.9% (3 rd clutch of Field population), with higher egg hatching in the absence of the Wolbachia infection (Fig 5).
Maternal transmission. From the 249 tested mosquitoes, one female from the F1CP population was not infected with Wolbachia, producing an overall 99.6% rate of infection. From the 1932 individually screened larvae, 1898 (98.2%) were infected. The uninfected female of Relevance of genetics to support Wolbachia deployment  Relevance of genetics to support Wolbachia deployment

Genetic similarities among release and field populations
After removing loci that were absent in more than 25% of individuals from each of the four samples, with minor allele frequency of 5% in at least one sample and having high log-likelihood of heterozygous genotype calls (score � -10), we retained 5,118 SNPs for downstream analysis. We also removed individuals that had more than 30% of missing data across all loci, retaining 20 individuals in each of the four samples: wMelRio, wMelBr, wild (Tubiacanga, Brazil), wildAu (Gordonvale, Australia). The average depth (SE) per locus per individual was 13.5 (5.1) for wMelRio, 17.6 (6.4) for wMelBr, 10.9 (2.3) for wild, and 8.4 (2.1) for wildAu.
Analysis of genetic variation and structuring revealed that the backcrossing strategy achieved a desirable genetic composition of the wMelRio release material. Namely, wMelRio and Tubiacanga were indistinguishable in the analysis of genetic structuring. DAPC analysis showed that these two samples represent one genetic cluster that was clearly differentiated from the wMelBr strain and the wild Australian sample (wildAu) (Fig 6). Genome-wide F ST (Weir and Cockerham 1984) between wMelRio and wild (Tubiacanga) was only 0.02, and this was significantly lower than the F ST values between wMelRio and the wMelBr colony (0.06) or the wild Australian sample (0.114).

wMelRio release in Tubiacanga
wMelRio releases in Tubiacanga started with a 10-20% residual frequency of wMelBr mosquitoes. The release period in Tubiacanga lasted 24 weeks, and Wolbachia frequency reached 80% on the 18 th week after releases started (Fig 1). One year after releases ended, the frequency of Wolbachia remained at 85-95%.

Maintaining insecticide resistance in the colony during wMelRio releases
The frequencies of kdr alleles in the wMelRio strain were quite similar to the field population during the six months (or 10 mosquito generations) in which releases were conducted in Tubiacanga, with Na v R1 frequency being >30% and Na v R2 > 50%. The wild-type Na v S allele was rare, with frequencies consistently lower than 2%. The resistant genotypes (R1R1, R1R2 and R2R2) were above 90% during all releases. The same results were found for field samples during week 34 and 51 (S6 Fig).

Discussion
The endosymbiont Wolbachia has been deployed in endemic areas of several countries as a strategy to reduce dengue, Zika and chikungunya transmission since mosquitoes with this bacterium have significant reduction on their vector competence to arboviruses [9][10][11][12][13][14]. We detailed Wolbachia invasion in Rio de Janeiro and present consistent data on the importance of profiling insecticide resistance of a native population prior to releases to maximize the likelihood of Wolbachia invasion. We also emphasize the importance of proper community engagement and communication, being transparent in all the processes.
Previous releases in places such as North Queensland showed a relatively constant and fast increase of Wolbachia frequency over weeks. For instance, an invasion of 98% was observed on Yorkeys Knob after 12 release weeks [13]. However, in Rio, invasion had plateaued at~40-45% after the same period. Certainly, one of the major forces that may constrain Wolbachia invasion is the wild mosquito population size [41][42]. Tubiacanga has a greater average of mosquito/trap/day than several sites of North Queensland, but slightly smaller than in Tri Nguyen island in Vietnam where Wolbachia releases have also taken place. It is noteworthy that in the first weeks of releases in Rio, the ratio of wild:wMelBr was 1:0.5-0.7, while in North Queensland, a ratio of 1:1.2-1.7 [13,[41][42][43]. Therefore, we initially hypothesized increasing mosquito release numbers would overcome the wMelBr frequency plateau. From week 13 we doubled the number of mosquitoes released, but plateau remained unaltered.
