A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus
A modified virus capture ELISA was conducted to assess the ability of plasma from 46 individuals to block the binding of P1F12 to whole ZIKV. Captured ZIKV was incubated with 1/10 diluted plasma from naïve (US and Brazil) and DENV+, YFV+ or ZIKV+ volunteers prior to addition of purified P1F12. Viral infection was determined by RT-PCR. ZIKV-bound P1F12 was detected using an HRP-conjugated secondary Ab specific for the rhesus IgG1 Fc region of recombinant P1F12. ZIKV+ (blue circles), but not ZIKV- (gray circles) plasma inhibited binding of P1F12 mAb to ZIKV.