A Lentiviral Vector Expressing Japanese Encephalitis Virus-like Particles Elicits Broad Neutralizing Antibody Response in Pigs

Background Japanese encephalitis virus (JEV) is the major cause of viral encephalitis in Southeast Asia. Vaccination of domestic pigs has been suggested as a “one health” strategy to reduce viral disease transmission to humans. The efficiency of two lentiviral TRIP/JEV vectors expressing the JEV envelope prM and E glycoproteins at eliciting protective humoral response was assessed in a mouse model and piglets. Methodology/Principal Findings A gene encoding the envelope proteins prM and E from a genotype 3 JEV strain was inserted into a lentiviral TRIP vector. Two lentiviral vectors TRIP/JEV were generated, each expressing the prM signal peptide followed by the prM protein and the E glycoprotein, the latter being expressed either in its native form or lacking its two C-terminal transmembrane domains. In vitro transduction of cells with the TRIP/JEV vector expressing the native prM and E resulted in the efficient secretion of virus-like particles of Japanese encephalitis virus. Immunization of BALB/c mice with TRIP/JEV vectors resulted in the production of IgGs against Japanese encephalitis virus, and the injection of a second dose one month after the prime injection greatly boosted antibody titers. The TRIP/JEV vectors elicited neutralizing antibodies against JEV strains belonging to genotypes 1, 3, and 5. Immunization of piglets with two doses of the lentiviral vector expressing JEV virus-like particles led to high titers of anti-JEV antibodies, that had efficient neutralizing activity regardless of the JEV genotype tested. Conclusions/Significance Immunization of pigs with the lentiviral vector expressing JEV virus-like particles is particularly efficient to prime antigen-specific humoral immunity and trigger neutralizing antibody responses against JEV genotypes 1, 3, and 5. The titers of neutralizing antibodies elicited by the TRIP/JEV vector are sufficient to confer protection in domestic pigs against different genotypes of JEV and this could be of a great utility in endemic regions where more than one genotype is circulating.


Methodology/Principal Findings
A gene encoding the envelope proteins prM and E from a genotype 3 JEV strain was inserted into a lentiviral TRIP vector. Two lentiviral vectors TRIP/JEV were generated, each expressing the prM signal peptide followed by the prM protein and the E glycoprotein, the latter being expressed either in its native form or lacking its two C-terminal transmembrane domains. In vitro transduction of cells with the TRIP/JEV vector expressing the native prM and E resulted in the efficient secretion of virus-like particles of Japanese encephalitis virus. Immunization of BALB/c mice with TRIP/JEV vectors resulted in the production of IgGs against Japanese encephalitis virus, and the injection of a second dose one month after the prime injection greatly boosted antibody titers. The TRIP/JEV vectors elicited neutralizing antibodies against JEV strains belonging to genotypes 1, 3, and 5. Immunization of piglets with two doses of the lentiviral vector expressing JEV virus-like particles led to high titers of anti-JEV antibodies, that had efficient neutralizing activity regardless of the JEV genotype tested.

Introduction
Mosquito-borne Japanese encephalitis virus is a member of the Flavivirus genus in the Flaviviridae family [1][2][3][4]. Flaviviruses contain a positive single-stranded RNA genome encoding a polyprotein that is processed into three structural proteins, the capsid (C), the precursor of membrane (prM) and the envelope (E), and seven non-structural proteins NS1 to NS5 [4]. Viral assembly occurs in the lumen of the endoplasmic reticulum: the nucleocapsids associate with prM-E heterodimers to form an immature JEV virion. The latter transits through the secretory pathway, where it is matured through cleavage of prM into the membrane (M) protein by furin in the trans-Golgi [4]. Additionally, JEV produces virus-like particles (VLPs), which are assembled solely from prM and E proteins, and undergo the same maturation process as genuine viral particles [5]. These VLPs can be produced in the absence of any other viral component [5].
JEV is the etiologic agent of the most important viral encephalitis of medical interest in South Asia, with an incidence of 50,000 cases and about 10,000 deaths per year [1,3,6]. About 20 to 30% of the symptomatic human cases are fatal, while 30 to 50% of survivors can develop long-term neurologic sequelae. JEV is usually maintained in an enzootic cycle between Culex tritaeniorhynchus mosquitoes and amplifying vertebrate hosts, such as waterbirds and domestic pigs [1,3,7]. Horses and humans are thought to be dead-end hosts, since they do not develop a level of viremia sufficient to infect mosquitoes [7]. In the past decades, there has been an expansion of the geographic distribution of JEV in Asia and a possible introduction of JEV into Europe has been documented recently [6,8]. Phylogenetic studies based on the viral envelope protein sequences allow the division of JEV strains into genotypes G1 to G5 [1,3,[9][10][11][12][13][14][15]. Initially, most of the circulating strains of JEV belonged to G3 and were at the origin of major epidemics in Southeast Asian countries. Recently a shift in prevalence from JEV G3 to G1 has been observed in several Asian countries, while some strains of JEV G5 have been occasionally isolated in China and South Korea [9][10][11][12][13][14][15][16].
We previously demonstrated that both integrative and non-integrative lentiviral vectors are promising vaccination vectors against arboviruses such as West Nile virus (WNV), a neurotropic Flavivirus that belongs to the JEV serocomplex [17,18]. Immunization with a single minute dose of recombinant lentiviral TRIP vectors that express the soluble form of WNV E protein resulted to a robust protection against a lethal challenge with WNV in mice [17,18]. The currently used lentiviral delivery vectors, mostly derived from human deficiency virus-1 (HIV-1), allow in vivo stable transduction of dendritic cells. This allows a sustained antigen presentation through the endogenous pathway, which in turn elicits robust both humoral and cellular adaptative immunity [19][20][21].
Humoral immunity plays a pivotal role in protection against JEV infection [22][23][24][25] and consequently, the elicitation of a protective antibody response is critical in the development of safe JEV vaccines [25]. In the present study, we evaluated the ability of two lentiviral vectors TRIP/ JEV expressing JEV G3 prM and E to induce a protective humoral immune response against JEV infection in a mouse model and in pigs. Both TRIP/JEV.prME vectors were efficient at producing broad anti-JEV neutralizing antibodies in a mouse model. Immunization of piglets with a TRIP/JE vector expressing JEV VLPs elicited high titers of specific neutralizing antibodies that could give a sufficient protection against different JEV genotypes.

