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A human inferred germline antibody binds to an immunodominant epitope and neutralizes Zika virus

Fig 3

Plasmablast-derived ZIKV-specific mAbs had low SHM levels.

A) Number of amino acid mutations from heavy and light chain germline sequences. B) Amino acid alignment of P1F12 to germline genes shows no mutations downstream of cloning primer. Dots “.” indicate sequence identity to the germline gene (shown in each row). Dashes “-” indicate that the Ab does not align to the annotated germline gene sequence on that position. The CDR-H3 sequence is indicated in blue. C) Nucleotide alignment of P1F12 CDR-H3 junction to germline genes. Boxes indicate junctional diversity between V and D (N1), and D and J (N2) gene segments. Framework (FWR) and complementarity-determining regions (CDRs) boundaries are directly annotated on top of the Ab sequence. Antibody regions were determined using IMGT/V-QUEST.

Fig 3

doi: https://doi.org/10.1371/journal.pntd.0005655.g003