Fig 1.
Proteome analysis of differentially expressed proteins in tachyzoite and tachyzoite to bradyzoite transformation stages of T. gondii infection.
(A) Heat map shows the differentially expressed proteins in the three groups. (B) Volcano plot shows differentially expressed proteins between the C8-TA group and the C8 group. (C) Volcano plot shows differentially expressed proteins between the C8-BR group and the C8-TA group.
Fig 2.
Location and functional classification of differentially expressed proteins between the C8-TA group and the C8 group.
(A) Location of subcellular structures of differentially expressed proteins between the C8-TA group and the C8 group. (B) KOG classification of differentially expressed proteins between the C8-TA group and the C8 group. (C) GO enrichment analysis of differentially expressed proteins between C8-TA vs C8 group included biological process, cellular composition, and biological function.
Fig 3.
GO and KEGG pathway enrichment analysis of up-regulated and down-regulated proteins between C8-TA group and C8 group.
(A) GO enrichment analysis of up-regulated proteins. (B) GO enrichment analysis of down-regulated proteins. (C) KEGG pathway enrichment analysis of up-regulated proteins. (D) KEGG pathway enrichment analysis of down-regulated proteins.
Fig 4.
Subcellular localization and functional classification of differentially expressed proteins between C8-BR group and C8-TA group.
(A) The location of subcellular structures of all differentially expressed proteins. (B) The location of subcellular structures of up-regulated proteins. (C) The location of subcellular structures of down-regulated proteins. (D) The KOG classification of up-regulated proteins. (E) The KOG classification of down-regulated proteins.
Fig 5.
GO and KEGG pathway enrichment analysis of up-regulated and down-regulated differentially expressed proteins between C8-BR group and C8-TA group.
(A) GO enrichment analysis of up-regulated proteins. (B) GO enrichment analysis of down-regulated proteins. (C) KEGG pathway enrichment analysis of up-regulated proteins. (D) KEGG pathway enrichment analysis of down-regulated proteins.
Fig 6.
Comparative analyses of label-free proteomics and PRM results for 18 screened differentially expressed proteins.
Fig 7.
Verification of the differentially expressed proteins in different stages (tachyzoite and bradyzoite) of T. gondii infection.
(A) Western blotting analysis was used to detect the protein expression level of differentially expressed proteins in C8 group and C8-TA group. (B) Histogram analysis of the western blotting results in C8 group and C8-TA group. Statistical significance was analyzed using an unpaired Student’s t-test (***p < 0.001). (C) Western blotting analysis was used to detect the protein expression level of differentially expressed proteins in C8-TA group and C8-BR group. (D) Histogram analysis of the western blotting results in C8-TA group and C8-BR group. Statistical significance was analyzed using an unpaired Student’s t-test (***p < 0.001).