Cells and antibodies
Mosquito Aedes albopictus C6/36 cells were maintained at 28°C in Leibovitz medium (L15) supplemented with 10% heat-inactivated fetal bovine serum (FBS). Vero cells and were maintained at 37°C in Dulbecco's modified Eagle medium (DMEM) supplemented with 5% FBS. BHK-21, SK-N-SH, and HEK-293T cells were maintained at 37°C in DMEM supplemented with 10% FBS. Highly purified mouse anti-pan Flavivirus E monoclonal antibody (mAb) 4G2 was produced by RD Biotech (Besançon, France). Mouse mAb anti-JEV NS5 was kindly provided by Y. Matsuura [26]. Antibodies against Calnexin and SNAP-tag were purchased from Enzo Life Sciences and New England Biolabs, respectively. Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG and anti-rabbit IgG antibodies were obtained from Bio-Rad Laboratories. HRP-conjugated goat anti-pig antibody was obtained from Bethyl Laboratories. Alexa Fluor 488-conjugated goat anti-mouse IgG antibody was obtained from Jackson ImmunoResearch.

JEV strains
The JEV G1 strain G1 CNS769_Laos_2009 [27] was kindly provided by R. Charrel [27]. The JEV G3 strain Nakayama was obtained from the National Collection of Pathogenic Viruses (NCPV, Salisbury, UK) and passaged twice on Vero cells. The molecular clone of JEV G3 strain RP-9 was kindly provided by Y-L. Lin [28] and modified to produce pBR322(CMV)-JEV-RP9, as described previously [29]. To produce infectious virus, the molecular clone was transfected into HEK-293T cells using Lipofectamine 2000 (Life Technologies). At three days post-transfection, viral supernatants were collected and used to infect C6/36 cells in order to grow final virus stocks for experiments. The chimera JEV S-g5/NS-g3 (G5/G3) which express the structural proteins C, prM, and E from the JEV G5 strain XZ0934 fused to the nonstructural proteins of JEV G3 RP-9 has been already described [29]. The chimeric JEV S-g1/NS-g3 (G1/ G3) virus containing the structural protein region from the JEV G1 CNS769_Laos_2009 fused to the RP-9 nonstructural protein region was produced as follows. A silent mutation that created a unique restriction site (AflII) at position 2208-2213 (residues 705 and 706 of the viral polyprotein) was introduced directly in pBR322(CMV)-JEV-RP9 through PCR mutagenesis using primers 5'-actgggaaaggctttcacgaccactcttaagggtgctcagagac-3' and 5'-gtctctgagcacccttaagagtggtcgtgaaagcctttcccagt-3' (the Afl II site is underlined). The resulting pBR322(CMV)-JEV-RP9(Afl II) plasmid was used as template to generate the chimeric JEV. The fragment corresponding to nucleotides 114 to 2213 and flanked by the unique sites ApaI and AflII was substituted with the homologous fragments of JEV G1 strain (nucleotides 115-2214). Both chimeric viruses were produced through transfection of the cDNA infectious clone, as described for JEV G3 strain RP-9.

Generation of recombinant lentiviral vectors
For the construction of recombinant lentiviral vectors expressing JEV proteins, modifications that optimize the expression of prM and E genes in mammalian cells were done on the original sequence of JEV strain RP-9 of G3 using a synthetic gene (Genecust, Lux.). The mammalian codon-optimized sequence coding for prM signal peptide followed by prM and E glycoproteins was cloned into the BamH I and Xho I restriction sites of the pTRIPΔU3CMV plasmid, to generate pTRIPΔU3CMV/JEV.prME. The optimized sequence was further modified by mutagenesis PCR to generate pTRIPΔU3CMV/JEV.prME ΔTM that contains the genes encoding prM and E lacking its two transmembrane domains (E ΔTM ). Lentiviral particles were produced by transient calcium co-transfection of HEK-293T cells as described previously [30], but with the following modifications: at 24h hours post-transfection, the cell culture medium was replaced by serum-free DMEM. Supernatants were collected at 48 hours post-transfection, clarified by several rounds of low-speed centrifugation, and stored at -20°C. The recombinant lentiviral vectors were pseudotyped with VSV-G envelope protein of serotype Indiana (IND) or New Jersey (NJ) [31]. In the resulting vectors TRIP/JEV. prME and TRIP/JEV.prME ΔTM the CMV immediate early promoter (CMVie) drives the constitutive expression of recombinant JEV proteins. The TRIP/JEV vector stocks were titrated by real-time PCR on cell lysates from transduced HEK-293T cells and expressed as transduction unit (TU) per ml [17,18]. Titers of non-concentrated TRIP/JEV.prME vector bearing IND or NJ VSV.G envelope protein were 6.8 log 10 TU.mL -1 and 6.2 log 10 TU.mL -1 , respectively.
Titers of lentiviral TRIP/JEV.prME ΔTM vector bearing IND or NJ VSV.G envelope protein were 7.1 log 10 TU.mL -1 and 6.2 log 10 TU.mL -1, respectively. Lentiviral vector stocks were adjusted by dilution in sterile PBS and were inoculated in mice or pigs without further concentration.

