Establishment of Fruit Bat Cells (Rousettus aegyptiacus) as a Model System for the Investigation of Filoviral Infection

Verena Krähling; Olga Dolnik; Larissa Kolesnikova; Jonas Schmidt-Chanasit; Ingo Jordan; Volker Sandig; Stephan Günther; Stephan Becker

doi:10.1371/journal.pntd.0000802

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Peer-reviewed

Research Article

Establishment of Fruit Bat Cells (Rousettus aegyptiacus) as a Model System for the Investigation of Filoviral Infection

Verena Krähling,

Affiliation Institut für Virologie, Philipps-Universität Marburg, Marburg, Germany
⨯
Olga Dolnik,

Affiliation Institut für Virologie, Philipps-Universität Marburg, Marburg, Germany
⨯
Larissa Kolesnikova,

Affiliation Institut für Virologie, Philipps-Universität Marburg, Marburg, Germany
⨯
Jonas Schmidt-Chanasit,

Affiliation Institut für Virologie, Bernhard-Nocht-Institut für Tropenmedizin, Hamburg, Germany
⨯
Ingo Jordan,

Affiliation ProBioGen AG, Berlin, Germany
⨯
Volker Sandig,

Affiliation ProBioGen AG, Berlin, Germany
⨯
Stephan Günther,

Affiliation Institut für Virologie, Bernhard-Nocht-Institut für Tropenmedizin, Hamburg, Germany
⨯
Stephan Becker

* E-mail: becker@staff.uni-marburg.de

Affiliation Institut für Virologie, Philipps-Universität Marburg, Marburg, Germany
⨯

Establishment of Fruit Bat Cells (Rousettus aegyptiacus) as a Model System for the Investigation of Filoviral Infection

Verena Krähling,
Olga Dolnik,
Larissa Kolesnikova,
Jonas Schmidt-Chanasit,
Ingo Jordan,
Volker Sandig,
Stephan Günther,
Stephan Becker

Published: August 24, 2010
https://doi.org/10.1371/journal.pntd.0000802

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Problems with fruit bat cell line used in this manuscript

Posted by stepbecker on 06 May 2011 at 18:49 GMT

We have learnt from another group using the same fruit bat cell line as used in this article (RO6E) that the cells were probably contaminated by another cell line (Vero, a monkey kidney cell widely used to propagate viruses). Our analyses confirmed a contamination with Vero cells occured before we started our study to characterize filovirus infection in RO6E cells. Investigation of early passages of the cell line indicated that pure passages are available and those are now used to repeat the experiments shown in the paper. First results indicate that the early passages are susceptible to productive Ebola virus infections.

Further results we will posted as soon as available.

Stephan Becker

Philipps-Universität Marburg
Institut für Virologie

No competing interests declared.

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Filoviruses
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Fruit bats
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Transfection
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Establishment of Fruit Bat Cells (Rousettus aegyptiacus) as a Model System for the Investigation of Filoviral Infection

Establishment of Fruit Bat Cells (Rousettus aegyptiacus) as a Model System for the Investigation of Filoviral Infection

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Problems with fruit bat cell line used in this manuscript

Posted by stepbecker on 06 May 2011 at 18:49 GMT