Targeting the Wolbachia Cell Division Protein FtsZ as a New Approach for Antifilarial Therapy

The use of antibiotics targeting the obligate bacterial endosymbiont Wolbachia of filarial parasites has been validated as an approach for controlling filarial infection in animals and humans. Availability of genomic sequences for the Wolbachia (wBm) present in the human filarial parasite Brugia malayi has enabled genome-wide searching for new potential drug targets. In the present study, we investigated the cell division machinery of wBm and determined that it possesses the essential cell division gene ftsZ which was expressed in all developmental stages of B. malayi examined. FtsZ is a GTPase thereby making the protein an attractive Wolbachia drug target. We described the molecular characterization and catalytic properties of Wolbachia FtsZ. We also demonstrated that the GTPase activity was inhibited by the natural product, berberine, and small molecule inhibitors identified from a high-throughput screen. Furthermore, berberine was also effective in reducing motility and reproduction in B. malayi parasites in vitro. Our results should facilitate the discovery of selective inhibitors of FtsZ as a novel anti-symbiotic approach for controlling filarial infection. Note The nucleotide sequences reported in this paper are available in GenBank™ Data Bank under the accession number wAlB-FtsZ (JN616286).


Introduction
Filarial nematode parasites are responsible for a number of devastating diseases in humans and animals. These include lymphatic filariasis and onchocerciasis that afflict 150 million people in the tropics and threaten the health of over one billion. Unlike other nematodes, the majority of filarial species are infected with an intracellular bacterium, Wolbachia [1]. In the human filarial nematode Brugia malayi, these obligate a-proteobacterial endosymbionts have been detected in all developmental stages [2][3][4]. Moreover, their presence is essential for the worm, as tetracyclinemediated clearance of bacteria from Brugia spp. leads to developmental arrest in immature stages and reduction in adult worm fertility and viability [5][6][7][8][9][10]. These findings have pioneered the approach of using antibiotics to treat and control filarial infections. However, in humans, tetracycline therapy is not ideally suited for widespread use because several weeks of treatment are required and the drug has contra-indications for certain individuals. Therefore, there is considerable interest in identifying new endosymbiont drug targets and other classes of compounds with anti-Wolbachia activity. Importantly, the completed genome sequence of the Wolbachia endosymbiont of B. malayi (wBm) [11] now enables genome-wide mining for new drug targets [11][12][13][14] and a foundation for rational drug design. These approaches should lead to the discovery of new classes of compounds with potent anti-Wolbachia/antifilarial activ-ities targeting essential processes that are absent or substantially different in the mammalian host.
Bacterial cytokinesis has emerged as a major target for the design of novel antibacterial drugs [15][16][17] since several of the components that are essential for multiplication and viability are absent from mammals. The bacteria-specific ''filamenting temperature sensitive'' protein, FtsZ, plays a central role during bacterial cytokinesis. In Escherichia coli, temperature sensitive mutations in the ftsZ gene cause blockage in cell division with limited cell growth and the generation of long filaments. FtsZ assembles into the contractile Z-ring and coordinates more than a dozen other cell division proteins at the midcell site of the closing septum [18][19][20][21]. Formation of the septal Z-ring requires two important functional properties of FtsZ, namely, polymerization of the FtsZ monomers into protofilaments and GTPase activity. Since inhibition of either function is lethal to bacteria, both GTPdependent polymerization [22][23][24][25][26][27] and enzymatic [27][28] activities of FtsZ have been targeted for the identification of new antibacterial agents. Several inhibitors have been discovered including synthetic compounds [17,29] and natural products [17,[30][31][32][33].
In the present study, we identify the cell division machinery present in wBm and characterize the FtsZ protein (wBm-FtsZ). Using quantitative real time RT-PCR, Wolbachia ftsZ was found to be expressed throughout the life cycle, but up-regulated in fourth stage larvae and adult female worms. Recombinant wBm-FtsZ was shown to possess a robust GTPase activity, which was inhibited by the natural plant product berberine. Berberine was also effective in reducing motility and reproduction in B. malayi parasites in vitro. A library of small molecules was also examined for its inhibitory activity against the wBm and E. coli FtsZ proteins. Several compounds were identified as potent inhibitors, and structureactivity relationship studies revealed a derivative with selectivity for wBm-FtsZ. Thus, our results support the development of wBm-FtsZ as a promising new drug target in an anti-symbiotic approach for controlling filarial infection.