Purification of JEV VLPs
Human HEK-293T cells were transduced with recombinant lentiviral vectors. The supernatants were collected at 2 days post-transduction and clarified by centrifugation at 3,000g for 5 min at 4°C. The clarified supernatant was loaded over a 15% sucrose cushion in TNE buffer (10 mM Tris-HCl [pH 7.5], 2.5 mM EDTA, 50 mM NaCl), and centrifuged at 100,000g for 2.5 h at 4°C. The supernatants were discarded, and the purified virus-like particles (VLPs) were suspended in 50 μl of TNE buffer.

Focus-forming assay
For titration of JEV infectivity, focus-forming assays (FFA) were performed on BHK-21 cells as previously described [29]. Virus titers were given in focus forming units per ml (FFU.mL -1 ).

Production of JEV antigens
Large flasks of Vero cell monolayers were inoculated with JEV at low multiplicity of infection. The supernatant fluids of JEV-infected (JEV antigen) or mock-infected (normal cell antigen or NCA) cells were harvested and clarified. The supernatants were precipitated with 7% w / v PEG 6,000 (Fluka), centrifuged, and the viral pellet was suspended in cold PBS supplemented with 0.1% ß-propiolactone in 0.1 M Sorensen buffer (pH 9.0) for JEV inactivation. The working dilution of inactivated JEV antigen (1:200) was estimated based on an « in-house » indirect ELISA using well-characterized human positive JEV serum samples and already validated JEV antigen. The DES expression system (Life Technologies) was required for the production of recombinant viral antigens in Drosophila S2 cells. A synthetic gene coding for prM followed by E ΔTM from JEV G3 strain SA-14 was cloned into the shuttle vector pMT/BiP/SNAP, a derived pMT/BiP/V5-HisA plasmid (Life Technologies) in which the SNAP-tag sequence (Covalys BioSciences AG) had been inserted in frame with the insect BiP signal peptide. The resulting plasmid pMT/BiP/JEV.prME ΔTM -SNAP encodes prM followed by E ΔTM in fusion with the Nterminus of SNAP-tag. The recombinant domain III from the E protein (EDIII) of JEV G1 strain JaNAr0102/Japan/2002/Mosquito, JEV G3 strain GP05, and JEV G5 strain 10-1827 were fused in frame to the C-terminus of SNAP-tag into the plasmid pMT/BiP/SNAP. The resulting plasmids pMT/BiP/JEV.prME ΔTM -SNAP and pMT/BiP/SNAP-JEV.EDIII were transfected into S2 cells to establish stable cell lines S2/JEV.prME ΔTM -SNAP and S2/SNAP--JEV.EDIII for G1, G3, and G5 as previously described [29]. The production and the purification of recombinant viral antigens from stable S2 cell lines were performed as previously described [28].

Immunodetection of viral proteins
Western blot assay was essentially performed as previously described [29]. For immunofluorescence (IF) assay, cells were fixed with 3.2% paraformaldehyde in PBS and permeabilized with 0.1% Triton X-100 in PBS. JEV E protein was detected with the mAb 4G2, followed by incubation with AlexaFluor488-conjugated secondary antibody. The cover slips were mounted with ProLong Gold Antifade Reagent with DAPI (Life Technologies). The slides were examined using a fluorescent microscope (Axioplan 2 Imaging, Zeiss).

Mouse experiments
Groups of 6-week-old female BALB/c mice (n = 6) were intraperitoneally (i.p.) inoculated with recombinant lentiviral vectors in 0.1 ml DPBS supplemented with 0.2% endotoxinfree serum albumin. Animals were bled by puncturing at the retro-orbital sinus level. A very low individual variability exists within each group of mice inoculated with recombinant lentiviral vectors justifying the use of pooled sera in subsequent experiments [18]. A control group of 3-week-old female BALB/c mice (n = 6) was inoculated with 3 log 10 FFU of JEV G3 (strain RP-9) and immune sera was collected and pooled at 21 days postinoculation.
For passive seroprotection experiments, pooled immune sera were transferred i.p. into groups of 3-week-old C57BL/6 female mice (n = 6 or 12) one day before a challenge with 10 FFU of JEV G3 (strain RP-9) inoculated by the i.p. route. The challenged mice were monitored for signs of morbidity and mortality. Euthanasia was applied on animals showing the symptoms of viral encephalitis.