Our second hypothesis was that released mosquitoes were not fit enough to survive in the field, which was likely to be the reason why wMelPop strain releases failed in Tri Nguyen Island in Vietnam and North Queensland [43]. To test this, we measured mosquito wing length, adult survivorship on the first week, and sex ratio as proxies of fitness. In 15 out of 20 weeks, we released females significantly bigger than wild ones, in hope these females would not experience fitness loss due to poor rearing conditions (which was reinforced by the low mortality of adults in release cups). Finally, we checked if there were any deviation in sex ratio in release a cohort, because the ratio biased toward males would likely delay Wolbachia spread as it would rely more on cytoplasmic incompatibility rather than breeding by infected females. However, the sex ratio in our release cohorts was slightly biased towards females. Therefore, the evidence suggests that small wing size, low survival and male-biased sex ratio were not responsible for the plateau in infection frequency.
Another process that can hinder Wolbachia invasion is the loss of its perfect maternal transmission, which can be driven by the high environmental temperature fluctuations during development. Namely, larval exposure to daily fluctuating temperatures (e.g. 26-37˚C or 30-40˚C) during early development dramatically reduced the levels of wMel in emerging Ae. aegypti females [44][45][46]. During Wolbachia deployment in Rio, Tubiacanga experienced harsh climatic conditions, with an unexpected drought summer and a constant increase in temperature starting in week 12 (S1 Fig). Despite extreme temperatures, there was no sign of a high level of incomplete maternal transmission of Wolbachia in Tubiacanga.
The sharp decrease of wMelBr-infected mosquitoes after the end of the first round of releases (Fig 1) raised concerns regarding mating abilities between Wolbachia-released and wild mosquitoes. Acoustic signals produced during flight play an important role in mating success, when wing-beat frequencies between male and female mosquitoes can determine females' rejection/acceptance behaviors toward males [47][48][49]. Recent evidences pointed Wolbachia is unlikely to prevent the harmonic interaction between Ae. aegypti males and females, suggesting released individuals would meet the acoustically-related mating behavior required to promote bacteria invasion [50].
Overall, empirical data allowed us to reject the effect of (a) releasing insufficient numbers of Wolbachia-infected mosquitoes, (b) their reduced fitness, (c) incomplete maternal transmission to explain the plateau and the rapid decrease on wMelBr after the first release ended, and (d) divergent wing-beat frequency of wild mosquitoes and Wolbachia-infected individuals. Then, unexpectedly, the field entomology and CE teams received a clue. The owner of the local grocery store in Tubiacanga mentioned he wished releases never ended, because he was selling insecticide spray cans as never before. This led to an incident report by the CE team which provided the eureka moment for field entomology team. We then started investigating a fourth hypothesis to explain the plateau: lack of pyrethroid resistance in the released mosquitoes.
Based on evidences of the overuse of pyrethroids by local householders for personal protection, we evaluated the dynamics of kdr introgression, a key molecular marker for resistance to pyrethroids, during the backcrossing of wild local males to wMel-infected Ae. aegypti females. The frequency of kdr alleles increased in every backcrossing generation and reached around 80% (60% Na V R2 and 20% Na V R1) (S7 Fig). The knockdown resistance is a recessive trait and the kdr alleles provide a fitness cost in the absence of the selective pressure of insecticide usage [28]. Thus, it is likely that the rapid decrease of kdr alleles in the wMelBr colony was a function of their high fitness cost. There are several reports of fitness cost of pyrethroid resistance on a range of relevant traits such as wing shape in Ae. aegypti [51], and reduction in male mating competitiveness in An. gambiae and Cx. pipiens [52,53]. Overall, fitness costs are expected when the resistance mechanism is related to the alterations in target site, such as kdr mutations. The sodium channel is a key molecule of the nervous system, its alterations generally disturb the normal physiology processes leading to several pleiotropic effects in the overall fitness. There is some evidence indicating that Ae. aegypti mosquitoes have a significant fitness cost for several life history traits due to insecticide resistance, at least under laboratory conditions [54,55]. However, there is still little evidence pointing to costs under field conditions and how resistance genotypes fluctuate depending on insecticide use. Most importantly, here we highlight for the first time, the need to investigate the local insecticide resistance profile before Wolbachia deployment.