Materials and Methods
Cloning of ftsZ from the Wolbachia endosymbiont of the human filarial parasite B. malayi (wBm-ftsZ) Living B. malayi adult female worms were purchased from TRS Laboratories, Athens GA. Genomic DNA and RNA were isolated following the protocols developed by Dr. Steven A. Williams (http://www.filariasiscenter.org/molecular-resources/protocols).
To clone full-length wBm-ftsZ for expression studies, forward 59(GAGAGCTAGCATGTCAATTGACCTTAGTTTGCCAG)39 (NheI site underlined) and reverse 59(GAGACTCGAGTTACTT-CTTTCTTCTTAAATAAGCTGG) 39 (XhoI site underlined) primers were designed according to the wBm-ftsZ sequence (accession number: YP_198432) in order to amplify the gene from B. malayi genomic DNA. The PCR product was then cloned into the NheI and XhoI sites of pET28a(+) (Novagen) to generate a fusion protein with a His 6 tag at the N terminus. The authenticity of the insert was verified by sequencing.
Wolbachia ftsZ gene expression in various developmental stages of B. malayi Total RNA supplied by the Filariasis Research Resource Center (FR3) was treated with RNase-free Dnase (New England Biolabs, Cat# M0303S) and purified using the RNeasy Kit from Qiagen. cDNA was obtained using random primers and the ProtoScriptH AMV First Strand cDNA Synthesis Kit (New England Biolabs, Cat# E6550S). Forward primer 59 (AACAAGAGAGGCAAGAGCTG-GAGT) and reverse primer 59(CGCACACCTTCAAAGCCAAA-TGGT) were utilized to amplify a 102 bp Wolbachia ftsZ amplicon. Wolbachia 16S rRNA amplified with forward primer 59 (TGA-GATGTTGGGTTAAGTCCCGCA) and reverse primer 59(AT-TGTAGCACGTGTGTAGCCCACT) was utilized for bacterial total RNA quantification. B. malayi 18S rRNA amplified with forward primer 59 (ACTGGAGGAATCAGCGTGCTGTAA) and reverse primer 59(TGTGTACAAAGGGCAGGGACGTAA) was utilized as a total worm RNA control. Quantitative PCR was performed using the DyNAmo TM HS SYBRH Green qPCR Kit (Thermo Fisher) and a CFX-96 Real Time PCR instrument (Bio-rad, Hercules, CA). Relative levels of ftsZ expression (ratio of ftsZ to 16S rRNA), and abundance of Wolbachia in B. malayi (ratio of Wolbachia 16S to B. malayi 18S rRNA) were calculated for each RNA sample. Experiments were performed twice with triplicate samples. Controls consisting of samples processed in the absence of reverse transcriptase were included in qPCR and no DNA contamination was detected.

Identification and cloning of FtsZ from the Wolbachia endosymbiont of Aedes albopictus
To determine the sequence of the ftsZ gene from the Wolbachia endosymbiont wAlB present in the insect cell line Aa23 [34], multilocus sequence typing (MLST) ftsZ forward 59 (TGTA-AAACGACGGCCAGTATYATGGARCATATAAARGATAG) and reverse 59 (CAGGAAACAGCTATGACCTCRAGYAATG-GATTRGATAT) [35] primers were utilized to obtain a PCR fragment. Using BLAST analysis, the sequence of the PCR product was compared to the corresponding region of known fulllength ftsZ sequences and their conserved downstream and upstream sequences and 6 additional primers 59(TCTATTTT-TAATTCTTTTAGAGAAGCATT), 59(CGTTCGGTTTTGA-AGGTGTGC), 59 (ACCGTTGTGGGAGTGGGTGGT), 59 (T-TATTTTTTTCTTCTTAAATAAGCTGGTATATC), 59 (GG-AATGACAATAAGTGTATCTACGTA), and 59(TGCATTTG-CAGTTGCTCATCC) were designed to obtain a complete wAa-ftsZ sequence. PhusionH High-Fidelity DNA Polymerase (New England Biolabs, M0530) was utilized for all PCR reactions according to manufacturer's instructions.

Expression and purification of recombinant Wolbachia
FtsZ proteins wBm-ftsZ and E. coli ftsZ (Ec-ftsZ) were amplified using genomic DNA isolated from B. malayi and E. coli wild-type strain MG1655 respectively, and were then cloned into the pET28a plasmid to generate fusion proteins with a N-terminal His tag. Each protein was expressed in the Escherichia coli strain C2566 (New England Biolabs). Optimum conditions for production of soluble recombinant wBm-FtsZ involved co-transformation with the pRIL plasmid isolated from BL21-CodonPlus (DE3) cells (Stratagene) together with the pET28a-ftsZ plasmid. Cultures were grown at 37uC till the OD 600 reached 0.6, before induction with 0.1 mM IPTG overnight at 16uC. Both Ec-FtsZ and wBm-FtsZ were purified using a similar method. The cells expressing the recombinant proteins were suspended in lysis buffer (20 mM NaPO 4 , 500 mM NaCl, 10 mM imidazole, pH 7.4) plus 1 mg/mL lysozyme and protease inhibitor cocktail (Roche) and incubated on ice for 30 min, followed by sonication. The lysate was then cleared by centrifugation at 12,500 rpm, 4 uC for 30 min. The His-tagged proteins were purified on a 5 mL HiTrap chelating HP column (GE Healthcare) using an AKTA FPLC following manufacturer's instructions. After application of the sample, the column was washed with 5 column volumes of buffer A (20 mM NaPO 4 , 500 mM NaCl, 10 mM imidazole, pH 7.4) followed by 10 column volumes of 92% buffer

