Zika virus inhibits eIF2α-dependent stress granule assembly
A. Vero cells were infected with ZIKV with an MOI of 0.5 or mock-infected and treated at 24 hpi with 50 nM PatA for 2 h to induce cellular stress. B. U2OS GFP-G3BP1-expressing cells were infected with ZIKV or mock-infected and treated at 24 hpi with 1 mM Se for 2 hours. SG assembly was determined by IF/LSCM staining for the SG markers TIAR and eIF4G (Vero cells) or G3BP-1 and TIAR (U2OS cells) and infected cells were identified by the presence of the viral protein NS1. Blue arrows: uninfected cells; red arrows: infected cells. C. At least 150 cells in each condition were analyzed. Cells with at least 3 SG were considered positive. Data are presented as mean ± SD from 3 independent experiments.