Piglet experiments
Groups of 7-week-old specific pathogen free Swiss Land Race piglets from in-house breeding were housed in groups, and an adaptation time to the new environment of one week was given before starting the experiment.
For immunization, the TRIP/JEV.prME lentiviral vector was diluted to a final volume of 0.5 ml with PBS (Life Technologies). Immunization with the TRIP/GFP lentiviral vector was used as a negative control [18]. From a group of 5 piglets, four were vaccinated intramuscularly with various doses of the TRIP/JEV.prME vector and one was injected with the equivalent dose of control lentiviral vector TRIP/GFP. Immunized animals were bled before the first vaccination and then weekly until the end of the experiment. Four weeks after the first vaccination, all animals got a booster vaccination with the same dose of recombinant lentiviral vectors as at the first time point. For ethical reasons no lethal challenge was performed as protection in pigs. As a control, 3 animals were inoculated by the oronasal route with 7 log 10 TCID 50 of live JEV Nakayama G3. All pigs developed temporary fever and viremia and recovered completely after 4-6 days. The animal sera were examined weekly for anti-JEV antibody.

Indirect ELISA and neutralization test
Indirect ELISA measured the production of anti-JEV IgGs in immunized mice and piglets. A series of 96-well ELISA plates (Nunc) was coated with 0.1 ml of inactivated native JEV antigen or highly purified recombinant JEV antigens diluted in PBS at the concentration of 1 μg.mL -1 at 4°C overnight. NCA and SNAP served as negative control antigens. Indirect ELISA were performed as previously described (29). The Immune Status Ratio (ISR) of each group of immunized mice is obtained by dividing the average of JEV antigen OD 450 values by the average control antigen OD 450 values. The end-point titers of anti-JEV antibodies in mouse sera were calculated as the reciprocal of the last dilution of serum having ISR value > 3.0. Pig sera were tested as described for the mice, using HRP-conjugated goat anti-pig antibody as a secondary antibody. Pig sera obtained prior immunization were used as a negative control.
Neutralizing ability of mouse and pig serum antibodies against JEV was determined by focus (FRNT) or plaque (PRNT) reduction neutralization tests on Vero cells, respectively. Mouse serum samples from each group were pooled. Pig sera were tested individually in triplicates starting at a 1:5 serum dilution. Pooled mouse or individual pig serum were two-fold serial diluted in DMEM supplemented with 2% FBS, with a starting dilution of 1:10, and incubated for 2 h at 37°C with an equal volume of viral suspension containing 100 FFU of JEV. The end-point titer was calculated as the reciprocal of the highest serum dilution tested that reduced the number of FFU (FRNT 50 ) or PFU (PRNT 50 ) by 50%.

Statistical analysis
A Log-rank (Mantel-Cox) test was used to compare survival data. Antibody levels between groups of immunized pigs were compared by Mann Whitney U test and the level of significance was set at 5%. GraphPad Prism (GrapPad Software Inc. La Jolla, CA, USA) was used for all statistical analysis.

Ethics statement
All mice were housed under pathogen-free conditions at the Institut Pasteur animal facility.

TRIP/JEV vectors expressing JEV prM and E
We have reported earlier that a single minute dose of a non-replicative lentiviral vector expressing the soluble form of WNV E glycoprotein induced a robust protective humoral response in a mouse model of WNV encephalitis [17,18]. To assess the potential of lentiviral vectors expressing JEV proteins at eliciting protective humoral response against JEV infection, a mammalian codon-optimized gene encoding prM and E from the JEV strain RP9 of G3 was inserted into the lentivirus TRIP vector (Fig 1). We generated two lentiviral vectors, expressing The codon-optimized sequence encoding prM and E from JEV G3 strain RP-9 was cloned into a TRIP lentiviral vector under the control of human cytomegalovirus immediate early promoter (P CMVie ). The TRIP/JEV.prME vector includes the signal peptide sequence of prM (SP) followed by the entire prM and E gene regions of JEV strain RP-9 of G3. The TRIP/prME ΔTM vector includes the same JEV sequence except that E was deleted from its two transmembrane domains TMD1 and TMD2.
doi:10.1371/journal.pntd.0004081.g001 the prM signal peptide followed by the prM protein and the E glycoprotein, the latter being expressed either in the native form (TRIP/JEV.prME) or lacking its two C-terminal transmembrane domains (TRIP/JEV.prME ΔTM ). In these constructs, prM contributes to the folding, stability, and efficient secretion of the glycoprotein E. Lentiviral vectors which expressed JEV proteins were pseudotyped with VSV-G protein of the IND serotype. Non-replicative TRIP/ JEV.prME and TRIP/JEV.prME ΔTM particles were produced from HEK-293T cells, achieving titers of about 7 log 10 TU.mL -1 .
The antigenicity of recombinant JEV proteins was assessed by transducing HEK-293T cells with the TRIP/JEV.prME or TRIP/JEV.prME ΔTM vectors (Fig 2A). An empty vector served as a control. At 48 h post-transduction, the intracellular form of the E protein was detected using the anti-E MAb 4G2 by IF assay. A similar staining pattern for E was observed in TRIP/JEVtransduced cells expressing prME or prME ΔTM . Immunoblot assays using mouse anti-JEV antisera detected recombinant prM and E in lysates from HEK-293T cells transduced with TRIP/ JEV vectors (Fig 2B). We observed an efficient release of E protein to the supernatants of HEK-293T cells transduced with either TRIP/JEV vectors (Fig 2C, top). The presence of prM was only detected in supernatants from cells transduced with the TRIP/JEV.prME vector (Fig 2C,  top). Because JEV prM and E have the capacity to self-assemble into VLPs, we assessed whether VLPs were secreted from HEK-293T cells transduced with TRIP/JEV vectors. The VLPs were detected by immunoblot assay using anti-E mAb 4G2 and anti-JEV immune serum (Fig 2C,  bottom). Extracellular JEV VLPs containing prM and E accumulated in the supernatant of HEK-293T cells transduced with TRIP/JEV.prME vector but not with TRIP/JEV.prME ΔTM vector. Thus, transduction of cells with TRIP/JEV.prME vector leads to efficient secretion of recombinant JEV VLPs.