Author Summary
Filarial nematode parasites are responsible for a number of devastating diseases in humans and animals. These include lymphatic filariasis and onchocerciasis that afflict 150 million people in the tropics and threaten the health of over one billion. The parasites possess intracellular bacteria, Wolbachia, which are needed for worm survival. Clearance of these bacteria with certain antibiotics leads to parasite death. These findings have pioneered the approach of using antibiotics to treat and control filarial infections. In the present study, we have investigated the cell division process in Wolbachia for new drug target discovery. We have identified the essential cell division protein FtsZ, which has a GTPase activity, as an attractive Wolbachia drug target. We describe the molecular characterization and catalytic properties of the enzyme and demonstrate that the GTPase activity is inhibited by the natural product, berberine, and small molecule inhibitors identified from a high-throughput screen. We also found that berberine was effective in reducing motility and reproduction in B. malayi parasites in vitro. Our results should facilitate the discovery of selective inhibitors of FtsZ as a novel antibiotic approach for controlling filarial infection.
Fractions containing wBm-FtsZ or Ec-FtsZ were pooled, dialyzed against dialysis buffer (40 mM Tris-HCl, 200 mM NaCl and 50% glycerol, pH 7.5) and stored at 220uC prior to use. Purity of the proteins was estimated by 4-20% SDS-PAGE and the protein concentration was determined using the Bradford assay.

GTPase enzyme assay
GTPase activity was measured using an enzyme-coupled assay [36]. Activity was determined by measuring the consumption of NADH, which is monitored by absorbance at 340 nm. The amount of NADH oxidized to NAD corresponds to the amount of GDP produced in the reaction. Reactions were optimized for a 96-well format to enable compound screening. The 100 mL reaction mixture containing 50 mM MOPS (4-morpholinepropanesulfonic acid) pH 6.5, 50 mM KCl, 5 mM MgCl2,1 mM PEP, 500 mM NADH, 0.1% Tween-20, 20 units/mL of L-lactate dehydrogenase (Sigma L2518) and pyruvate kinase (Sigma P7768), 1 mM GTP and 5 mM FtsZ was distributed into 96-well plates. The plate was incubated at 30 uC for 45 min with data collected at 20 second intervals using a SpectraMaxH Plus 384 (Molecular Devices) spectrophotometer. Control assays without FtsZ were performed to provide a baseline and with GDP to ensure the function of the coupling enzymes.
For inhibitor screening, 100 mL of reaction mixture was added to each well of a 96-well plate and 1 mL of compound dissolved in DMSO, or berberine sulfate (MP Biomedicals) in water, in varying concentrations were added. The reaction was initiated at 30 uC by adding 1 mM GTP. Experiments were performed in triplicate.

Effect of berberine on B. malayi
Living B. malayi adult female and male worms were washed extensively with RPMI1640 medium supplemented with 2 mM glutamine, 10% Fetal Calf Serum (Gibco) and 100 U/mL streptomycin, 100 mg/mL penicillin, 0.25 mg/mL amphotericin B (Sigma). Three worms of either gender were distributed into each well of a 6-well plate and incubated at 37 uC, 5% CO 2 . After overnight recovery, motility and microfilaria production were recorded. Worms were then transferred to a new well containing varying amounts of berberine sulfate dissolved in water, namely 40 mM, 20 mM, 10 mM and 5 mM. Control wells containing either no drug or 10 mM doxycycline, were also included. Culture media were replaced with fresh medium containing drug daily. Adult worm and microfilaria motility production were recorded daily as described [37]. Motility was scored as described [38] and expressed as % of motility relative to motility scored on day 0 of the experiment. Microfilaria production was counted in 10 mL of either diluted or concentrated culture medium using a hemocytometer. The results were presented as the number of microfilaria released in 1 mL of medium from each well on the indicated day. Each treatment was performed in triplicate and the experiment was repeated several times.