The frequency of kdr alleles in Wolbachia positive mosquitoes remained unaltered during the 20 weeks of wMelBr releases. The kdr frequency started changing on week 26 at which time all released mosquitoes were likely dead. In other words, these kdr frequency changes were observed in the offspring of wMelBr released mosquitoes. During the release period, driven by the high selection pressure of household pyrethroid spraying, a small proportion of wMel-infected mosquitoes was able to survive and reproduce due to a low kdr frequency and this kept Wolbachia frequency below the threshold to promote invasion [56]. Remarkably, despite low pyrethroid resistance, wMelBr frequency remained at 10-15% for nine months, when the release of wMelRio started in Tubiacanga. The hypothesis of overuse of pyrethroids spraying by local householders is reinforced by the fluctuation of kdr frequencies in wild mosquitoes ( Fig 3A). The frequency of Na v R2, the allele related with resistance and high fitness cost, increased until the releases stopped. After week 20, the pattern of genotype frequencies shifted and by week 34 it was similar to the one observed before Wolbachia deployment in Tubiacanga.
The main reason for failure of wMelBr invasion in Tubiacanga therefore seems to be related to releasing mosquitoes not fully adapted to local environmental settings. More specific, mosquitoes lacking resistance to pyrethroids. Susceptibility to pyrethroids in a site where wild population is highly resistant to this class of insecticides seems critical but is not the only factor. Adding 10% wild males every five generations to the colony intended for releases was not enough to achieve locally adapted genetic composition of the mosquitoes intended for the releases. High resistance to pyrethroids has spread across Ae. aegypti populations in Brazil [25][26][27]57]. Therefore, to accomplish successful Wolbachia deployment, countries will have to consider generating mosquitoes as resistant as the local wild population. Assessing the insecticide resistance profiles of both the wild population and Wolbachia mosquitoes becomes a key factor in preparation for and during the releases. Wolbachia was able to invade Tubiacanga when released Ae. aegypti had a genetic constitution similar to field mosquitoes. This contrasted to the situation in North Queensland, where there is no evidence for pyrethroid resistance in the field population [58].
Wolbachia-infected Ae. aegypti mosquitoes are currently being deployed in at least ten countries. Due to a worldwide distribution of pyrethroid-resistant alleles, many wild Ae. aegypti populations may have high levels of resistance to pyrethroids [59]. The Ae. aegypti biting rate during Wolbachia deployment increases due to the great influx of released mosquitoes. This disturbance may in the end lead residents to use insecticides more frequently to protect themselves, among which pyrethroids are usually the most available. It will lead to a selection of pyrethroid-resistant individuals of the natural Ae. aegypti population and kill the released Wolbachia-infected if they are not as resistant, precluding the success of invasion. Herein, for the first time, we empirically demonstrate that background knowledge regarding the insecticide resistance status of natural wild Ae. aegypti mosquitoes is critic to achieve Wolbachia usage as a disease transmission control strategy.  Table. Profile of wMelRio and three Ae. aegypti mosquitoes from local field populations (Tubiacanga, Jurujuba and Urca) exposed to two adulticides: (A) the organophosphate malathion (mg/m 2 ) and (B) the pyrethroid deltamethrin (mg/m 2 ) with an exposure of 120 minutes. The dose used to evaluate the resistance ratio of mosquito populations to both insecticides killed all the individuals from the wMelBr and Rock populations. (DOCX) S2 Table. Profile of wMelBr, wMelRio and three Ae. aegypti mosquitoes from local field populations (Tubiacanga, Jurujuba and Urca) exposed to two larvicides: (A) diflubenzuron (μg/L) and (B) the organophosphate temephos (mg/mL). Diflubenzuron is currently employed by the Brazilian Ministry of Health.