TRIP/JEV vectors induce anti-JEV antibody in mice
To evaluate humoral responses induced by the lentiviral TRIP/JEV vectors, adult BALB/c mice were inoculated with increasing doses of TRIP/JEV.prME or TRIP/JEV.prME ΔTM (3 to 5 log 10 TU per animal) by i.p. route. At 21 days post-immunization, sera were collected from each group of mice and pooled. Indirect ELISA was performed to detect anti-JEV IgGs using inactivated JEV particles as capture antigens (Table 1). NCA served as a control antigen. There was little to no antibody responses against JEV at TRIP/JEV vector doses lower than 5 log 10 TU per animal. The dose of 5 log 10 TU induced a significant production of anti-JEV specific antibodies with a mean titer reaching 1,600 for TRIP/JEV.prME and 400 for TRIP/JEV.prME ΔTM (Table 1). At the highest dose (6 log 10 TU) inoculated to mice, the mean titer of TRIP/JEV. prME antibody reached 10,000. The latter dose was not further used due to the too large volume of non-concentrated TRIP/JEV vector inoculated to mice by i.p. route. We therefore decided to select the unique dose of 5 log 10 TU in subsequent mouse immunizations. To determine the time course of anti-JEV production, BALB/c mice that received 5 log 10 TU of TRIP/ JEV.prME or TRIP/JEV.prME ΔTM were bled at 7, 14 and 21 days post-immunization (Table 1). Anti-JEV antibodies were detectable at day 14 of immunization and reached significant titers at day 21.
To enhance the production of anti-JEV specific antibodies, groups of 12 mice previously immunized with 5 log 10 TU of recombinant TRIP/JEV vectors received a booster dose of 5 Times post-immunization 2 (days) TRIP/JEV.prME (10 5 TU) TRIP/JEV.prME ΔTM (10 5 TU) log 10 TU of homologous vectors bearing the VSV-G envelope protein of a different VSV strain (NJ), 4 weeks after the first inoculation. Immune sera were collected 3 weeks after the boosting inoculation and ELISA measurements on pooled sera showed a 40-fold increase in anti-JEV antibody titers reaching the mean titers of 64,000 for TRIP/JEV.prME and 16,000 for TRIP/ JEV.prME ΔTM . It is of interest that the level of anti-JEV antibody in mice twice immunized with 5 log 10 TU of recombinant lentiviral vector expressing JEV VLPs was similar to that obtained following a challenge with 3 log 10 FFU of live JEV G3 RP-9.