Effect of berberine on E. coli growth
Berberine sulfate (MP Biomedicals) was added at a final concentration of 0-400 mM to growth medium containing E. coli ER1613 (acrA13 D(top-cysB)204 gyrB225 IN(rmD-rmE) mcrA) (New England Biolabs) and growth determined during 5 h or 20 h of incubation. For the 5 h evaluation, an overnight culture of E. coli ER1613 (acrA13 D(top-cysB)204 gyrB225 IN(rmD-rmE) mcrA) (New England Biolabs) was diluted 100-fold and 1 mL volumes were dispensed into a 48-well deep well plate (Axygen Scientific) containing various concentrations (0-400 mM) of berberine sulfate (10 mL of serial diluted berberine sulfate in water). The plate was then incubated at 30 uC with shaking. After 90 min of initial growth, bacterial growth was determined every 30 min for 5 h by monitoring absorption at 600 nm using a microtiter plate reader (Spectramax M5, Molecular Devices). Alternatively, an overnight culture of E. coli was diluted 1:1000 fold and incubated with varying amounts of berberine sulfate for 20 h before growth was determined. All experiments were performed at least twice. Viability of berberine sulfate-treated (24 h) cells was evaluated by spotting 3 mL serial dilutions (10 22 -10 27 ) of bacteria on a petri dish and incubation overnight at 30 uC.
Bacterial morphology was visualized using a Zeiss AxioVert 200 microscope and images were obtained using a 206 objective.

General chemistry methods for library synthesis
Reactions were carried out under a nitrogen atmosphere with dry, freshly distilled solvents under anhydrous conditions, unless otherwise noted. Yields refer to chromatographically and spectroscopically homogenous materials, unless otherwise stated. Reactions were monitored by thin-layer chromatography (TLC) carried out on 0.25-mm EMD silica gel plates (60F-254) using UV-light (254 nm). Flash chromatography separations were performed on Silicycle silica gel (40-63 mesh). Purity analyses were performed using HPLC (254 nm).

General synthetic procedure for library compounds
A stirring solution of aldehyde (1.0 equiv) in MeOH at 25uC was treated with carboxylic acid (2.0 equiv), amine (2.0 equiv) and isonitrile (2.0 equiv). The solution was heated to reflux, and stirred for 24 h. The solution was then cooled to 25uC and concentrated in vacuo. The crude residue was purified via flash column chromatography (10-50% EtOAc in hexanes) to afford the purified product. For characterization data, see references [39][40].

Genomic organization of the major cell division genes in wBm
The bacterial cell-division pathway has been extensively studied in E. coli and several essential proteins have been identified [17,19]. Many of the genes encoding putative orthologs of these proteins are also present in wBm (Table 1). A total of 18 major cell division genes were identified in wBm genome (Table 1), including ftsZ, ftsA, ftsI, ftsK, ftsQ and ftsW, which are known to be essential for cell division [17]. These wBm genes were mapped and found to be more scattered throughout the genome, in comparison with their E. coli homologs. In E. coli the majority of genes were found in one major operon, with the remaining 5 genes distributed randomly. Of these, FtsZ was one of the most highly conserved essential proteins possessing 43% identity to Ec-FtsZ (Table 1). Wolbachia ftsA, ftsI, ftsK, ftsQ and ftsW were less related (13-34%) to the E. coli homologs. Some previously described essential cell division genes in E. coli (including ftsB, ftsL, ftsN and ZipA) were not found in wBm, indicating that there are differences in the cell division machinery present in free living E. coli and intracellular Wolbachia.
Sequence analysis of wBm-ftsZ wBm-ftsZ exists as a single gene on the chromosome and is 1182 bp in length. It encodes a 394-amino acid protein with a predicted molecular mass of 42 kDa containing four distinct domains characteristic of FtsZ proteins. These comprise the variable N-terminal domain, a highly conserved core region, variable spacer, and a C-terminal conserved domain. The core region contains the highly conserved catalytic aspartate residue [41][42] and the GGGTGTGA motif (8 residues see [41,43]), which are responsible for GTP hydrolysis and required for polymerization of the protein.
The C-terminal region is not required for assembly, but is essential for interactions with the cell division proteins FtsA, FtsW and ZipA [17]. A similar organization was also found in the insect Wolbachia, wMel-FtsZ (NP_966481) and wAlB-FtsZ (JN616286). The FtsZ proteins of Wolbachia from different hosts share 89-91% identity and 43% identity to E. coli FtsZ proteins, with a substantially lower level at the carboxyl-terminal region (17.2% identity).

