The phosphatidylinositol-3-phosphate 5-kinase inhibitor apilimod blocks filoviral entry and infection
BSC-1 cells grown overnight in 35 mm glass bottom dishes (MatTek) were treated for 3 h at 37°C with (A) DMSO (mock), (B) 25 nM bafilomycin (Baf), or (C) 0.2 μM apilimod. Acridine Orange (6.6 μg/mL) was added, and the cells were further incubated at 37°C for 20 min. The cells were then washed and imaged. Scale bars, 10 μm. Images are representative of all observed fields (30 for Baf and 35 for mock- and apilimod-treated cells, respectively).