Table 1.
Isolation source, origin, of strains analyzed.
Table 2.
Oligonucleotide padlock probes and probe-specific primers used for species identification with RCA.
Figure 1.
Specificity of rolling circle amplification probes.
Agarose gel electrophoresis analysis of rolling circle amplification products. Positives probe signal seen as band pattern was only present with matched template–probe mixtures. Probe names are indicated on the top of the gel. Lanes; 1 M. mycetomatis CBS 109801T, 2 M. tropicana CBS 201.38T, 3 M. pseudomycetomatis CBS 129177T, 4 M. fahalii CBS 129176T, 5 T. grisea CBS 332.50T, 6 F. senegalensis CBS 196.79T, 7 F. tompkinsii CBS 200.70, 8 M. romeroi CBS 252.60T, M DNA ladder.
Figure 2.
Madurella mycetomatis identification by RCA.
Gel representation of rolling circle amplification reaction using Madurella mycetomatis probe (MYC) for strains recovered from mycetoma patient of origin: lane 1–18 Sudan; lane 19, 20 Mali; lane 21, 22 India; lane 23 negative control water; lane M ladder.
Table 3.
Rolling circle amplification results of analysed strain.
Figure 3.
Identification time of species using rolling circle amplification (RCA) and sequencing of ITS.