Analysis of wBm-ftsZ expression during the life cycle of B. malayi
Wolbachia have been identified in all developmental stages of B. malayi, from studies on individual worms and isolates from regions endemic for lymphatic filariasis [2][3][4]. To determine the relative expression of wBm-FtsZ throughout the parasite life cycle and validate its suitability as a drug target, wBm-ftsZ mRNA expression was analyzed by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Relative levels of ftsZ expression (ratio of Wolbachia ftsZ to 16S rRNA) and abundance of Wolbachia in B. malayi (ratio of Wolbachia 16S to B. malayi 18S rRNA) were calculated for each RNA sample.
wBm-ftsZ was found to be expressed throughout all stages examined (adult female and male worms, microfilariae, third-and fourth-stage larvae). Moreover, wBm-ftsZ/16S ratios were found to be increased substantially following infection of the mammalian host since levels were significantly higher (p value,0.001) in fourth-stage larvae and adult female worms compared to the vector-derived infective third-stage larvae. The wBm-ftsZ/16S ratio was also higher in microfilariae compared with the vectorderived third-stage larvae, but was significantly lower than the ratios obtained for fourth-stage and adult female worms. Of the various developmental stages examined, the lowest level of wBm-ftsZ expression was found in male worms ( Figure 1A). No DNA contamination was detected in controls consisting of samples processed in the absence of reverse transcriptase. Wolbachia 16S rRNA/B. malayi 18S rRNA ratios were also determined to measure the relative abundance of bacteria in different stages of B. malayi ( Figure 1B). Wolbachia was found to be most abundant in fourth stage larvae and adult female worms and least abundant in infective third stage larvae, indicating a massive multiplication of Wolbachia soon after infection of the mammalian host. Taken together, these data indicate that while wBm-ftsZ is expressed in all stages, gene activity and bacterial multiplication is most pronounced in fourth-stage larvae and adult females.

Expression and purification of recombinant wBm-FtsZ
Recombinant wBm-FtsZ was expressed in E. coli with a His-tag at the C-terminus and purified by nickel-affinity chromatography (Figure 2A). Optimum conditions for production of soluble recombinant wBm-FtsZ involved growth of cultures at 37uC until the OD 600 reached 0.6, followed by induction with 0.1 mM IPTG overnight at 16uC. Purified protein was eluted with 100 mM imidazole. The apparent molecular weight of 43 kDa (Figure 2A) was consistent with the predicted molecular size of wBm-FtsZ with an N-terminal His-tag. For comparative studies, E. coli FtsZ (41 kDa) was also expressed and purified in a similar manner ( Figure 2B).

Recombinant wBm-FtsZ has GTPase activity
GTPase activity was measured using an enzyme-coupled assay involving pyruvate kinase and lactate dehydrogenase [36]. GTP hydrolysis was determined by measuring the decrease in fluorescence emission following oxidation of nicotinamide adenine dinucleotide (NADH) to NAD ( Figure 3A). As Figure 3B shows, recombinant wBm-FtsZ was found to possess GTPase activity. Moreover, the specific activities for wBm-FtsZ and Ec-FtsZ were comparable (0.1860.012 mmolmmin 21 mg 21 and 0.2260.015 mmol min 21 mg 21 , respectively). Inhibition of wBm-FtsZ GTPase activity using the plant alkaloid berberine Berberine, an alkaloid natural product, is a known inhibitor of the GTPase activity of FtsZ in E. coli [33,44]. Thus, we were interested in examining the generality of berberine's GTPase inhibitory activity against wBm-FtsZ. As Figure 4 shows, dosedependent inhibition (25-1000 mM) was found with an IC 50 value of 320 mM. E. coli FtsZ [33,44] was included for comparison, and an IC 50 value of 240 mM was observed (Figure 4). Since wBm-FtsZ possesses all but one of the key residues proposed in the binding of E. coli FtsZ to berberine (lysine instead of glycine at position 183 of Ec-FtsZ), this may account for the higher concentration of berberine required to inhibit 50% of wBm-Ftsz's GTPase activity.
The effect of berberine on the motility and microfilariae production of B. malayi in vitro Since filarial Wolbachia remain unculturable, we were unable to evaluate the direct effect of berberine on the endosymbiont. Therefore, we examined the indirect effect of the drug on adult female worm. As Figure 5A shows, berberine (10-40 mM) had adverse effects on the motility of adult female B. malayi worms, as well as microfilariae production ( Figure 5B) when compared to untreated controls. Two days after treatment with berberine (40 mM), female worms showed almost no movement and the production of microfilaria had virtually ceased. Berberine at 20 mM was comparable to 10 mM of doxycycline in terms of effect on female worm motility. Reduction in adult female motility coincided with a decrease in microfilariae production. Similarly, motility of the freshly released microfilaria was decreased when berberine was present, with some effect observed at the lowest concentration (5 mM) tested ( Figure 5C). On the other hand, male worms were more resistant to the effects of the drug with limited reduction in motility observed following treatment with berberine (5-40 mM) for 6 days ( Figure 5D). However, treatment with 100 mM berberine for 24 h did completely paralyze male worms (data not shown). Doxycycline (10 mM) had a comparable affect on the motility of male and female worms.