Reactivity of anti-TRIP/JEV antibodies against JEV prM and E
The reactivity of antibodies raised in mice after TRIP/JEV immunization was evaluated by immunoblotting using lysates of JEV-infected cells as viral antigens (Fig 3). Mice that received TRIP/JEV.prME displayed specific antibodies against prM and E whereas TRIP/JEV.prME ΔTM antisera contained only anti-E antibody. Thus, the co-expression of prM and the soluble form of E failed to stimulate the production of anti-prM antibody in a mouse model. Indirect ELISA tests were performed to determine the anti-E antibody levels in sera from mice immunized with TRIP/JEV.prME or TRIP/JEV.prME ΔTM . The soluble form of JEV G3 E fused to SNAP tag (JEV.E ΔTM -SNAP) was used as a capture antigen of anti-JEV E IgGs. Given that flavivirus EDIII contains sub-type specific neutralizing epitopes, the recombinant SNAP-tagged EDIII proteins of G1, G3, and G5 were also used as capture viral antigens. Sequence alignment of the three EDIII identified a similarity of 96.3% between G3 and G1 and 92.5% between G3 and G5 ( Fig 4A). Purity and specificity of recombinant JEV.E ΔTM -SNAP protein ( Fig 4B) and SNAP--JEV.EDIII of G1, G3, and G5 protein ( Fig 4C) were verified by immunoblotting using an antibody against SNAP-tag. Indirect ELISA showed that BALB/c mice that recovered from a lethal challenge with JEV G3 gave titers of anti-E antibody of 1,300 and anti-EDIII antibody from 1,000 to 4,000 (Table 2). BALB/c mice that received two doses of TRIP/JEV.prME ΔTM or TRIP/ JEV.prME elicited anti-E antibody titers with a similar range of about 1,000. Both lentiviral TRIP/JEV vectors were capable of inducing a similar level of anti-EDIII antibodies that are broadly reactive with different genotypes of JEV. It is of interest that immunization with TRIP/ JEV vectors induced higher levels of anti-EDIII G5 antibody than live JEV G3. In vitro cross-neutralizing activity of anti-TRIP/JEV antibody A focus reduction neutralization test (FRNT50) was performed to evaluate the ability of TRIP/ JEV vectors to elicit a neutralizing antibody response against JEV G3 (Table 3). Immune sera obtained from BALB/c mice that recovered from a lethal challenge with JEV strain RP-9 had a FRNT 50 of 150. A weak FRNT 50 titre of 10 was observed in mice inoculated with a single dose  . Immune sera were collected from groups of 3-week-old BALB/c mice (n = 6) at 21 days after intraperitoneal inoculation with 3 log 10 FFU of JEV G3 (strain RP-9). 3 . Immune sera were collected from groups of 6-week-old BALB/c mice (n = 6) at 21 days after intraperitoneal inoculation with 5 log 10 TU of lentiviral vectors. doi:10.1371/journal.pntd.0004081.t002 Lentiviral Vector-Based JEV Vaccine of 5 log 10 TU of TRIP/JEV vector. A booster dose one month after the prime elicited JEV-neutralizing antibodies titers to 40 (TRIP/JEV.prME ΔTM ) and 80 (TRIP/JEV.prME) ( Table 3). Recent studies addressed the ability of already existing G3 derived vaccines at protecting against strains belonging to distinct genotypes [32][33][34], and such testing should be systematically included when assessing newly designed JEV vaccines. We assessed the protective capacity of TRIP/JEV immunization against other circulating JEV genotypes, namely G1 and G5. To investigate this issue, we decided to substitute the region encoding C, prM and E into the infectious cDNA clone of JEV G3 by the counterpart from JEV G1 or G5 (Fig 5A). Since immunizations with the TRIP/JEV vectors are solely directed against JEV structural proteins, the contribution of non-structural proteins of JEV G1 and G5 was not explored. The growth of chimera JEV G1/3 or JEV G5/3 was comparable to that of parental JEV virus of G3 in cultured cell lines [29]. Immunoblot analysis showed that JEV G3 antisera recognized both prM and E from JEV G1 and G5 (Fig 5B, left panel). However, JEV G3 antisera weakly reacted with prM from the chimera JEV G5/G3. Essentially similar results were obtained when immunoblot assay was performed with anti-TRIP/JEV antisera (Fig 5B). The immune sera from BALB/c mice inoculated with TRIP/JEV.prME or TRIP/JEV.prME ΔTM recognized the E protein from chimera JEV G1/G3 and G5/G3. Only TRIP/JEV.prME antisera reacted with prM from chimera JEV G1/G3 and a to lesser extent, JEV G5/G3. This lower reactivity was not due to a defect in JEV G5/3 protein accumulation since the reactivity of anti-JEV NS5 antibody was comparable amongst the different lysates of JEV-infected cells (Fig 5B, lower right panel). We observed that TRIP/ JEV.prME ΔTM was capable of inducing antibodies that can similarly react with the E protein from chimera JEV G1/3 and G5/3, thus suggesting that soluble E ΔTM exhibits a greater propensity to generate anti-E antibodies that recognize conserved epitopes regardless of the genotype (Fig 5B).
FRNT assays were performed to evaluate the ability of TRIP/JEV vectors to elicit a neutralizing antibody response against JEV G1/3 or G5/3 (Table 3). Pooled immune sera obtained from BALB/c mice infected with JEV G3 gave had FRNT 50 values of 140 and 50 against chimeric JEV G1/3 and G5/3, respectively. Immunized mice that received TRIP/JEV vectors developed neutralizing antibody that were also active against chimeric JEV G1/3 and G5/3 (Table 3). We noted that immunization with the TRIP/JEV.prME vector elicited slightly higher levels of neutralizing anti-JEV antibodies. The lower neutralization capability of TRIP/JEV-induced antibodies against chimera G5/3 correlated with the weaker reactivity of immune sera towards the . The neutralization activities of pooled immune sera against three live JEV [G3 (strain RP-9), G1/3 chimera (strains CNS769_Laos_2009 and RP-9) and G5/3 chimera (strains XZ0934 and RP-9)] were evaluated using a standard FRNT assay in Vero cells. FRNT 50 values representative of n = 3 independent experiments are given. 2 . Immune sera were collected from groups of 3-week-old BALB/c mice (n = 6) at 21 days after intraperitoneal inoculation with 3 log 10 FFU of JEV G3 (strain RP-9). 3 . Immune sera were collected from groups of 6-week-old BALB/c mice (n = 6) at 21 days after intraperitoneal inoculation with 5 log 10 TU of lentiviral TRIP/JE vectors. doi:10.1371/journal.pntd.0004081.t003 prM and E proteins from JEV of G5 ( Fig 5B). These data show that both TRIP/JEV vectors were capable of stimulating the production of anti-JEV antibodies that neutralize JEV G1 and G3, and to a lesser extent JEV G5.

