Development and evaluation of a novel high-throughput image-based fluorescent neutralization test for detection of Zika virus infection
A total of 226 serum samples, among then negative and positive for flavivirus and non-flavivirus infections were examined. The neutralization titers that inhibit 90% of viral infection (NT90) are shown in the graph. Other infections: Leptospirosis, CMV, EBV, Hantavirus, Toxoplasmosis, VDRL. Dashed lines represent the test cut off: negative when NT90 <10, inconclusive when NT90 ≥10 and <20, and positive when NT90 ≥20. Symbols in blue indicate paired samples.