The effect of berberine on E. coli growth, morphology and viability
To demonstrate that berberine's in vitro GTPase inhibitory activity and anti-parasitic activity correlates with its known antibacterial activity, studies were performed on E. coli strain ER1613. Berberine is known to act as a substrate for the multidrug resistance efflux pumps and ER1613 contains a mutation in the acrA gene, which inactivates the multidrug efflux pump [45]. Overnight incubation of ER1613 with 0-100 mM berberine showed a dose-dependent effect with complete inhibition of bacterial growth observed at 60 mM ( Figure 6A). Similarly, no growth was evident when experiments were initiated with greater bacterial densities and the cells were treated with 50 mM berberine for up to 5 h ( Figure 6B). Treatment with berberine resulted in the filamentous phenotype ( Figure 5C) typically observed in ftsZ mutant strains [46], indicating that berberine was inhibiting cell division. Moreover, the presence of elongated bacteria also correlated with decreased growth and viability. Viability was also evaluated by ability to form colonies on an agar plate. Berberine sulfate-treated (24 hours) cells produced substantially fewer colonies ( Figure 6D), compared to untreated controls. Untreated bacteria had approximately 4610 5 -fold growth in 24 h, whereas bacteria treated with 40 mM berberine had 4610 2 -fold growth. At concentrations of 80 mM and higher, the treated bacteria failed to produce viable colonies ( Figure 6D), demonstrating that without active replication E. coli die.

Identification of new inhibitors of wBm-FtsZ GTPase activity
To initiate a campaign to identify molecularly unique inhibitors of wBm-FtsZ GTPase activity, a library of small molecules based on naphthalene, quinoline and biphenyl core scaffolds were examined [39][40] (Figure 7A). The library was constructed using Ugi multicomponent reaction chemistry, and each compound consists of a flat aromatic scaffold for enhanced p-stacking interactions decorated with varying diversity elements (R 1 -R 4 in Figure 7A). Importantly, these scaffold motifs are also found in berberine ( Figure 7B) and known FtsZ inhibitors [17,[29][30][31][32][33]. The ,500-member library was screened using the wBm-FtsZ GTPase assay, and 13 compounds with greater than 30% inhibition at 100 mM were identified. From these screening efforts, compounds AV-C6 and N938 ( Figure 7C) emerged as leading hits, and each showed dose-dependent inhibition of wBm-FtsZ ( Figure 8A). AV-C6 and N938 were also examined for inhibition of the E. coli FtsZ enzyme ( Figure 8A). As shown in Figure 8A, both compounds inhibited Ec-FtsZ activity although each was slightly less potent compared to the inhibitory activity against wBm-FtsZ.
Structure-activity relationship (SAR) studies were then performed on N938 as this compound showed the most potential in dose response experiments. In addition to identifying compounds with enhanced potency, we were also interested in exploring the possibility of tuning down any inhibitory activity against Ec-FtsZ in order to obtain a more specific Wolbachia FtsZ inhibitor. A series of analogues were synthesized with varying aromatic side chains (R 3 in Figure 7A). As shown in Figure 8B, both goals were met: N982 with an ortho-chloro substituent ( Figure 7D) showed enhanced potency in the wBm-FtsZ assay and N983 with a para-cyano substituent ( Figure 7D) showed some specificity for wBm-FtsZ over that from E. coli. Future SAR studies should enable the discovery of compounds with both enhanced inhibitory properties and specificity. Finally, as the solubility of these compounds is poor, 100% inhibition of FtsZ with this scaffold was not possible and true IC 50 values could not be obtained. Scaffold modification and/ or hopping strategies will be investigated in the future to afford enhanced solubility.