Passive transfer of TRIP/JEV antisera partially protects mice from JEV infection
We previously reported that inoculation of JEV G3 strain RP-9 to three-week-old C57BL/6 mice was lethal within one week [28]. Given that mouse susceptibility to JEV is age-dependent, we were unable to challenge animals following the long prime-boost vaccination period with TRIP/JEV vectors. Consequently, we decided to apply a protocol of passive transfer of TRIP/ JEV antisera into three-week-old C57BL/6 mice. To address whether the humoral immunity elicited in mice after TRIP/JEV.prME or TRIP/JEV.prME ΔTM vaccination was protective in vivo, groups of 3-week-old C57BL/6 mice received i.p. inoculation of 10 μl of pooled, heat-inactivated immune sera collected from TRIP/JEV-inoculated mice two months after boosting. Pooled immune sera of BALB/c mice inoculated with JEV G3 served as a positive control. A group of mice inoculated with PBS was included. One day after the passive transfer of antisera, the mice were i.p. challenged with a lethal dose of JEV G3. The animals were observed daily for clinical signs of illness and mortality over three weeks (Fig 6). Approximately 70% of the mice inoculated with PBS died within the 9-11 days post-challenge whereas administration of JEV immune sera induced a survival rate of 85% (P = 0.05). A survival rate of 50-60% was observed in mice after transfer of TRIP/JEV.prME or TRIP/JEV.prME ΔTM antisera. While inoculation with a single dose of TRIP/JEV antisera did not confer satisfactory levels of protection in our mouse model, we do note that a slight level of protection was obtained.
TRIP/JEV.prME induces the production of neutralizing anti-JEV antibodies in pigs Because lentiviral based-expression of JEV VLPs is particularly efficient at triggering neutralizing antibody responses, we assessed the capacity of TRIP/JEV.prME to stimulate a protective humoral response in pigs. Groups of four 7-week-old piglets were immunized intramuscularly with 6 (low dose) or 7 (high dose) log 10 TU of TRIP/JEV.prME (Fig 7). As a control, two animals received a low or high dose of a recombinant lentiviral vector expressing reporter GFP. Indirect ELISA using recombinant E ΔTM -SNAP protein as a viral antigen was used to assess the production of anti-JEV E antibodies in immunized pigs weekly (Fig 7A). The monitoring of the antibody responses during the first 4 weeks after the prime inoculation revealed an efficient production of anti-JEV E antibodies. Comparison of the low and high dose immunization did not show statistically significant differences in anti-JEV E antibody production over this time period. The levels of anti-JEV E antibodies was enhanced after the boost performed on week 4, and reached a plateau at least 1.5 month after the prime. When compared to the low dose, the high dose of TRIP/JEV.prME was more effective at eliciting a high level of specific antibody production (P = 0.028). As shown in the Fig 7B, the anti-JEV antibody titers induced 3 weeks after experimental infection of pigs with a single dose of live JEV were comparable to those stimulated in animals by a prime/boost immunization with 7 log 10 TU of TRIP/JEV.prME lentiviral vector. The isotyping of anti-JEV E antibodies showed that TRIP/JEV.prME stimulated the production of both IgG1 and IgG2 by 2 weeks after the prime, and was followed by a decline at week 3 even at the high dose (Fig 7C and 7D). The levels of both anti-JEV E IgG1 and IgG2 were Anti-JEV IgG responses of piglets immunized with TRIP/JEV.prME. In (A), two groups of four piglets were immunized intramuscularly with 6 (low dose) or 7 log 10 TU (high dose) of TRIP/JEV.prME. As a control, two animals were inoculated with either low or high dose of TRIP/GFP. Animals were boosted 4 weeks after primary immunization with the same initial dose (vertical arrow). Serum samples were collected weekly and tested at a dilution of 1:400 for the presence of anti-JEV E IgGs by indirect ELISA. In (B), a group of three animals were experimentally infected with JEV strain Nakayama. The immune sera were tested at a dilution of 1:400 for the presence of anti-JEV E IgGs by indirect ELISA. In (C, D), box plots of the anti-JEV E IgG1/IgG2 from 1 to 10 weeks after immunization with the low (C) or high (D) dose of TRIP/JEV.prME are depicted. The vertical arrow indicates the boost. In (E), the levels of anti-JEV E IgG1/IgG2 in immune sera from piglets infected with JEV strain Nakayama.
doi:10.1371/journal.pntd.0004081.g007 similar to those observed in piglets challenged with JEV strain Nakayama at the week 3 of infection ( Fig 7E). In animals primed with TRIP/JEV.prME, the boost at week 4 enhanced preferentially the production of IgG2 by 10 weeks after the prime regardless of the inoculated dose.
The individual serum samples obtained from animals immunized with the lentiviral TRIP/ JEV.prME vector were also examined for neutralizing antibodies at 3 weeks after the prime and at 6 weeks after the boost (Fig 8). Immunized piglets that received a single dose of 6 to 7 log 10 TU of TRIP/JEV.prME developed neutralizing antibody titers ranging from 10 to 30 against the homologous JEV G3 strain RP-9 and reached titers up to 160 after the boost (Fig 8A). The higher dose of TRIP/JEV.prME induced a stronger anamnestic neutralizing antibody response.
Examination of the piglet immune sera revealed that, regardless of the inoculated dose, TRIP/JEV.prME elicited neutralizing antibodies against the Nakayama strain of JEV G3, the strain XZ0934 (tested using the JEV G5/G3 chimera) of JEV G5 and, to a lesser extent, the strain CNS769_Laos_2009 of JEV G1 (Fig 8B). Importantly, the pattern of neutralizing activity of anti-TRIP/JEV.prME antibody was similar to that observed in immune sera collected from a group of piglets experimentally infected with the JEV strain Nakayama (Fig 8C).
These results showed that TRIP/JEV.prME is able to elicit high titers of neutralizing antibodies in piglets that received two inoculations with 7 log 10 TU of lentiviral vector with an interval of one month. Additionally, we found that TRIP/JEV.prME is capable of stimulating the production of anti-JEV antibodies that neutralize JEV G1 and G5.

