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Fig 1.

Overall study design of exposure experiments.

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Fig 2.

High-Throughput Screening System (HITSS).

The HITSS chambers are modular, consisting of metal cylinders attached to clear cylinders. Treated netting is placed inside the metal cylinder and mosquitoes introduced into the clear chamber to prevent making direct contact with the chemical but allowing for chemical exposure (by permission of the American Mosquito Control Association) [25].

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Table 1.

Sample size of Aedes aegypti1 (exposed and unexposed)2 observed during study.

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Fig 3.

A. Bioassay test arena. Each bioassay test arena is composed of 30x30x30cm Plexiglas containing four experimental oviposition sites. B. Experimental oviposition site. Black plastic cup containing either attractant bacterial beads or water only (matched control) covered by a wire mesh ‘sticky-screen’ that captured pigment powder marked gravid Ae. aegypti.

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Table 2.

Effects of transfluthrin (n = 780) or solvent (n = 780) exposure on gravid Ae. aegypti1 attraction to experimental oviposition sites.

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Table 2 Expand