Discussion
The use of antibiotics targeting the Wolbachia endosymbionts of filarial parasites has been validated as an approach for controlling filarial infection in animals and humans. As a result, there is considerable interest in identifying new compounds that specifically target the obligate bacterial endosymbiont. In the present study, we investigated the cell division pathway in wBm to identify new drug targets that may be exploited for the development of new antifilarial therapies. Filamenting temperature sensitive (fts) genes produce many of the proteins essential for cell division in E. coli [17]. In wBm, we identified the majority of core genes that are indispensable to cytokinesis including ftsA, ftsI, ftsK, ftsQ, ftsW and ftsZ.
Interestingly, ftsB, ftsL, ftsN and ZipA were not found in wBm. ZipA is a bitopic membrane protein with a large cytoplasmic domain that binds and bundles FtsZ protofilaments in vitro and helps to stabilize the Z ring in vivo. FtsN is a core component of the divisome that accumulates at the septal ring at the initiation of the constriction process. The C-terminal SPOR domain specifically recognizes a transient form of septal murein, which helps trigger and sustain the constriction process. However, in E. coli, it has been found that alterations in FtsA can compensate for the absence of ZipA, FtsK [47] and FtsN [48] and a gain-of-function FtsA variant, FtsA*(R286W), efficiently stimulates cell division in the complete absence of ZipA [47]. Thus, Wolbachia FtsA may function like the mutant FtsA, as an alanine residue is present in the same position.
ftsB, ftsL, ftsN and ZipA are also absent in some important bacterial pathogens including certain Gram-negative (Neisseria spp., Bordetella pertussis, Helicobacter pylori, Chlamydia spp.) and Grampositive (Mycobacterium tuberculosis) bacteria and cell wall-lacking (Mycoplasma pneumoniae) organisms [17]. It is likely that this reflects the reduced genome size present in these intracellular bacteria.
FtsZ is the most highly conserved essential bacterial cell division protein and is present in all bacteria except Chlamydia spp [17]. We determined that wBm-FtsZ shares substantial similarity (43% identity) to the highly characterized E. coli FtsZ protein and is highly similar (,90% identity) to insect Wolbachia FtsZ proteins. While the majority of wBm genes are expressed in a stage-specific manner [49], wBm-ftsZ was found to be expressed in both male and female worms as well as in all larval stages examined. It was not surprising to find wBm-ftsZ expressed throughout the entire lifecycle of the parasite since the bacterial Z-ring is known to exist in a state of dynamic equilibrium in order to fulfill its many roles in the cell. Using fluorescence recovery after photo bleaching (FRAP), the E. coli Z-ring was found to continually remodel itself with a halftime of 30 seconds with only 30% of cellular FtsZ Figure 3. GTPase activity of recombinant wBm-FtsZ. Panel A, activity was determined indirectly by measuring a decrease in NADH concentration by its absorbance at 340 nm. FtsZ hydrolyzes GTP into GDP and inorganic phosphate. The GDP product is used as a substrate by pyruvate kinase (PK) in the presence of phosphoenol pyruvate (PEP) to yield GTP and pyruvic acid as products. Pyruvic acid is used as a substrate in the presence of NADH by lactate dehydrogenase (LDH) to generate lactate and NAD. The consumption of NADH is proportional to GTPase activity. Panel B, comparison of the GTPase activity of wBm-FtsZ and Ec-FtsZ. A control without enzyme was also included. Activity was indicated by a decrease of NADH measured by absorbance at 340 nm. doi:10.1371/journal.pntd.0001411.g003 present in the ring with continuous and rapid exchange of subunits within a cytoplasmic pool [17]. E. coli ftsZ transcription analysis has revealed that the rate of ftsZ expression is constant with a sudden doubling at a specific cell age, suggesting that ftsZ expression is regulated [50]. Similarly, we observed up-regulation of wBm-ftsZ gene expression in fourth-stage larvae and adult female worms with microfilariae likely contributing to the increased expression in the latter case. While the lowest levels of gene expression were evident in adult males, FtsZ protein was easily detected in proteomic analyses of male worms [49]. In general, the gene expression pattern of ftsZ correlated with bacterial multiplication. The increased bacterial multiplication in the worm during early infection of the mammalian host and embryogenesis is in agreement with an earlier study [4]. These data are consistent with the third-and fourth-stage larval stages, and embryogenesis being particularly sensitive to the effects of Figure 5. Effect of berberine sulfate on B. malayi parasites in culture. Motility of adult female (A) and male (D) worms, and microfilariae (C) was examined following 6 days exposure to varying amounts (5-40 mM) of berberine sulfate. 10 mM doxycycline was included as a control. Motility was scored as described [38] and expressed as % of motility relative to motility scored on day 0 of the experiment. Micofilariae production (B) was determined at each time point by counting the number of microfilaria present in 1 mL spent culture media. The data obtained from triplicate samples are expressed as a mean 6 standard deviation. doi:10.1371/journal.pntd.0001411.g005 Inhibition of Wolbachia FtsZ for Drug Discovery www.plosntds.org antibiotic treatment [4,51]. This result indicates that ftsZ gene expression could be used as a marker to monitor Wolbachia multiplication in the filarial parasite much like the ftsZ gene in the intracellular bacterium Candidatus Glomeribacter gigasporarum that resides in the mycorrhizal fungus Gigaspora margarita [43].
