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The antiparasitic drug niclosamide inhibits dengue virus infection by interfering with endosomal acidification independent of mTOR

Fig 6

Niclosamide treatment reduces viral replication and increases the survival rate in suckling mice during DENV infection.

(A) With or without niclosamide (Niclo; 2 mg/kg or 5 mg/kg) co-treatment, seven-day-old ICR suckling mice were inoculated with DENV2 by concurrent intracranial (2.5 × 105 pfu) and intraperitoneal (7.5 × 105 pfu) injections. (B) Western blot analysis of viral NS3 and NS1 protein expression. (C) Plaque assay of viral replication in the brains at 9 days post-infection. β-actin served as the internal control. The relative ratios of the measured proteins compared to β-actin are also shown. The quantitative data are depicted as the mean ± SD of three independent experiments. ** P < 0.01 and *** P < 0.001. Additionally, time-kinetic changes in (D) body weights, (E) clinical scores, and (F) survival rates were measured. P value is shown.

Fig 6

doi: https://doi.org/10.1371/journal.pntd.0006715.g006