Discussion
Several vaccines against JEV are currently available to humans and for some animals such as horses and pigs: those are inactivated mouse brain-derived, inactivated cell culture-derived, live-attenuated and chimeric yellow fever virus-JEV vaccines [4,7,23,[32][33][34][35][36][37][38][39][40][41][42][43][44]. However, some of them lack induction of long-term immunity and live-attenuated vaccine strains carry a possible risk of reversion to virulence [4]. Also the cost effectiveness of JEV vaccines is considered Neutralizing antibody response in piglets immunized with TRIP/JEV.prME. Sera from piglets immunized with a low or high dose of TRIP/JEV. prME were tested for neutralization ability against JEV by PRNT50. In (A), the piglet sera collected prior immunization, 3 weeks after priming or 6 weeks after the boost were tested against the JEV G3 strain RP-9. In (B), the TRIP/JEV.prME antisera collected after the boost were tested for their cross-neutralizing capacity against JEV G1 and G3 strains, and JEV chimera G5/G3. In (C) the neutralizing activity of anti-JEV antibodies from animals experimentally infected with JEV G3 strain Nakayama was tested against JEV G1, G3, and the JEV chimera G5/G3 by PRNT50.
doi:10.1371/journal.pntd.0004081.g008 as a major obstacle [7]. DNA vaccines were shown to be as successful as commercial live-attenuated vaccines at eliciting anti-JEV immune responses in a mouse [25] and non-human primate models [44]. While those vaccines were designed to express the prM and E proteins, the fact that they were DNA vaccines required greater production costs.
Lentiviral vectors represent a novel and attractive platform for gene-based immunization [20]. The ability of lentiviral vectors to efficiently transduce non-dividing dendritic cells allows a prolonged antigen presentation through the endogenous pathway, which in turns translates into the induction of strong, multi-epitopic and long lasting humoral as well as cellular immune responses. Consequently, an increasing number of pre-clinical studies show a great vaccine efficacy of lentiviral vectors in both infectious diseases and anti-tumor vaccination fields [19-21, 31, 45-47].
The purpose of our study was to evaluate the ability of two lentiviral TRIP-based vectors expressing the prM and E proteins from JEV, namely the TRIP/JEV.prME and TRIP/JEV. prME ΔTM vectors, at eliciting protective humoral immune response in mice and piglets. We showed that JEV VLPs accumulated in the supernatant of cells transduced with TRIP/JEV. prME but not TRIP/JEV.prME ΔTM . Thus TRIP/JEV.prME expresses recombinant prM and E that are secreted efficiently, which represents a supplementary asset in the ability at eliciting anti-JEV antibody response. Mice inoculated with a single low dose (5 log 10 TU) of TRIP/JEV vectors developed JEV-specific IgGs and a booster dose one month after the prime resulted in a 40-fold increase in anti-JEV antibody titers. The reactivity of anti-JEV antibodies was documented by indirect ELISA and immunoblot assays. Mice immunized with TRIP/JEV.prME developed both anti-E and anti-prM antibodies whereas only anti-E antibodies were detected in sera of mice immunized with TRIP/JEV.prME ΔTM . The E protein acts as the main target for imparting protective immunity against JEV-related disease [23,32], and its antigenic domain EDIII contains important epitopes that are recognized by neutralizing antibodies [48]. Further analysis of the recognition of JEV antigens by TRIP/JEV antisera showed that immunization with either TRIP/JEV.prME or TRIP/JEV.prME ΔTM generated comparable levels of antibodies against the E protein, as well as type-specific epitopes located in its antigenic EDIII domain. Immunization with TRIP/JEV vectors induced neutralizing antibodies against JEV belonging to genotypes, G1, G3, and G5. Such observation is of particular importance at the period where it is evidenced that G1 JEV strains are replacing G3 strains [14], from which most vaccines were designed.
It is widely accepted that the humoral immune response is an essential component of protective immunity against JEV infection [23,24]. Consistent with the notion that VLPs are suitable as vaccine against arboviral disease including Japanese encephalitis [5,49], TRIP/JEV. prME was the more efficient lentiviral vector in the production of neutralizing anti-JEV antibodies that conferred partial protection after their passive transfer in mice challenged with JEV. Inoculation of two doses of 7 log 10 TU with a one-month of interval of TRIP/JEV.prME vector in piglets was highly efficient at eliciting high titers of anti-JEV neutralizing antibody that are potentially able to protect pigs from JEV infection. TRIP/JEV.prME was capable of stimulating the production of anti-JEV antibodies that neutralize JEV G3 and G5, and, to a lesser extent, G1. The potential impact of JEV genotype change on vaccine potency has been estimated and immune sera obtained from pigs injected with a G3 vaccine showed lower strain-specific cross-neutralizing antibody titers against JEV of G1 [36]. Such observation led to the development of new veterinary vaccines for pigs specifically directed against this particular genotype of JEV [43]. Although in our hands the TRIP/JEV.prME vector elicited neutralizing antibodies against a G1 virus in pigs, we did note that their levels were lower when compared to the other JEV genotypes tested. However, neutralizing antibodies titers against JEV of G1 could reach 1:40, and thus could be sufficient to achieve protection in pigs. It could be nevertheless important to design alternate JEV antigen with a broader cross-reactivity against JEV strains of G1.
In this study, we demonstrated that immunization of pigs with a TRIP/JEV vector expressing JEV VLPs is particularly efficient at priming antigen-specific humoral immunity and triggers neutralizing antibody responses against the genotypes 1, 3, and 5 of JEV. The production of virus neutralizing antibodies is critical to protection against JEV infection in pigs [50] and a titer at least 1:10 is indicative of protective humoral immunity [51]. The titers of neutralizing antibodies elicited by the lentiviral TRIP/JEV.prME vector are sufficient to confer protection in domestic pigs against different genotypes of JEV and this could be of a great utility in endemic regions where more than one genotype circulates.