The phosphatidylinositol-3-phosphate 5-kinase inhibitor apilimod blocks filoviral entry and infection
BSC-1 cells were grown overnight on 35 mm glass bottom dishes (MatTek). The medium on the cells was replaced with serum-free medium containing 0.05 μM TopFluor Cholesterol (Avanti) plus (A) DMSO (mock), (B) 5 μM U18666A, or (C) 0.2 μM apilimod. The cells were incubated for 18 h at 37°C, washed once with PBS, and imaged in cell imaging medium. Images are representative of all observed fields (25 per treatment).