Molecular studies have established the importance of conserved amino acids in the FtsZ protein that when changed results in ftsZ mutants blocked at different stages of cell division [42,46,[52][53][54][55]. wBm-FtsZ possesses the key residues and conserved GTP-binding pocket required for GTPase activity. Our functional analysis revealed that the GTPase activities of recombinant wBm-FtsZ and Ec-FtsZ are similar, and both proteins are sensitive to the plant alkaloid berberine. Most of the residues in Ec-FtsZ that are thought to bind berberine and inhibit FtsZ GTPase activity are also present in wBm-FtsZ. An earlier detailed study in E. coli determined that the target of this commonly used compound is FtsZ [33]. Plants containing berberine have been used in traditional Chinese and Native American medicine to treat many infectious diseases and the sulfate, hydrochloride and chloride forms are used in Western pharmaceutical medicine as antibacterial agents [56]. It is active against a number of Gram-positive and Gram-negative pathogenic bacteria, including drug resistant Mycobacterium tuberculosis [57] and Staphylococcus aureus [58]. Our experiments in E. coli demonstrate that berberine has both bacteriostatic and bacteriocidal effects. Since filarial Wolbachia remain unculturable, we were unable to evaluate the direct effect of berberine on the endosymbiont. However, following berberine treatment, we did observe reductions in adult female worm and microfilariae motility and microfilariae production. On the other hand, we did not see any effect on male worms, which had the lowest level of wBm-ftsZ gene expression. We examined berberineand doxycycline-treated worms for Wolbachia load by qPCR analysis and did not observe a significant difference between control and treated parasites. A similar result was also found in a study evaluating the effects of globomycin and doxycycline on filarial Wolbachia, and the authors [59] suggested several possibilities which can also apply to our study, namely: the Wolbachia qPCR assay may not have sufficient sensitivity to detect effects on Wolbachia load over this time frame in nematodes, inhibition of FtsZ is sufficient to affect nematode motility and viability independent of or prior to any effect on Wolbachia load, and/or a direct effect of berberine on nematode motility and viability and alternative mechanisms of action. Nonetheless, our results suggest that FtsZ inhibitors that operate via inhibition of enzyme activity including natural products [28,[30][31][32][33]53] and synthetic molecules [29,60] may have also activity against wBm-FtsZ.
To complement the berberine studies, a library of naphthalene-, quinoline-and biphenyl-based compounds constructed using Ugi multicomponent reaction chemistry was examined for the discovery of new and ultimately highly specific antagonists of either E. coli or Wolbachia FtsZ. Of interest, compounds based on similar scaffolds have already been demonstrated as potent FtsZ inhibitors [17,[29][30][31][32][33]. From our screening efforts, the (6-{butylcarbamoyl-[(aryl)-(butylcarbonyl)-amino]-methyl})-naphthen-2-ol scaffold ( Figure 7A, C) emerged as an antagonist of both E. coli and Wolbachia FtsZ. Interestingly, from basic SAR studies it appears that modification of the aryl substituent on the scaffold may afford selectivity for Wolbachia FtsZ, a key element of our initial goal. Additional compounds are currently being prepared to examine this possibility. Although not discussed here, compounds based on our lead scaffold had no effect on growth or viability in E. coli. Based on these findings and their potency in the in vitro assays, it is plausible that penetrability or metabolism issues are to blame for their attenuated activity. Finally, the solubility of these compounds is also poor precluding measurement of true IC 50 values. Further iterations of chemical synthesis will be necessary to address these potential liabilities.
While we have focused on assaying the GTPase activity of wBm-FtsZ using a medium-to high-throughput coupled enzyme assay for the discovery of inhibitors that target cell division in Wolbachia, it is also possible to screen for compounds that would target wBm-FtsZ via other mechanisms of action. FtsZ is considered a distant functional relative of the mammalian cytoskeletal protein btubulin [61][62][63]. Microtubule formation is a major target in cancer chemotherapy and the anticancer drug Taxol binds to b-tubulin and blocks cell division by interfering with microtubule formation. Interestingly, the FtsZ inhibitor PC190723 [60] operates by a similar mechanism and more recently, novel inhibitors of B. subtilis cell division have been identified in an in vitro FtsZ protofilaments polymerization assay [64]. Importantly, significant differences exist in the active sites in tubulin and FtsZ polymers, and several small molecule inhibitors of FtsZ have been identified [65] that do not inhibit tubulin [66][67]. Tubulin is also the target of the broadly anti-parasitic benzimidazole drugs [68][69], which have been used extensively to control soil-transmitted nematodes [70][71].
FtsZ is also responsible for recruiting and coordinating more than a dozen other cell division proteins at the midcell site of the closing septum [18][19]21,72]. Many of these interactions are essential and it has been suggested that they might also be useful targets, particularly in light of developments in the discovery of small molecule inhibitors of protein-protein interactions [17,73-  74]. Therefore, it might be feasible to screen for inhibitors of the interactions between wBm-FtsZ and its various binding partners that modulate its polymerization. Another Wolbachia cell division protein worth considering for drug discovery is FtsA, as this protein also possesses enzymatic activity and contains an ATPbinding site that might be targeted with drug-like molecules. Moreover, this protein is essential in E. coli [75] and Streptococcus pneumoniae [76].
In summary, we have investigated the cell division pathway in wBm and determined that it possesses a FtsZ protein with GTPase activity. We demonstrated that the activity is inhibited by berberine and identified small molecule inhibitors in a highthroughput screen. Furthermore, berberine was found to have adverse affects on B. malayi adult worm and microfilariae motility, and reproduction. Our results support the discovery of selective inhibitors of Wolbachia FtsZ as a new therapeutic approach for filariasis.