Characterization of a novel microfilarial antigen for diagnosis of Wuchereria bancrofti infections
Fig 3
Immunolocalization of BmR1 homologues.
This figure shows immunohistochemical results from worm sections that were processed as described in the Methods. A B. malayi female worm section stained with normal mouse sera showed no labeling (A). B. malayi female worm sections stained with anti-Wb-Bhp-1 demonstrated no staining in morula (B) or pretzel stage larvae (C) but staining of stretched Mf (DE). B. malayi L3 stage demonstrated minimal staining with normal mouse sera (F) and minimal staining in a diffuse pattern with anti-Wb-Bhp-1 sera (G). O. volvulus nodule stained with anti-Wb-Bhp-1 demonstrated staining of Mf within the nodule (H). Annotations: Mf (arrow), uterus (ut), and intestine (i), which has intrinsic alkaline phosphatase. Scale bar indicates 20 micrometers.